PMID- 37570327
OWN - NLM
STAT- Publisher
LR  - 20230814
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 13
IP  - 15
DP  - 2023 Aug 4
TI  - Dietary Zinc Supplemented in Organic Form Affects the Expression of Inflammatory 
      Molecules in Swine Intestine.
LID - 10.3390/ani13152519 [doi]
LID - 2519
AB  - Animals receiving Zinc (Zn) dietary supplementation with organic sources respond 
      better to stress than inorganic Zn sources supplementation. The study aimed to 
      identify the effect of different Zn sources on intestinal epithelial gene 
      expression. In total, 45 pigs (9 per treatment) (77.5 +/- 2.5 kg weight) were fed 
      for 32 days, a corn-soybean meal diet without supplemented Zn (ZnR) or 
      supplemented with 50 and 100 ppm of inorganic ZnCl(2) (Zn50 and Zn100), and amino 
      acid-bound organic Zn sources (LQ50 and LQ100). Gene expression changes form 
      RNA-seq in ileum tissues of ZnR revealed changes associated with Zn 
      insufficiency. Comparing organic with inorganic Zn sources by one-way ANOVA, 
      pro-inflammatory cytokine interleukin 18 (IL18) was downregulated (p = 0.03) and 
      Toll-like receptor 2 (TLR2) upregulated (p = 0.02). To determine the role of 
      epithelial cells in response to dietary Zn, swine intestinal organoids 
      (enteroids) were exposed to Zn restriction, ZnCl(2) or LQ-Zn. In enteroids, ZIP4 
      expression decreased with added Zn compared with Zn-restriction (p = 0.006) but 
      Zn sources did not affect (p > 0.05) IL18 or TLR2 expression. These results 
      suggest that organic Zn may stimulate TLR2 signaling possibly affecting immune 
      response, while decreasing the proinflammatory cytokine IL18 expression in 
      non-epithelial cells of intestinal mucosa.
FAU - Medida, Ramya Lekha
AU  - Medida RL
AUID- ORCID: 0000-0002-1298-126X
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
FAU - Sharma, Ashok Kumar
AU  - Sharma AK
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
FAU - Guo, Yue
AU  - Guo Y
AUID- ORCID: 0000-0001-7363-319X
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
FAU - Johnston, Lee J
AU  - Johnston LJ
AUID- ORCID: 0000-0003-4164-1835
AD  - West Central Research and Outreach Center (WCROC), University of Minnesota, 
      Morris, MN 56267, USA.
FAU - Urriola, Pedro E
AU  - Urriola PE
AUID- ORCID: 0000-0003-4090-6627
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
FAU - Gomez, Andres
AU  - Gomez A
AUID- ORCID: 0000-0002-1174-0368
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
FAU - Saqui-Salces, Milena
AU  - Saqui-Salces M
AUID- ORCID: 0000-0001-5848-0092
AD  - Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA.
LA  - eng
GR  - MIN-16-112/Minnesota Experimental Station (MAES)/
PT  - Journal Article
DEP - 20230804
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC10417787
OTO - NOTNLM
OT  - immune response
OT  - organic zinc
OT  - organoids
OT  - zinc sources
COIS- The authors declare no conflict of interest. The sponsors had no role in the 
      design, execution, interpretation, or writing of the study.
EDAT- 2023/08/12 10:51
MHDA- 2023/08/12 10:51
PMCR- 2023/08/04
CRDT- 2023/08/12 01:15
PHST- 2023/06/06 00:00 [received]
PHST- 2023/07/22 00:00 [revised]
PHST- 2023/08/01 00:00 [accepted]
PHST- 2023/08/12 10:51 [medline]
PHST- 2023/08/12 10:51 [pubmed]
PHST- 2023/08/12 01:15 [entrez]
PHST- 2023/08/04 00:00 [pmc-release]
AID - ani13152519 [pii]
AID - animals-13-02519 [pii]
AID - 10.3390/ani13152519 [doi]
PST - epublish
SO  - Animals (Basel). 2023 Aug 4;13(15):2519. doi: 10.3390/ani13152519.