PMID- 37572537
OWN - NLM
STAT- MEDLINE
DCOM- 20230823
LR  - 20230823
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1706
DP  - 2023 Sep 13
TI  - Ultrasound assisted membrane-assisted solvent extraction for the simultaneous 
      assessment of some drugs involved in drug-facilitated sexual assaults by liquid 
      chromatography-tandem mass spectrometry.
PG  - 464284
LID - S0021-9673(23)00509-5 [pii]
LID - 10.1016/j.chroma.2023.464284 [doi]
AB  - A simple and highly efficient ultrasound assisted membrane-assisted solvent 
      extraction (MASE) pre-treatment method for urine has been developed and validated 
      for the simultaneous determination of twenty-two drugs involved in 
      drug-facilitated sexual assaults (DFSAs) by liquid chromatography-tandem mass 
      spectrometry. MASE was performed with 4.0 mL of urine (pH adjusted at 12), 400 muL 
      of hexane as an organic solvent inside the polypropylene membrane, and 
      ultrasonication (45 kHz, 120 W) for 10 min. A pre-concentration factor of 40 was 
      achieved after evaporation (N(2) stream) and re-dissolution in 100 microL of 
      methanol. Analytes were separated using a Zorbax Eclipse Plus C18 column under 
      gradient elution with aqueous 10 mM NH(4)HCO(3) (pH 8.0) and methanol as mobile 
      phases. Matrix-matched calibrations allowed the assessment of DFSA drugs of quite 
      different octanol-water partition coefficients (K(o/w)), from 1.32 10(1) for 
      pregabalin to 2.45 10(5) for clomipramine (Log P values from 1.12 (pregabalin) to 
      5.39 (clomipramine)). The limit of detection (LOD) was between 0.0075 to 0.37 microg 
      L(-1), with analytical recoveries ranging from 73 to 103%, and relative standard 
      deviations (RSDs) within the 2-20% range. The applicability of the method was 
      demonstrated after analysing urine samples under forensic investigation.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Justo-Vega, Ana
AU  - Justo-Vega A
AD  - Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials 
      iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 
      Santiago de Compostela 15782, Spain.
FAU - Jinadasa, Kamal K
AU  - Jinadasa KK
AD  - Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials 
      iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 
      Santiago de Compostela 15782, Spain.
FAU - Jayasinghe, G D Thilini Madurangika
AU  - Jayasinghe GDTM
AD  - Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials 
      iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 
      Santiago de Compostela 15782, Spain.
FAU - Alvarez-Freire, Ivan
AU  - Alvarez-Freire I
AD  - Forensic Sciences Institute "Luis Concheiro" (INCIFOR), Department of Pathologic 
      Anatomy and Forensic Sciences, Faculty of Medicine, Universidade de Santiago de 
      Compostela, Rua de San Francisco, s/n, Santiago de Compostela 15782, Spain.
FAU - Bermejo, Ana Maria
AU  - Bermejo AM
AD  - Forensic Sciences Institute "Luis Concheiro" (INCIFOR), Department of Pathologic 
      Anatomy and Forensic Sciences, Faculty of Medicine, Universidade de Santiago de 
      Compostela, Rua de San Francisco, s/n, Santiago de Compostela 15782, Spain.
FAU - Bermejo-Barrera, Pilar
AU  - Bermejo-Barrera P
AD  - Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials 
      iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 
      Santiago de Compostela 15782, Spain.
FAU - Moreda-Pineiro, Antonio
AU  - Moreda-Pineiro A
AD  - Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials 
      iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 
      Santiago de Compostela 15782, Spain. Electronic address: antonio.moreda@usc.es.
LA  - eng
PT  - Journal Article
DEP - 20230805
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - Y4S76JWI15 (Methanol)
RN  - NUV44L116D (Clomipramine)
RN  - 55JG375S6M (Pregabalin)
RN  - 0 (Solvents)
SB  - IM
MH  - *Tandem Mass Spectrometry/methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Methanol
MH  - Clomipramine
MH  - Pregabalin
MH  - Chromatography, Liquid
MH  - Solvents/chemistry
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - Drug-facilitated sexual assault
OT  - Membrane assisted solvent extraction
OT  - Urine
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/13 00:44
MHDA- 2023/08/23 06:42
CRDT- 2023/08/12 18:06
PHST- 2023/01/18 00:00 [received]
PHST- 2023/03/24 00:00 [revised]
PHST- 2023/08/04 00:00 [accepted]
PHST- 2023/08/23 06:42 [medline]
PHST- 2023/08/13 00:44 [pubmed]
PHST- 2023/08/12 18:06 [entrez]
AID - S0021-9673(23)00509-5 [pii]
AID - 10.1016/j.chroma.2023.464284 [doi]
PST - ppublish
SO  - J Chromatogr A. 2023 Sep 13;1706:464284. doi: 10.1016/j.chroma.2023.464284. Epub 
      2023 Aug 5.