PMID- 3756387
OWN - NLM
STAT- MEDLINE
DCOM- 19861030
LR  - 20151119
IS  - 0007-1285 (Print)
IS  - 0007-1285 (Linking)
VI  - 59
IP  - 705
DP  - 1986 Sep
TI  - The importance of radiolabelling human granulocytes with 111In-tropolonate or 
      111In-2-mercaptopyridine-N-oxide in plasma containing acid-citrate-dextrose.
PG  - 907-10
AB  - When blood cells are radiolabelled in plasma, for example with 111In-tropolonate, 
      the plasma always contains an anticoagulant, usually acid-citrate-dextrose (ACD) 
      or heparin. The effect of ACD and heparin on the labelling efficiency (LE) and 
      optimum concentration of ligand required to radiolabel human granulocytes in 
      plasma with 111In-tropolonate or 111In-2-mercaptopyridine-N-oxide (111In-merc) 
      has been measured. The concentrations of ligand (tropolone or merc) required for 
      maximum cell labelling in plasma containing ACD were 4 X 10(-4) M tropolone and 1 
      X 10(-4) M merc, whereas using plasma containing heparin, the optimum 
      concentrations were 10-fold higher, at 4 X 10(-3) M and 1 X 10(-3) M 
      respectively. At the optimum ligand concentrations, the LE for 1 X 10(8) 
      granulocytes labelled in plasma containing ACD was 90% using 111In-tropolonate 
      and 82% using 111In-merc, whereas using plasma containing heparin they were 68% 
      and only 20%, respectively. Addition of ACD to heparinised plasma abolished the 
      necessity for more ligand and increased the LE to the same values as those for 
      plasma containing ACD alone. These results clearly demonstrate that to obtain a 
      high LE using the lowest possible concentrations of tropolone or merc, the 
      granulocytes must be labelled in plasma containing ACD.
FAU - Danpure, H J
AU  - Danpure HJ
FAU - Osman, S
AU  - Osman S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Radiol
JT  - The British journal of radiology
JID - 0373125
RN  - 0 (Organometallic Compounds)
RN  - 0 (Pyridines)
RN  - 0 (Radioisotopes)
RN  - 0 (Thiones)
RN  - 045A6V3VFX (Indium)
RN  - 13838-07-8 (acid citrate dextrose)
RN  - 14283-79-5 (indium tris(tropolonate))
RN  - 2968PHW8QP (Citric Acid)
RN  - 6GK82EC25D (pyrithione)
RN  - 7L6DL16P1T (Tropolone)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - *Citric Acid
MH  - Glucose/*analogs & derivatives
MH  - Granulocytes/*metabolism
MH  - Humans
MH  - *Indium
MH  - Methods
MH  - *Organometallic Compounds
MH  - Pyridines
MH  - *Radioisotopes
MH  - Thiones
MH  - Tropolone/analogs & derivatives
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1259/0007-1285-59-705-907 [doi]
PST - ppublish
SO  - Br J Radiol. 1986 Sep;59(705):907-10. doi: 10.1259/0007-1285-59-705-907.