PMID- 37554438 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230810 IS - 2589-0042 (Electronic) IS - 2589-0042 (Linking) VI - 26 IP - 8 DP - 2023 Aug 18 TI - An association study of HLA with levofloxacin-induced severe cutaneous adverse drug reactions in Han Chinese. PG - 107391 LID - 10.1016/j.isci.2023.107391 [doi] LID - 107391 AB - Levofloxacin-induced severe cutaneous adverse drug reactions (LEV-SCARs) remain unexplored. An association study of human leukocyte antigen (HLA) alleles with LEV-SCARs among 12 patients, 806 healthy subjects, and 100 levofloxacin-tolerant individuals was performed. The carrier frequencies of HLA-B *13:01 (odds ratio [OR]: 4.50; 95% confidence interval [CI]: 1.15-17.65; p = 0.043), HLA-B *13:02 (OR: 6.14; 95% CI: 1.73-21.76; p = 0.0072), and serotype B13 (OR: 17.73; 95% CI: 3.61-86.95; p = 4.85 x 10(-5)) in patients with LEV-SCARs were significantly higher than those of levofloxacin-tolerant individuals. Molecular docking analysis suggested that levofloxacin formed more stable binding models with HLA-B *13:01 and HLA-B *13:02 than with non-risk HLA-B *46:01. Mass spectrometry revealed that nonapeptides bound to HLA-B *13:02 shifted at several positions after exposure to levofloxacin. Prospective screening for serotype B13 (sensitivity: 83%, specificity: 78%) and alternative drug treatment for carriers may significantly decrease the incidence of LEV-SCARs. CI - (c) 2023 The Authors. FAU - Jiang, Menglin AU - Jiang M AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. FAU - Yang, Jin AU - Yang J AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Yang, Linlin AU - Yang L AD - Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. FAU - Wang, Lina AU - Wang L AD - Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. FAU - Wang, Ting AU - Wang T AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. FAU - Han, Shengna AU - Han S AD - Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. FAU - Cheng, Ye AU - Cheng Y AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. FAU - Chen, Zihua AU - Chen Z AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Su, Yu AU - Su Y AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. FAU - Zhang, Lirong AU - Zhang L AD - Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. FAU - Yang, Fanping AU - Yang F AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Chen, Sheng-An AU - Chen SA AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Zhang, Jin AU - Zhang J AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. FAU - Xiong, Hao AU - Xiong H AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Wang, Lanting AU - Wang L AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Zhang, Zhen AU - Zhang Z AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Ma, Li AU - Ma L AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Luo, Xiaoqun AU - Luo X AD - Department of Allergy and Immunology, Huashan Hospital, Fudan University, Shanghai 200040, China. AD - Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China. FAU - Xing, Qinghe AU - Xing Q AD - Children's Hospital of Fudan University and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China. LA - eng SI - figshare/10.6084/m9.figshare.23311421 PT - Journal Article DEP - 20230713 PL - United States TA - iScience JT - iScience JID - 101724038 PMC - PMC10404721 OTO - NOTNLM OT - Dermatology OT - Genetics OT - Health sciences OT - Immunology COIS- The authors declare no competing interests. EDAT- 2023/08/09 06:43 MHDA- 2023/08/09 06:44 PMCR- 2023/07/13 CRDT- 2023/08/09 04:00 PHST- 2022/10/21 00:00 [received] PHST- 2023/03/21 00:00 [revised] PHST- 2023/07/10 00:00 [accepted] PHST- 2023/08/09 06:44 [medline] PHST- 2023/08/09 06:43 [pubmed] PHST- 2023/08/09 04:00 [entrez] PHST- 2023/07/13 00:00 [pmc-release] AID - S2589-0042(23)01468-2 [pii] AID - 107391 [pii] AID - 10.1016/j.isci.2023.107391 [doi] PST - epublish SO - iScience. 2023 Jul 13;26(8):107391. doi: 10.1016/j.isci.2023.107391. eCollection 2023 Aug 18.