PMID- 37539185
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230805
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 7
DP  - 2023 Jul
TI  - Circular RNA circSLC7A11 contributes to progression and stemness of laryngeal 
      squamous cell carcinoma via sponging miR-877-5p from LASP1.
PG  - e18290
LID - 10.1016/j.heliyon.2023.e18290 [doi]
LID - e18290
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) belongs to tumors of head 
      and neck. Circular RNA circSLC7A11 functions as oncogenes in various tumors. 
      However, the role of circSLC7A11 in LSCC remains largely unknown. Here, we aimed 
      to clarify the circSLC7A11 function in LSCC. METHODS: Relevance between 
      circSLC7A11 expressions and LSCC clinicopathological was checked using 
      chi-square. Relevance between circSLC7A11 expressions and LSCC patients' survival 
      time was validated using Kaplan-Meier analysis. CircSLC7A11 expressions in LSCC 
      tissues and cells were determined using quantitative real-time PCR. CircSLC7A11 
      functions in LSCC were examined by Cell Counting Kit-8, EdU analysis, Western 
      blot, flow cytometry, sphere formation assay, and Transwell analysis. Meanwhile, 
      circSLC7A11 mechanism in LSCC was determined using dual-luciferase reporter 
      analysis, RNA pull-down, RNA Immunoprecipitation. RESULTS: CircSLC7A11 was highly 
      expressed in LSCC, and high circSLC7A11 expressions were interrelated to the TNM 
      stage. Also, LSCC patients with high circSLC7A11 owned shorter overall survival. 
      Functional studies revealed that circSLC7A11 knockdown reduced LSCC cell 
      proliferation, migration, invasion, stemness characteristics, and enhanced cell 
      apoptosis. Mechanistic study data corroborated that circSLC7A11 targeted 
      miR-877-5p, miR-877-5p targeted LASP1. LASP1 was negatively interrelated to 
      miR-877-5p and was positively interrelated to circSLC7A11 in LSCC tissues. Also, 
      circSLC7A11 knockdown reduced the LASP1 levels, and miR-877-5p inhibitor 
      co-transfection reversed this reduction. Rescue assays further demonstrated that 
      circSLC7A11 accelerated LSCC through miR-877-5p/LASP1. CONCLUSION: CircSLC7A11 
      exerted oncogenic functions in LSCC by miR-877-5p/LASP1, hinting that circSLC7A11 
      was a novel biomarker for LSCC.
CI  - (c) 2023 The Authors.
FAU - Yu, Wenjie
AU  - Yu W
AD  - Department of Otolaryngology Head and Neck Surgery, First Hospital of Shanxi 
      Medical University, China.
FAU - He, Xiaoling
AU  - He X
AD  - Department of Otolaryngology Head and Neck Surgery, First Hospital of Shanxi 
      Medical University, China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Department of Otolaryngology Head and Neck Surgery, First Hospital of Shanxi 
      Medical University, China.
FAU - Huang, Fuhui
AU  - Huang F
AD  - Department of Otolaryngology Head and Neck Surgery, First Hospital of Shanxi 
      Medical University, China.
LA  - eng
PT  - Journal Article
DEP - 20230714
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10393633
OTO - NOTNLM
OT  - CircSLC7A11
OT  - LASP1
OT  - LSCC
OT  - miR-877-5p
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/08/04 06:43
MHDA- 2023/08/04 06:44
PMCR- 2023/07/14
CRDT- 2023/08/04 04:11
PHST- 2023/01/18 00:00 [received]
PHST- 2023/07/10 00:00 [revised]
PHST- 2023/07/13 00:00 [accepted]
PHST- 2023/08/04 06:44 [medline]
PHST- 2023/08/04 06:43 [pubmed]
PHST- 2023/08/04 04:11 [entrez]
PHST- 2023/07/14 00:00 [pmc-release]
AID - S2405-8440(23)05498-1 [pii]
AID - e18290 [pii]
AID - 10.1016/j.heliyon.2023.e18290 [doi]
PST - epublish
SO  - Heliyon. 2023 Jul 14;9(7):e18290. doi: 10.1016/j.heliyon.2023.e18290. eCollection 
      2023 Jul.