PMID- 37551997
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231024
IS  - 2198-3844 (Electronic)
IS  - 2198-3844 (Linking)
VI  - 10
IP  - 29
DP  - 2023 Oct
TI  - The Interaction between Macrophages and Triple-negative Breast Cancer Cells 
      Induces ROS-Mediated Interleukin 1alpha Expression to Enhance Tumorigenesis and 
      Metastasis.
PG  - e2302857
LID - 10.1002/advs.202302857 [doi]
LID - 2302857
AB  - Triple-negative breast cancer (TNBC) has higher mortality than non-TNBC because 
      of its stronger metastatic capacity. Increasing studies reported that TNBC tumors 
      had more macrophage infiltration than non-TNBC tumors, which promoted the 
      metastasis of TNBC cells. However, how TNBC cells become more malignant after 
      interacting with macrophages is less reported. In this study, it is observed that 
      when TNBC cells are co-cultured with macrophages, they display higher viability 
      and stronger metastatic ability than non-TNBC cells. Mechanistic studies reveal 
      that TNBC cells acquired these abilities via interactions with macrophages in 
      three phases. First, within 12 h of co-culture with macrophages, some TNBC cells 
      have significantly elevated levels of reactive oxygen species (ROS), which 
      upregulate interleukin 1alpha (IL1alpha) expression in ERK1/2-c-Jun- and NF-kappaB-dependent 
      manners at 24-48 h. Second, the secreted IL1alpha bound to IL1R1 activates the 
      ERK1/2-ZEB1-VIM pathway which increases metastasis. Third, IL1alpha/IL1R1 facilitates 
      its own synthesis and induces the expression of IL1beta and IL8 at 72-96 h through 
      the MKK4-JNK-c-Jun and NF-kappaB signaling pathways. Moreover, a higher level of IL1alpha 
      is positively correlated with more macrophage infiltration and shorter overall 
      survival in breast cancer patients. Thus, reducing ROS elevation or 
      downregulating IL1alpha expression can serve as new strategies to decrease metastasis 
      of TNBC.
CI  - (c) 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
FAU - Hao, Meng
AU  - Hao M
AUID- ORCID: 0009-0002-0784-9376
AD  - Department of Biomedical Sciences, Faculty of Health Sciences, University of 
      Macau, Taipa, Macao SAR, 99078, China.
FAU - Huang, Bin
AU  - Huang B
AD  - Department of Biomedical Sciences, Faculty of Health Sciences, University of 
      Macau, Taipa, Macao SAR, 99078, China.
FAU - Wu, Renfei
AU  - Wu R
AD  - Department of Biomedical Sciences, Faculty of Health Sciences, University of 
      Macau, Taipa, Macao SAR, 99078, China.
FAU - Peng, Zheng
AU  - Peng Z
AD  - Department of Biomedical Sciences, Faculty of Health Sciences, University of 
      Macau, Taipa, Macao SAR, 99078, China.
FAU - Luo, Kathy Qian
AU  - Luo KQ
AUID- ORCID: 0000-0001-7454-9547
AD  - Department of Biomedical Sciences, Faculty of Health Sciences, University of 
      Macau, Taipa, Macao SAR, 99078, China.
AD  - Ministry of Education Frontiers Science Center for Precision Oncology, University 
      of Macau, Taipa, Macao SAR, 99078, China.
LA  - eng
GR  - Faculty of Health Sciences/
GR  - 068/2017/A2/Science and Technology Development Fund/
GR  - 0147/2020/A3/Science and Technology Development Fund/
GR  - 0004/2021/AKP/Science and Technology Development Fund/
GR  - SP2021-00001-FSCPO/Ministry of Education Frontiers Science Centre for Precision 
      Oncology, University of Macau/
GR  - SP2023-00001-FSCPO/Ministry of Education Frontiers Science Centre for Precision 
      Oncology, University of Macau/
PT  - Journal Article
DEP - 20230808
PL  - Germany
TA  - Adv Sci (Weinh)
JT  - Advanced science (Weinheim, Baden-Wurttemberg, Germany)
JID - 101664569
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Interleukin-1alpha)
SB  - IM
MH  - Humans
MH  - *NF-kappa B/metabolism
MH  - *Triple Negative Breast Neoplasms/pathology
MH  - Reactive Oxygen Species/metabolism
MH  - Interleukin-1alpha/metabolism
MH  - Cell Line, Tumor
MH  - Carcinogenesis/metabolism
MH  - Cell Transformation, Neoplastic
MH  - Macrophages/metabolism
PMC - PMC10582438
OTO - NOTNLM
OT  - co-culture
OT  - interleukin 1alpha (IL1alpha)
OT  - macrophages
OT  - metastasis
OT  - reactive oxygen species (ROS)
OT  - triple-negative breast cancer (TNBC)
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/08 12:41
MHDA- 2023/10/23 12:45
PMCR- 2023/08/08
CRDT- 2023/08/08 09:13
PHST- 2023/07/24 00:00 [revised]
PHST- 2023/05/05 00:00 [received]
PHST- 2023/10/23 12:45 [medline]
PHST- 2023/08/08 12:41 [pubmed]
PHST- 2023/08/08 09:13 [entrez]
PHST- 2023/08/08 00:00 [pmc-release]
AID - ADVS6271 [pii]
AID - 10.1002/advs.202302857 [doi]
PST - ppublish
SO  - Adv Sci (Weinh). 2023 Oct;10(29):e2302857. doi: 10.1002/advs.202302857. Epub 2023 
      Aug 8.