PMID- 37565483
OWN - NLM
STAT- MEDLINE
DCOM- 20231002
LR  - 20240210
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 13
IP  - 10
DP  - 2023 Sep 30
TI  - An expanded GCaMP reporter toolkit for functional imaging in Caenorhabditis 
      elegans.
LID - 10.1093/g3journal/jkad183 [doi]
LID - jkad183
AB  - In living organisms, changes in calcium flux are integral to many different 
      cellular functions and are especially critical for the activity of neurons and 
      myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools 
      for reporting changes in calcium levels in vivo. In particular, GCaMPs, derived 
      from GFP, are the most widely used GECIs and have become an invaluable toolkit 
      for neurophysiological studies. Recently, new variants of GCaMP, which offer a 
      greater variety of temporal dynamics and improved brightness, have been 
      developed. However, these variants are not readily available to the 
      Caenorhabditis elegans research community. This work reports a set of GCaMP6 and 
      jGCaMP7 reporters optimized for C. elegans studies. Our toolkit provides 
      reporters with improved dynamic range, varied kinetics, and targeted subcellular 
      localizations. Besides optimized routine uses, this set of reporters is also well 
      suited for studies requiring fast imaging speeds and low magnification or 
      low-cost platforms.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of The 
      Genetics Society of America.
FAU - Ding, Jimmy
AU  - Ding J
AD  - Interdisciplinary Bioengineering Graduate Program, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
FAU - Peng, Lucinda
AU  - Peng L
AD  - Interdisciplinary Bioengineering Graduate Program, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
FAU - Moon, Sihoon
AU  - Moon S
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
FAU - Lee, Hyun Jee
AU  - Lee HJ
AUID- ORCID: 0000-0002-1269-0913
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
FAU - Patel, Dhaval S
AU  - Patel DS
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
FAU - Lu, Hang
AU  - Lu H
AD  - Interdisciplinary Bioengineering Graduate Program, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
LA  - eng
GR  - R01 NS096581/NS/NINDS NIH HHS/United States
GR  - R01 AG056436/AG/NIA NIH HHS/United States
GR  - R01 NS115484/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - SY7Q814VUP (Calcium)
SB  - IM
UOF - bioRxiv. 2023 Mar 08;:. PMID: 36945463
MH  - Animals
MH  - *Caenorhabditis elegans/genetics
MH  - *Calcium
MH  - Neurons/physiology
MH  - Signal Transduction
PMC - PMC10542313
OTO - NOTNLM
OT  - Caenorhabditis elegans
OT  - GCaMP
OT  - calcium reporters
OT  - imaging
OT  - mechanosensation
OT  - microfluidics
COIS- Conflicts of interest The authors declare no conflict of interest.
EDAT- 2023/08/11 12:42
MHDA- 2023/10/02 06:42
PMCR- 2023/08/11
CRDT- 2023/08/11 06:41
PHST- 2023/06/10 00:00 [received]
PHST- 2023/07/16 00:00 [accepted]
PHST- 2023/10/02 06:42 [medline]
PHST- 2023/08/11 12:42 [pubmed]
PHST- 2023/08/11 06:41 [entrez]
PHST- 2023/08/11 00:00 [pmc-release]
AID - 7241152 [pii]
AID - jkad183 [pii]
AID - 10.1093/g3journal/jkad183 [doi]
PST - ppublish
SO  - G3 (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183.