PMID- 37562086
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231127
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 102
DP  - 2023 Dec
TI  - Protective effects of Zishen Huoxue recipe against neuronal injury in the 
      neurovascular unit of rats with vascular dementia by interfering with 
      inflammatory cascade-induced pyroptosis.
PG  - 102358
LID - S0143-4179(23)00039-2 [pii]
LID - 10.1016/j.npep.2023.102358 [doi]
AB  - OBJECTIVE: Chinese herbal formulas show considerable therapeutic benefits in 
      dementia. This study specifically explored the protective action of Zishen Huoxue 
      recipe on the neurovascular unit (NVU) of rats with vascular dementia (VD). 
      METHODS: VD rat models were established by permanent bilateral common carotid 
      artery occlusion and treated with Zishen Huoxue recipe. In vitro glucose‑oxygen 
      deprivation (OGD)-injured NVU models were established and treated with miR-124-3p 
      agomir or rat medicated serum. The neurological damage, histopathological 
      changes, and neuronal injury in the rat hippocampus were assessed using Morris 
      water maze test and histological stainings. Expression of miR-124-3p was 
      determined using RT-qPCR. The blood-brain barrier/NVU injury, cell pyroptosis, 
      NLRP3 inflammasome activation, and release of inflammatory factors were analyzed 
      mainly by immunofluorescence analysis, TUNEL staining, Western blot, and ELISA. 
      QS-21 (an NLRP3 activator) was used to verify the role of miR-124-3p/NLRP3. 
      RESULTS: Zishen Huoxue recipe ameliorated the learning/memory deficits, neuronal 
      injury, NVU insults, cell pyroptosis, activation of NLRP3 inflammasome, and 
      extensive secretion of lactate dehydrogenase/IL-1beta/IL-18 in VD rats. miR-124-3p 
      was downregulated in VD rats but upregulated after treatment of this recipe. 
      miR-124-3p overexpression ameliorated NVU insults, reduced cell pyroptosis, 
      lowered NLRP3 inflammasome activation, and suppressed inflammatory responses in 
      OGD-injured NVU models. NLRP3 inflammasome activation partly counteracted the 
      amelioration effect of miR-124-3p on pyroptosis. Zishen Huoxue recipe could 
      upregulate miR-124-3p to suppress pyroptosis and protect NVU function. 
      CONCLUSION: Zishen Huoxue recipe can upregulate miR-124-3p expression to repress 
      the inflammatory cascade-evoked pyroptosis, thereby protecting against neuronal 
      injury in the NVU of VD rats.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Yao, Ting
AU  - Yao T
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Xie, Le
AU  - Xie L
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Xie, Yao
AU  - Xie Y
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Jiang, Jinlin
AU  - Jiang J
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Deng, Qian
AU  - Deng Q
AD  - Teaching and Residency Program Office, Hunan Hospital of Integrated Traditional 
      Chinese and Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 
      410006, Hunan Province, China.
FAU - Wang, Jie
AU  - Wang J
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Liu, Lu
AU  - Liu L
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China.
FAU - Wu, Dahua
AU  - Wu D
AD  - Department of Neurology, Hunan Hospital of Integrated Traditional Chinese and 
      Western Medicine, No. 58 Lushan Road, Yuelu District, Changsha 410006, Hunan 
      Province, China. Electronic address: Dahuawuu@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230626
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN124 microRNA, rat)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Inflammasomes/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Pyroptosis
MH  - *Dementia, Vascular/drug therapy
MH  - *MicroRNAs/metabolism
OTO - NOTNLM
OT  - 0
OT  - Co-culture system
OT  - Inflammatory cascade response
OT  - NLRP3 inflammasome
OT  - Neurovascular unit
OT  - Vascular dementia
OT  - Zishen Huoxue recipe
OT  - miR-124-3p
COIS- Declaration of Competing Interest The authors have no conflicts of interest to 
      declare.
EDAT- 2023/08/10 18:41
MHDA- 2023/11/27 12:43
CRDT- 2023/08/10 18:00
PHST- 2023/03/31 00:00 [received]
PHST- 2023/06/12 00:00 [revised]
PHST- 2023/06/25 00:00 [accepted]
PHST- 2023/11/27 12:43 [medline]
PHST- 2023/08/10 18:41 [pubmed]
PHST- 2023/08/10 18:00 [entrez]
AID - S0143-4179(23)00039-2 [pii]
AID - 10.1016/j.npep.2023.102358 [doi]
PST - ppublish
SO  - Neuropeptides. 2023 Dec;102:102358. doi: 10.1016/j.npep.2023.102358. Epub 2023 
      Jun 26.