PMID- 37567428
OWN - NLM
STAT- MEDLINE
DCOM- 20230918
LR  - 20230918
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 318
IP  - Pt B
DP  - 2024 Jan 10
TI  - Wei-fu-chun tablet halted gastric intestinal metaplasia and dysplasia associated 
      with inflammation by regulating the NF-kappaB pathway.
PG  - 117020
LID - S0378-8741(23)00888-7 [pii]
LID - 10.1016/j.jep.2023.117020 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Chi006Eese herbal medicine Weifuchun Tablets 
      (WFC) approved by the State Food and Drug Administration in 1982 has been widely 
      used in treating a variety of chronic stomach disorders including Chronic 
      atrophic gastritis (CAG) and Gastric precancerous lesions in China clinically. 
      This study aimed to investigate the efficacy and potential mechanism of WFC in 
      treating Gastric intestinal metaplasia (GIM) and Gastric dysplasia (GDys). 
      MATERIALS AND METHODS: Rat GIM and GDys established by 
      N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) combined with hot paste, ethanol 
      injury, and intermittent fasting were intervened by WFC. Body weight, 
      histopathology, pH of gastric acid, pepsin activity, intestinal metaplasia index 
      and inflammation were detected. Rat bone marrow derived macrophages (BMDMs) 
      pretreated with WFC were stimulated by LPS. Inflammatory factors and the nuclear 
      factor-kappa B (NF-kappaB) pathway were assessed. GES-1 cells pretreated by WFC were 
      stimulated by MNNG and TNF-alpha, intestinal metaplasia index, the NF-kappaB pathway and 
      interaction between P65 and CDX2 were detected. RESULTS: WFC improved rat body 
      weight, histopathology, pH value of gastric acid, activity of gastric pepsin, 
      intestinal metaplasia (CDX2), inflammation (IL-1beta, IL-6 and TNF-alpha), macrophage 
      aggregation (CD68) in gastric mucosa in rat GIM and GDys. WFC inhibited 
      inflammation (IL-1beta and TNF-alpha) by inactivating the NF-kappaB pathway. WFC reduced the 
      expression of CDX2 by inhibiting the binding of CDX2 promoter TSS upstream region 
      with p65. CONCLUSION: WFC blocked GIM and GDys associated with inflammation by 
      regulating the NF-kappaB pathway.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Xie, Dong
AU  - Xie D
AD  - Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases (Ministry of 
      Education), Institute of Liver Diseases, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China; Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China.
FAU - Wu, Chao
AU  - Wu C
AD  - Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases (Ministry of 
      Education), Institute of Liver Diseases, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China; Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China.
FAU - Wang, Dan
AU  - Wang D
AD  - Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases (Ministry of 
      Education), Institute of Liver Diseases, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China; Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China.
FAU - Nisma Lena, Bahaji Azami
AU  - Nisma Lena BA
AD  - Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases (Ministry of 
      Education), Institute of Liver Diseases, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China; Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China.
FAU - Liu, Ningning
AU  - Liu N
AD  - Department of Oncology, Shuguang Hospital, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China.
FAU - Ye, Guan
AU  - Ye G
AD  - Central Research Institute, Shanghai Pharmaceuticals Holding Co., Ltd., Shanghai, 
      China. Electronic address: yeg@sphchina.com.
FAU - Sun, Mingyu
AU  - Sun M
AD  - Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases (Ministry of 
      Education), Institute of Liver Diseases, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China; Shanghai University of Traditional 
      Chinese Medicine, Shanghai, 201203, China. Electronic address: 
      mysun248@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20230809
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Rats
MH  - Animals
MH  - NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Methylnitronitrosoguanidine
MH  - Pepsin A/metabolism
MH  - Inflammation/pathology
MH  - *Precancerous Conditions/drug therapy
MH  - Hyperplasia/pathology
MH  - *Stomach Neoplasms/pathology
MH  - Metaplasia/metabolism/pathology
MH  - Gastric Mucosa/pathology
OTO - NOTNLM
OT  - Gastric dysplasia
OT  - Gastric intestinal metaplasia
OT  - Inflammation
OT  - NF-kappaB pathway
OT  - Wei-fu-chun tablet
COIS- Declaration of competing interest No conflict of interest exits in the submission 
      of this manuscript.
EDAT- 2023/08/12 10:42
MHDA- 2023/09/18 12:42
CRDT- 2023/08/11 19:26
PHST- 2023/05/25 00:00 [received]
PHST- 2023/08/06 00:00 [revised]
PHST- 2023/08/08 00:00 [accepted]
PHST- 2023/09/18 12:42 [medline]
PHST- 2023/08/12 10:42 [pubmed]
PHST- 2023/08/11 19:26 [entrez]
AID - S0378-8741(23)00888-7 [pii]
AID - 10.1016/j.jep.2023.117020 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2024 Jan 10;318(Pt B):117020. doi: 10.1016/j.jep.2023.117020. 
      Epub 2023 Aug 9.