PMID- 37569840
OWN - NLM
STAT- MEDLINE
DCOM- 20230814
LR  - 20230814
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 15
DP  - 2023 Aug 5
TI  - Metabolomics of Type 2 Diabetes Mellitus in Sprague Dawley Rats-In Search of 
      Potential Metabolic Biomarkers.
LID - 10.3390/ijms241512467 [doi]
LID - 12467
AB  - Type 2 diabetes mellitus (T2DM) is an expanding global health concern, closely 
      associated with the epidemic of obesity. Individuals with diabetes are at high 
      risk for microvascular and macrovascular complications, which include 
      retinopathy, neuropathy, and cardiovascular comorbidities. Despite the 
      availability of diagnostic tools for T2DM, approximately 30-60% of people with 
      T2DM in developed countries are never diagnosed or detected. Therefore, there is 
      a strong need for a simpler and more reliable technique for the early detection 
      of T2DM. This study aimed to use a non-targeted metabolomic approach to 
      systematically identify novel biomarkers from the serum samples of T2DM-induced 
      Sprague Dawley (SD) rats using a comprehensive two-dimensional gas chromatography 
      coupled with a time-of-flight mass spectrometry (GCxGC-TOF/MS). Fifty-four male 
      Sprague Dawley rats weighing between 160-180 g were randomly assigned into two 
      experimental groups, namely the type 2 diabetes mellitus group (T2DM) (n = 36) 
      and the non-diabetic control group (n = 18). Results from this study showed that 
      the metabolite signature of the diabetic rats was different from that of the 
      non-diabetic control group. The most significantly upregulated metabolic pathway 
      was aminoacyl-t-RNA biosynthesis. Metabolite changes observed between the 
      diabetic and non-diabetic control group was attributed to the increase in amino 
      acids, such as glycine, L-asparagine, and L-serine. Aromatic amino acids, 
      including L-tyrosine, were associated with the risk of future hyperglycemia and 
      overt diabetes. The identified potential biomarkers depicted a good predictive 
      value of more than 0.8. It was concluded from the results that amino acids that 
      were associated with impaired insulin secretion were prospectively related to an 
      increase in glucose levels. Moreover, amino acids that were associated with 
      impaired insulin secretion were prospectively related to an increase in glucose 
      levels.
FAU - Ndlovu, Innocent Siyanda
AU  - Ndlovu IS
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4000, South Africa.
FAU - Tshilwane, Selaelo Ivy
AU  - Tshilwane SI
AUID- ORCID: 0000-0002-3549-4675
AD  - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria 0110, South Africa.
FAU - Vosloo, Andre
AU  - Vosloo A
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4000, South Africa.
FAU - Chaisi, Mamohale
AU  - Chaisi M
AD  - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria 0110, South Africa.
AD  - Foundational Biodiversity Science, South African National Biodiversity Institute, 
      Pretoria 0001, South Africa.
FAU - Mukaratirwa, Samson
AU  - Mukaratirwa S
AUID- ORCID: 0000-0001-6843-1497
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4000, South Africa.
AD  - One Health Center for Zoonoses and Tropical Veterinary Medicine, School of 
      Veterinary Medicine, Ross University, Basseterre KN0101, Saint Kitts and Nevis.
LA  - eng
GR  - 41011-2020./Ross University School of Veterinary Medicine/
GR  - P2020/11/National Research Foundation/
PT  - Journal Article
DEP - 20230805
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Blood Glucose)
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Male
MH  - Rats
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Rats, Sprague-Dawley
MH  - Hypoglycemic Agents/pharmacology
MH  - *Diabetes Mellitus, Experimental/complications
MH  - Blood Glucose/metabolism
MH  - Metabolomics/methods
MH  - Amino Acids
MH  - Biomarkers
PMC - PMC10419637
OTO - NOTNLM
OT  - Sprague Dawley rats
OT  - biomarkers
OT  - metabolic pathways
OT  - metabolomics
OT  - type 2 diabetes
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/12 10:43
MHDA- 2023/08/14 06:41
PMCR- 2023/08/05
CRDT- 2023/08/12 01:12
PHST- 2023/07/15 00:00 [received]
PHST- 2023/07/27 00:00 [revised]
PHST- 2023/07/28 00:00 [accepted]
PHST- 2023/08/14 06:41 [medline]
PHST- 2023/08/12 10:43 [pubmed]
PHST- 2023/08/12 01:12 [entrez]
PHST- 2023/08/05 00:00 [pmc-release]
AID - ijms241512467 [pii]
AID - ijms-24-12467 [pii]
AID - 10.3390/ijms241512467 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Aug 5;24(15):12467. doi: 10.3390/ijms241512467.