PMID- 37543857
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230808
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 25
IP  - 7
DP  - 2023 Jul 25
TI  - Mouse Embryonic Fibroblasts-Derived Extracellular Matrix Facilitates Expansion of 
      Inner Ear-Derived Cells.
PG  - 447-454
LID - 705222 [pii]
LID - 10.22074/cellj.2023.1989426.1228 [doi]
AB  - OBJECTIVE: Previous reports showed that mouse embryonic fibroblasts (MEFs) could 
      support pluripotent stem cell selfrenewal and maintain their pluripotency. The 
      goal of this study was to reveal whether the decellularized extracellular matrix 
      derived from MEFs (MEF-ECM) is beneficial to promote the proliferation of inner 
      ear-derived cells. MATERIALS AND METHODS: In this experimental study, we prepared 
      a cell-free MEF-ECM through decellularization. Scanning electron microscope (SEM) 
      and immunofluorescent staining were conducted for phenotype characterization. 
      Organs of Corti were dissected from postnatal day 2 and the inner ear-derived 
      cells were obtained. The identification of inner ear-derived cells was conducted 
      by using reverse transcription-polymerase chain reaction (RT-PCR). Cell counting 
      kit-8 (CCK-8) was used to evaluate the proliferation capability of inner 
      ear-derived cells cultured on the MEFECM and tissue culture plate (TCP). RESULTS: 
      The MEF-ECM was clearly observed after decellularization via SEM, and the 
      immunofluorescence staining results revealed that MEF-ECM was composed of three 
      proteins, including collagen I, fibronectin and laminin. Most importantly, the 
      results of CCK-8 showed that compared with TCP, MEF-ECM could effectively 
      facilitate the proliferation of inner ear-derived cells. CONCLUSION: The 
      discovery of the potential of MEF-ECM in promoting inner ear-derived cell 
      proliferation indicates that the decellularized matrix microenvironment may play 
      a vital role in keeping proliferation ability of these cells. Our findings 
      indicate that the use of MEF-ECM may serve as a novel approach for expanding 
      inner ear-derived cells and potentially facilitating the clinical application of 
      inner ear-derived cells for hearing loss in the future.
FAU - Zhang, Junming
AU  - Zhang J
AD  - College of Biological Science and Medical Engineering, Donghua University, 
      Shanghai, China.
FAU - Liu, Li
AU  - Liu L
AD  - College of Biological Science and Medical Engineering, Donghua University, 
      Shanghai, China.
FAU - Li, Yuexia
AU  - Li Y
AD  - College of Biological Science and Medical Engineering, Donghua University, 
      Shanghai, China.
FAU - Wu, Jinglei
AU  - Wu J
AD  - College of Biological Science and Medical Engineering, Donghua University, 
      Shanghai, China.
FAU - Lou, Xiangxin
AU  - Lou X
AUID- ORCID: 0000-0002-1673-7715
AD  - College of Biological Science and Medical Engineering, Donghua University, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20230725
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC10404357
OTO - NOTNLM
OT  - Decellularized Extracellular Matrix
OT  - Fibroblasts
OT  - Hearing loss
OT  - Organ of Corti
EDAT- 2023/08/06 13:13
MHDA- 2023/08/06 13:14
PMCR- 2023/07/01
CRDT- 2023/08/06 07:02
PHST- 2023/02/09 00:00 [received]
PHST- 2023/08/06 13:14 [medline]
PHST- 2023/08/06 13:13 [pubmed]
PHST- 2023/08/06 07:02 [entrez]
PHST- 2023/07/01 00:00 [pmc-release]
AID - 705222 [pii]
AID - 10.22074/cellj.2023.1989426.1228 [doi]
PST - epublish
SO  - Cell J. 2023 Jul 25;25(7):447-454. doi: 10.22074/cellj.2023.1989426.1228.