PMID- 37560982
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20231102
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 49
IP  - 5
DP  - 2023 Sep-Oct
TI  - miR-34a/SIRT1/HIF-1alpha axis is involved in cardiac angiogenesis of type 2 diabetic 
      rats: The protective effect of sodium butyrate combined with treadmill exercise.
PG  - 1085-1098
LID - 10.1002/biof.1979 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is one of the most common metabolic disorders 
      worldwide. Recent research has indicated that sodium butyrate (NaB) affects 
      glucose metabolism and exercise has an anti-hyperglycemic effect in diabetes. 
      This study aimed to evaluate the effects of NaB and treadmill exercise on heart 
      angiogenesis through the miR-34a/SIRT1/FOXO1-HIF-1alpha pathway. Diabetic animals 
      received NaB (400 mg/kg daily, orally) and treadmill exercise for 6 weeks. The 
      effect of NaB and treadmill exercise, alone or combined, on miR-34a expression, 
      SIRT1, FOXO1, HIF-1alpha levels, and angiogenesis in diabetic heart tissue was 
      measured. Diabetes caused increased miR-34a (p < 0.01) and FOXO1 (p < 0.001) 
      expression levels. Also, SIRT1 (p < 0.001) and HIF-1alpha (not significant) 
      expression levels were reduced in diabetic rats. NaB and treadmill exercise 
      decreased miR-34a (respectively p < 0.05 and not significant) and FOXO1 (both 
      p < 0.001) expression levels and improved SIRT1 (both not significant) and HIF-1alpha 
      (respectively p < 0.01 and p < 0.001) levels. Also, NaB combined with treadmill 
      exercise decreased miR-34a (p < 0.001) and FOXO1 (p < 0.001) expression levels, 
      and elevated SIRT1 (p < 0.05) and HIF-1alpha (p < 0.001) levels in comparison with 
      the diabetic group. NaB and treadmill exercises modulate the expression of 
      miR-34a and the levels of SIRT1, FOXO1, and HIF-1alpha proteins, thus increasing 
      angiogenesis in the heart tissue of diabetic rats. It can be concluded that NaB 
      and treadmill exercise, alone or combined, may be useful in the treatment of 
      diabetes through the miR-34a/SIRT1/FOXO1-HIF-1alpha pathway.
CI  - (c) 2023 International Union of Biochemistry and Molecular Biology.
FAU - Sarlak, Zeynab
AU  - Sarlak Z
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Eidi, Akram
AU  - Eidi A
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Ghorbanzadeh, Vajihe
AU  - Ghorbanzadeh V
AUID- ORCID: 0000-0002-4195-3516
AD  - Cardiovascular Research Center, Shahid Rahimi Hospital, Lorestan University of 
      Medical Sciences, Khorramabad, Iran.
FAU - Moghaddasi, Mehrnoush
AU  - Moghaddasi M
AD  - Razi Herbal Medicines Research Center, School of Medicine, Lorestan University of 
      Medical Sciences, Khorramabad, Iran.
FAU - Mortazavi, Pejman
AU  - Mortazavi P
AD  - Department of Pathobiology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20230810
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (MicroRNAs)
RN  - 107-92-6 (Butyric Acid)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *MicroRNAs/genetics/metabolism
MH  - *Diabetes Mellitus, Experimental/genetics
MH  - Butyric Acid/pharmacology
MH  - Sirtuin 1/genetics/metabolism
MH  - *Diabetes Mellitus, Type 2/genetics
OTO - NOTNLM
OT  - FOXO1
OT  - HIF-1alpha
OT  - SIRT1
OT  - angiogenesis
OT  - miR-34a
OT  - sodium butyrate
OT  - treadmill exercise
OT  - type 2 diabetes mellitus
EDAT- 2023/08/10 12:42
MHDA- 2023/08/10 12:43
CRDT- 2023/08/10 09:33
PHST- 2023/02/19 00:00 [received]
PHST- 2023/05/11 00:00 [accepted]
PHST- 2023/08/10 12:43 [medline]
PHST- 2023/08/10 12:42 [pubmed]
PHST- 2023/08/10 09:33 [entrez]
AID - 10.1002/biof.1979 [doi]
PST - ppublish
SO  - Biofactors. 2023 Sep-Oct;49(5):1085-1098. doi: 10.1002/biof.1979. Epub 2023 Aug 
      10.