PMID- 37554943
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230810
IS  - 2189-7255 (Electronic)
IS  - 2189-7247 (Print)
IS  - 2189-7247 (Linking)
VI  - 9
IP  - 3
DP  - 2023 Aug
TI  - Effects of recombinant human soluble thrombomodulin on neutrophil extracellular 
      traps in the kidney of a mouse model of endotoxin shock.
PG  - 225-230
LID - 10.20407/fmj.2022-026 [doi]
AB  - OBJECTIVES: Sepsis is a life-threatening condition characterized by multi-organ 
      dysfunction due to host immune system dysregulation in response to an infection. 
      During sepsis, neutrophils release neutrophil extracellular traps (NETs) as part 
      of the innate immune response. However, excessive NETs play a critical role in 
      the development of organ failure during sepsis. Although recombinant human 
      soluble thrombomodulin (rTM) can inhibit NET formation in the lungs and liver of 
      a mouse model of endotoxin shock, its effects on the kidneys are unclear. 
      METHODS: The specific effects of NETs and rTM on the renal cortex and renal 
      medulla were examined in a mouse model of endotoxin shock generated by 
      intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), followed by i.p. 
      injection of rTM or an identical volume of saline 1 h later. RESULTS: LPS 
      injection increased serum creatinine, blood urea nitrogen, and histone H3 levels. 
      However, rTM administration significantly decreased histone H3 and citrullinated 
      histone H3 (citH3) levels. Immunohistochemical analysis revealed no significant 
      changes in citH3 quantity in the renal cortex of any group. However, in the renal 
      medulla, the increase in citH3 induced by LPS was abolished in the LPS+rTM group. 
      CONCLUSIONS: Our findings demonstrate that rTM can suppress NETs in the renal 
      medulla of mice with endotoxin-induced acute kidney injury.
FAU - Harada, Tatsuhiko
AU  - Harada T
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Shimomura, Yasuyo
AU  - Shimomura Y
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Nishida, Osamu
AU  - Nishida O
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Maeda, Munenori
AU  - Maeda M
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Kato, Yu
AU  - Kato Y
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Nakamura, Tomoyuki
AU  - Nakamura T
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Kuriyama, Naohide
AU  - Kuriyama N
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
FAU - Komura, Hidefumi
AU  - Komura H
AD  - Department of Anesthesiology and Critical Care Medicine, Fujita Health 
      University, School of Medicine, Toyoake, Aichi, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221227
PL  - Japan
TA  - Fujita Med J
JT  - Fujita medical journal
JID - 101745077
PMC - PMC10405902
OTO - NOTNLM
OT  - Kidney
OT  - Lipopolysaccharide
OT  - Neutrophil extracellular trap
OT  - Sepsis
OT  - Thrombomodulin
EDAT- 2023/08/09 06:43
MHDA- 2023/08/09 06:44
PMCR- 2022/12/27
CRDT- 2023/08/09 04:07
PHST- 2022/06/07 00:00 [received]
PHST- 2022/09/12 00:00 [accepted]
PHST- 2023/08/09 06:44 [medline]
PHST- 2023/08/09 06:43 [pubmed]
PHST- 2023/08/09 04:07 [entrez]
PHST- 2022/12/27 00:00 [pmc-release]
AID - 10.20407/fmj.2022-026 [doi]
PST - ppublish
SO  - Fujita Med J. 2023 Aug;9(3):225-230. doi: 10.20407/fmj.2022-026. Epub 2022 Dec 
      27.