PMID- 37569342
OWN - NLM
STAT- MEDLINE
DCOM- 20230814
LR  - 20230815
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 15
DP  - 2023 Jul 26
TI  - Indoxyl Sulfate Administration during Pregnancy Contributes to Renal Injury and 
      Increased Blood-Brain Barrier Permeability.
LID - 10.3390/ijms241511968 [doi]
LID - 11968
AB  - Rates of pregnancy-related acute kidney injury (PR-AKI) have increased in the U.S 
      over the past two decades, but how PR-AKI affects the blood-brain barrier (BBB) 
      is understudied. AKI is associated with increased amounts of uremic toxins, like 
      indoxyl sulfate (I.S), whose chronic administration leads to BBB and cognitive 
      changes. This study's objective was to determine if (1) PR-AKI increases I.S and 
      (2) if administration of I.S during pregnancy elicits renal injury and/or 
      increases BBB permeability. From gestational day (GD) 11 to GD19, Sprague Dawley 
      rats were given either 100 or 200 mg/kg body-weight dose of I.S. PR-AKI was 
      induced on GD18 via 45 min bilateral renal ischemic reperfusion surgery. On GD18, 
      metabolic cage metrics and metabolic waste was collected and on GD19 blood 
      pressure, and BBB permeability (by Evan's Blue infusion) were measured. I.S and 
      creatinine were measured in both urine and circulation, respectively. One-way 
      ANOVA or student t-tests were performed using GraphPad Prism with a p < 0.05 
      significance. I.S and PR-AKI led to oliguria. I.S administration led to increased 
      BBB permeability compared to normal pregnant and PR-AKI animals. These results 
      suggest that I.S administration during pregnancy leads to increased BBB 
      permeability and evidence of renal injury comparable to PR-AKI animals.
FAU - Griffin, Ashley
AU  - Griffin A
AUID- ORCID: 0000-0002-5899-236X
AD  - Program in Neuroscience, University of Mississippi Medical Center, 2500 North 
      State Street, Jackson, MS 39216, USA.
FAU - Berry, Brittany
AU  - Berry B
AD  - Department of Obstetrics and Gynecology, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS 39216, USA.
FAU - Spencer, Shauna-Kay
AU  - Spencer SK
AD  - Department of Pharmacology and Toxicology, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS 39216, USA.
FAU - Bowles, Teylor
AU  - Bowles T
AD  - Department of Pharmacology and Toxicology, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS 39216, USA.
FAU - Wallace, Kedra
AU  - Wallace K
AUID- ORCID: 0000-0003-4511-0046
AD  - Department of Obstetrics and Gynecology, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS 39216, USA.
AD  - Department of Pharmacology and Toxicology, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS 39216, USA.
LA  - eng
GR  - P20 GM121334/GM/NIGMS NIH HHS/United States
GR  - P20GM121334/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20230726
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - N187WK1Y1J (Indican)
SB  - IM
MH  - Rats
MH  - Pregnancy
MH  - Animals
MH  - Female
MH  - *Blood-Brain Barrier/metabolism
MH  - Rats, Sprague-Dawley
MH  - Indican/metabolism
MH  - Kidney/metabolism
MH  - *Acute Kidney Injury
MH  - Permeability
PMC - PMC10419049
OTO - NOTNLM
OT  - acute kidney injury
OT  - blood-brain barrier
OT  - indoxyl sulfate
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/12 10:47
MHDA- 2023/08/14 06:43
PMCR- 2023/07/26
CRDT- 2023/08/12 01:08
PHST- 2023/06/28 00:00 [received]
PHST- 2023/07/18 00:00 [revised]
PHST- 2023/07/25 00:00 [accepted]
PHST- 2023/08/14 06:43 [medline]
PHST- 2023/08/12 10:47 [pubmed]
PHST- 2023/08/12 01:08 [entrez]
PHST- 2023/07/26 00:00 [pmc-release]
AID - ijms241511968 [pii]
AID - ijms-24-11968 [pii]
AID - 10.3390/ijms241511968 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jul 26;24(15):11968. doi: 10.3390/ijms241511968.