PMID- 37559681
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230811
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2023
DP  - 2023
TI  - Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells 
      Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in 
      Preeclampsia.
PG  - 7927747
LID - 10.1155/2023/7927747 [doi]
LID - 7927747
AB  - Preeclampsia is a pregnancy disorder characterized by systemic organ damage and 
      high blood pressure. It has been reported that microRNA-195 (miR-195) is 
      associated with preeclampsia. In this study, we discovered the target of miR-195 
      in regulating human extravillous cytotrophoblast-derived transformed cell 
      proliferation and migration. We analyzed the clinicopathological factors of 
      preeclampsia and normal pregnancies. The messenger ribonucleic acid (mRNA) levels 
      of miR-195 and tissue factor pathway inhibitor 2 (TFPI2) were measured in 
      placental tissues derived from normal and preeclampsia patients by real-time 
      polymerase chain reaction (PCR). Human umbilical cord mesenchymal stem cell 
      (hUC-MSC)-derived extracellular vesicles were verified by western blot. 
      HTR8-S/Vneo cell proliferation was evaluated by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and cell migration 
      rate was assessed by the transwell assay. Relative luciferase activities were 
      measured in TFPI2 wild-type (WT) and mutant cells. miR-195 expression was 
      negatively correlated with TFPI2 mRNA levels in preeclampsia patients. 
      Extracellular vesicles derived from hUC-MSCs enhanced HTR8-S/Vneo cell 
      proliferation and migration. In addition, miR-195 isolated from hUC-MSCs enhanced 
      HTR8-S/Vneo cell proliferation and migration by targeting TFPI2. Our findings 
      demonstrate that the upregulation of miR-195 in extracellular vesicles derived 
      from hUC-MSCs promotes HTR8-S/Vneo cell proliferation and migration by targeting 
      TFPI2.
CI  - Copyright (c) 2023 Ying Chen et al.
FAU - Chen, Ying
AU  - Chen Y
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
FAU - Zhou, Chenchen
AU  - Zhou C
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
FAU - Zhao, Xiaobo
AU  - Zhao X
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
FAU - Che, Ronghua
AU  - Che R
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
FAU - Wu, Yuelin
AU  - Wu Y
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
AD  - Shanghai Key Laboratory of Maternal Medicine, Shanghai First Maternity and Infant 
      Hospital, Tongji University School of Medicine, Shanghai 201204, China.
FAU - Wan, Sheng
AU  - Wan S
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
AD  - Shanghai Key Laboratory of Maternal Medicine, Shanghai First Maternity and Infant 
      Hospital, Tongji University School of Medicine, Shanghai 201204, China.
FAU - Pei, Jinda
AU  - Pei J
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
FAU - Yao, Liping
AU  - Yao L
AUID- ORCID: 0000-0001-6213-5339
AD  - Department of Ultrasound, Shanghai First Maternity and Infant Hospital, Tongji 
      University school of Medicine, Shanghai 201204, China.
FAU - Hua, Xiaolin
AU  - Hua X
AUID- ORCID: 0000-0003-1098-5010
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji 
      University School of Medicine, Shanghai 201204, China.
AD  - Shanghai Key Laboratory of Maternal Medicine, Shanghai First Maternity and Infant 
      Hospital, Tongji University School of Medicine, Shanghai 201204, China.
LA  - eng
PT  - Journal Article
DEP - 20230801
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC10409582
COIS- The authors declare that they have no conflicts of interests.
EDAT- 2023/08/10 06:43
MHDA- 2023/08/10 06:44
PMCR- 2023/08/01
CRDT- 2023/08/10 03:57
PHST- 2021/12/08 00:00 [received]
PHST- 2023/05/28 00:00 [revised]
PHST- 2023/05/30 00:00 [accepted]
PHST- 2023/08/10 06:44 [medline]
PHST- 2023/08/10 06:43 [pubmed]
PHST- 2023/08/10 03:57 [entrez]
PHST- 2023/08/01 00:00 [pmc-release]
AID - 10.1155/2023/7927747 [doi]
PST - epublish
SO  - Stem Cells Int. 2023 Aug 1;2023:7927747. doi: 10.1155/2023/7927747. eCollection 
      2023.