PMID- 37541636
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230828
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 140
DP  - 2023 Sep
TI  - Two CcCCL19bs orchestrate an antibacterial immune response in Yellow River carp 
      (Cyprinus carpio haematopterus).
PG  - 108987
LID - S1050-4648(23)00473-4 [pii]
LID - 10.1016/j.fsi.2023.108987 [doi]
AB  - Chemokines are a group of chemotactic cytokines with an essential role in 
      homeostasis as well as immunity via specific G protein-coupled receptors and 
      atypical receptors. In our study, two Yellow River carp (Cyprinus carpio 
      haematopterus) CCL19b genes (CcCCL19bs), tentatively named CcCCL19b_a and 
      CcCCL19b_b, were cloned. The open reading frames (ORFs) of CcCCL19b_a and 
      CcCCL19b_b were both 333 bp that encoded a 12 kDa protein with 110 amino acid 
      residues. CcCCL19bs contained a signal peptide and a SCY domain with four typical 
      conserved cysteine residues. The two CcCCL19b proteins shared high similarities 
      with each other in both secondary and three-dimensional structure. Phylogenetic 
      analysis showed that CcCCL19bs and other CCL19bs from tetraploid cyprinid fish 
      were clustered into one clade. CcCCL19bs were highly expressed in gill and 
      intestine in healthy fish, and a significant up-regulation of gene expression 
      after Aeromonas hydrophila infection and poly(I:C) stimulation was observed in 
      gill, liver, and head kidney. Furthermore, chemotaxis and antibacterial activity 
      of CcCCL19bs were studied. The results indicated that recombinant CcCCL19b_a and 
      CcCCL19b_b protein (rCcCCL19b_a and rCcCCL19b_b) exhibited significant attraction 
      to primary head kidney leukocytes (HKLs). Meanwhile, both of rCcCCL19bs could 
      promote the proliferation of HKLs, and significantly up-regulate the expressions 
      of IL-1beta, CCR7, and IL-6, and down-regulate the expression of IL-10 in primary 
      HKLs. In vitro, rCcCCL19bs could bind and aggregate A. hydrophila and 
      Staphylococcus aureus. The rCcCCL19bs exhibited significant antibacterial 
      activity against A. hydrophila, but not S. aureus. Moreover, they inhibited the 
      growth of A. hydrophila and S. aureus. In vivo, overexpression of CcCCL19bs 
      contributed to the bacterial clearance. These studies suggested that CcCCL19bs 
      orchestrate an antibacterial immune response.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Qiao, Dan
AU  - Qiao D
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Zhao, Yanjing
AU  - Zhao Y
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Pei, Chao
AU  - Pei C
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Zhao, Xianliang
AU  - Zhao X
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Jiang, Xinyu
AU  - Jiang X
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Zhu, Lei
AU  - Zhu L
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Li, Li
AU  - Li L
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China.
FAU - Kong, Xianghui
AU  - Kong X
AD  - Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of 
      Fisheries, Henan Normal University, Henan province, PR China. Electronic address: 
      xhkong@htu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230802
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - O84C90HH2L (Poly I-C)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - Immunity, Innate/genetics
MH  - *Carps/genetics/metabolism
MH  - Phylogeny
MH  - Poly I-C/pharmacology
MH  - Anti-Bacterial Agents
MH  - Aeromonas hydrophila/physiology
MH  - *Fish Diseases
MH  - Fish Proteins/chemistry
MH  - *Gram-Negative Bacterial Infections
OTO - NOTNLM
OT  - Antibacterial defense
OT  - Chemokine CCL19b
OT  - Cyprinus carpio haematopterus
OT  - Innate immunity
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/05 05:42
MHDA- 2023/08/28 06:41
CRDT- 2023/08/04 19:18
PHST- 2023/03/31 00:00 [received]
PHST- 2023/07/23 00:00 [revised]
PHST- 2023/08/02 00:00 [accepted]
PHST- 2023/08/28 06:41 [medline]
PHST- 2023/08/05 05:42 [pubmed]
PHST- 2023/08/04 19:18 [entrez]
AID - S1050-4648(23)00473-4 [pii]
AID - 10.1016/j.fsi.2023.108987 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2023 Sep;140:108987. doi: 10.1016/j.fsi.2023.108987. Epub 
      2023 Aug 2.