PMID- 37547571
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2228-5482 (Print)
IS  - 2251-676X (Electronic)
IS  - 2228-5482 (Linking)
VI  - 24
IP  - 2
DP  - 2023 Apr-Jun
TI  - Comparison of In-House Microfluidic Device and Centrifuge-Based Method Efficacy 
      in Sperm Preparation for Assisted Reproductive Technology.
PG  - 85-93
LID - 10.18502/jri.v24i2.12492 [doi]
AB  - BACKGROUND: Sperm DNA fragmentation can affect reproductive outcomes in assisted 
      reproductive techniques (ART), and it is a concern in density gradient 
      centrifugation (DGC). By contrast, microfluidic approaches allow the selection of 
      highly motile sperm with low DNA fragmentation index (DFI). The purpose of 
      current study, was to compare the efficacy of a microfluidic device designed 
      in-house in comparison with DGC. METHODS: Nineteen healthy men with normal semen 
      profiles were included in the study. Semen samples were individually aliquoted 
      for three sperm preparation analyses (crude and processed with to either DGC or 
      the microfluidic method). Sperm parameters of the samples were evaluated along 
      with DNA fragmentation using the terminal deoxynucleotidyl transferase dUTP 
      nick-end labeling (TUNEL) method. RESULTS: Sperm processed using the microfluidic 
      method showed a significantly lower DFI than those obtained using DGC and in 
      crude semen, with DFI of 1.1%, 3.5%, and 4.9%, respectively. Although the 
      microfluidic method yielded significantly lower sperm concentrations than DGC, no 
      significant differences were observed in total motility, progressive motility, 
      curvilinear velocity, straight-line velocity, or normal morphology. CONCLUSION: 
      Using the in-house microfluidic device, sperm with lower DFI was effectively 
      isolated when compared with DGC. The motility and normal morphology rates were 
      comparable among the samples.
CI  - Copyright(c) 2023, Avicenna Research Institute.
FAU - Phairatana, Tonghathai
AU  - Phairatana T
AD  - Department of Biomedical Sciences and Biomedical Engineering, Faculty of 
      Medicine, Prince of Songkla University, Songkhla, Thailand.
FAU - Prateepchaikul, Thanaporn
AU  - Prateepchaikul T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla 
      University, Songkhla, Thailand.
FAU - Navakanittworakul, Raphatphorn
AU  - Navakanittworakul R
AD  - Department of Biomedical Sciences and Biomedical Engineering, Faculty of 
      Medicine, Prince of Songkla University, Songkhla, Thailand.
FAU - Choksuchat, Chainarong
AU  - Choksuchat C
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla 
      University, Songkhla, Thailand.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - J Reprod Infertil
JT  - Journal of reproduction & infertility
JID - 101535586
PMC - PMC10402458
OTO - NOTNLM
OT  - Assisted reproductive technology
OT  - DNA fragmentation
OT  - Density gradient centrifugation
OT  - Microfluidics
OT  - Sperm.
COIS- Conflict of Interest The authors declare that they have no competing interests.
EDAT- 2023/08/07 06:42
MHDA- 2023/08/07 06:43
PMCR- 2023/04/01
CRDT- 2023/08/07 04:55
PHST- 2022/10/13 00:00 [received]
PHST- 2023/03/12 00:00 [accepted]
PHST- 2023/08/07 06:43 [medline]
PHST- 2023/08/07 06:42 [pubmed]
PHST- 2023/08/07 04:55 [entrez]
PHST- 2023/04/01 00:00 [pmc-release]
AID - JRI-24-85 [pii]
AID - 10.18502/jri.v24i2.12492 [doi]
PST - ppublish
SO  - J Reprod Infertil. 2023 Apr-Jun;24(2):85-93. doi: 10.18502/jri.v24i2.12492.