PMID- 37555010
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230810
IS  - 2468-0540 (Electronic)
IS  - 2468-0540 (Linking)
VI  - 8
IP  - 4
DP  - 2023 Dec
TI  - microRNA hsa-miR-425-5p and hsa-miR-4523 expressions as biomarkers of active 
      pulmonary tuberculosis, latent tuberculosis infection, and lymph node 
      tuberculosis.
PG  - 527-533
LID - 10.1016/j.ncrna.2023.07.001 [doi]
AB  - BACKGROUND: Studies on miRNA highlight its significance as an immunomarker for 
      several diseases, including tuberculosis. This study aimed to determine the 
      difference between miR-425-5p and miR-4523 expressions in patients with active 
      pulmonary TB (PTB), latent TB infection (LTBI), and lymph node TB (LNTB), whose 
      diagnosis remains challenging. METHODS: This case-control study was performed on 
      blood samples obtained from 23 patients with PTB, 21 with LTBI, 21 with LNTB, and 
      25 healthy controls (HC). miRNA hsa-miR-425-5p and hsa-miR-4523 expression levels 
      were measured by RT-qPCR. Statistical analyses were performed using SPSS version 
      25.0. RESULTS: RT-qPCR showed that hsa-mir-425-5p and hsa-mir-4523 expression 
      levels were significantly different among the four groups (PTB, LTBI, LNTB, and 
      HCs). The hsa-mir-425-5p miRNA expression level in LNTB was higher than that in 
      LTBI (p = 0.003). Meanwhile, the hsa-mir-4523 miRNA expression was downregulated 
      in PTB and LNTB than in LTBI (p < 0.0001 and p = 0.015, respectively). The ROC 
      analysis of a single sample showed that only mir-4523 could discriminate LTBI and 
      HCs, with an AUC of 0.829 (p < 0.001). The ROC curve of each miRNA was further 
      analyzed after logistic regression by adjusting for sex and age. The combination 
      of both miRNAs was also analyzed. The model that analyzed the combination of both 
      miRNAs after adjusting for age had the best performance in differentiating LNTB 
      from LTBI, with an AUC of 0.97 (p < 0.001). CONCLUSION: miRNA hsa-mir-425-5p was 
      upregulated and miRNA hsa-mir-4523 was downregulated in PTB and LNTB than in 
      LTBI.
CI  - (c) 2023 The Authors.
FAU - Massi, Muhammad Nasrum
AU  - Massi MN
AD  - Department of Clinical Microbiology, Faculty of Medicine, Universitas Hasanuddin, 
      Makassar, 90245, South Sulawesi, Indonesia.
AD  - Hasanuddin University Medical Research Center Laboratory, Faculty of Medicine, 
      Universitas Hasanuddin, Makassar, 90245, South Sulawesi, Indonesia.
AD  - Institute Research and Community Services Universitas Hasanuddin, Makassar, 
      90245, South Sulawesi, Indonesia.
FAU - Hidayah, Najdah
AU  - Hidayah N
AD  - Research Center for Vaccine and Drugs, National Research and Innovation Agency 
      (BRIN), Tangerang Selatan, 15314, Banten, Indonesia.
FAU - Handayani, Irda
AU  - Handayani I
AD  - Department of Clinical Pathology, Faculty of Medicine, Universitas Hasanuddin, 
      Makassar, 90245, South Sulawesi, Indonesia.
FAU - Iskandar, Israini Wiyulanda
AU  - Iskandar IW
AD  - Institute Research and Community Services Universitas Hasanuddin, Makassar, 
      90245, South Sulawesi, Indonesia.
FAU - Djannah, Fathul
AU  - Djannah F
AD  - Department of Anatomy Pathology, Faculty of Medicine, Universitas Mataram, 
      Mataram, 83126, Indonesia.
FAU - Angria, Nirmawati
AU  - Angria N
AD  - Department of Biomedical Sciences, Faculty of Health Technology, Megarezky 
      University, Makassar, 90234, South Sulawesi, Indonesia.
FAU - Halik, Handayani
AU  - Halik H
AD  - Hasanuddin University Medical Research Center Laboratory, Faculty of Medicine, 
      Universitas Hasanuddin, Makassar, 90245, South Sulawesi, Indonesia.
AD  - Department of Biomedical Sciences, Faculty of Health Technology, Megarezky 
      University, Makassar, 90234, South Sulawesi, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20230721
PL  - Netherlands
TA  - Noncoding RNA Res
JT  - Non-coding RNA research
JID - 101688191
PMC - PMC10405153
OTO - NOTNLM
OT  - Latent tuberculosis infection
OT  - Lymph node tuberculosis
OT  - Pulmonary tuberculosis
OT  - miR-425-5p
OT  - miR-4523
COIS- All authors have no conflicts of interest to declare.
EDAT- 2023/08/09 06:43
MHDA- 2023/08/09 06:44
PMCR- 2023/07/21
CRDT- 2023/08/09 04:09
PHST- 2023/03/20 00:00 [received]
PHST- 2023/06/25 00:00 [revised]
PHST- 2023/07/14 00:00 [accepted]
PHST- 2023/08/09 06:44 [medline]
PHST- 2023/08/09 06:43 [pubmed]
PHST- 2023/08/09 04:09 [entrez]
PHST- 2023/07/21 00:00 [pmc-release]
AID - S2468-0540(23)00046-X [pii]
AID - 10.1016/j.ncrna.2023.07.001 [doi]
PST - epublish
SO  - Noncoding RNA Res. 2023 Jul 21;8(4):527-533. doi: 10.1016/j.ncrna.2023.07.001. 
      eCollection 2023 Dec.