PMID- 37573456
OWN - NLM
STAT- MEDLINE
DCOM- 20230816
LR  - 20231121
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Aug 12
TI  - Sex-dependent differences in the genomic profile of lingual sensory neurons in 
      naive and tongue-tumor bearing mice.
PG  - 13117
LID - 10.1038/s41598-023-40380-6 [doi]
LID - 13117
AB  - Mechanisms of sex-dependent orofacial pain are widely understudied. A significant 
      gap in knowledge exists about comprehensive regulation of tissue-specific 
      trigeminal sensory neurons in diseased state of both sexes. Using RNA sequencing 
      of FACS sorted retro-labeled sensory neurons innervating tongue tissue, we 
      determined changes in transcriptomic profiles in males and female mice under 
      naive as well as tongue-tumor bearing conditions Our data revealed the following 
      interesting findings: (1) FACS sorting obtained higher number of neurons from 
      female trigeminal ganglia (TG) compared to males; (2) Naive female neurons 
      innervating the tongue expressed immune cell markers such as Csf1R, C1qa and 
      others, that weren't expressed in males. This was validated by 
      Immunohistochemistry. (3) Accordingly, immune cell markers such as Csf1 
      exclusively sensitized TRPV1 responses in female TG neurons. (4) Male neurons 
      were more tightly regulated than female neurons upon tumor growth and very few 
      differentially expressed genes (DEGs) overlapped between the sexes, (5) Male DEGs 
      contained higher number of transcription factors whereas female DEGs contained 
      higher number of enzymes, cytokines and chemokines. Collectively, this is the 
      first study to characterize the effect of sex as well as of tongue-tumor on 
      global gene expression, pathways and molecular function of tongue-innervating 
      sensory neurons.
CI  - (c) 2023. Springer Nature Limited.
FAU - Ibrahim, Tarek
AU  - Ibrahim T
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA.
FAU - Wu, Ping
AU  - Wu P
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA.
FAU - Wang, Li-Ju
AU  - Wang LJ
AD  - Greehey Children's Cancer Institute, University of Texas Health San Antonio, San 
      Antonio, USA.
AD  - Department of Population Health Sciences, University of Texas Health at San 
      Antonio, San Antonio, USA.
FAU - Fang-Mei, Chang
AU  - Fang-Mei C
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA.
FAU - Murillo, Josue
AU  - Murillo J
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA.
FAU - Merlo, Jaclyn
AU  - Merlo J
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA.
FAU - Shein, Sergey S
AU  - Shein SS
AD  - Department of Microbiology, Immunology and Molecular Genetics, University of 
      Texas Health San Antonio, San Antonio, USA.
FAU - Tumanov, Alexei V
AU  - Tumanov AV
AD  - Department of Microbiology, Immunology and Molecular Genetics, University of 
      Texas Health San Antonio, San Antonio, USA.
FAU - Lai, Zhao
AU  - Lai Z
AD  - Greehey Children's Cancer Institute, University of Texas Health San Antonio, San 
      Antonio, USA.
AD  - Department of Molecular Medicine, University of Texas Health at San Antonio, San 
      Antonio, TX, USA.
FAU - Weldon, Korri
AU  - Weldon K
AD  - Greehey Children's Cancer Institute, University of Texas Health San Antonio, San 
      Antonio, USA.
AD  - Department of Molecular Medicine, University of Texas Health at San Antonio, San 
      Antonio, TX, USA.
FAU - Chen, Yidong
AU  - Chen Y
AD  - Greehey Children's Cancer Institute, University of Texas Health San Antonio, San 
      Antonio, USA.
AD  - Department of Population Health Sciences, University of Texas Health at San 
      Antonio, San Antonio, USA.
FAU - Ruparel, Shivani
AU  - Ruparel S
AD  - Department of Endodontics, School of Dentistry, University of Texas Health 
      Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, 
      USA. ruparels@uthscsa.edu.
LA  - eng
GR  - R01 DE027223/DE/NIDCR NIH HHS/United States
GR  - R01 NS112263/NS/NINDS NIH HHS/United States
GR  - S10 OD030311/OD/NIH HHS/United States
GR  - R01 DE029187/DE/NIDCR NIH HHS/United States
GR  - P30 CA054174/CA/NCI NIH HHS/United States
GR  - S10 OD030432/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230812
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
SB  - IM
UOF - bioRxiv. 2023 May 14;:. PMID: 36711730
MH  - Mice
MH  - Male
MH  - Female
MH  - Animals
MH  - *Sensory Receptor Cells
MH  - *Tongue/metabolism
MH  - Trigeminal Ganglion/metabolism
MH  - Sex Characteristics
MH  - Biomarkers/metabolism
MH  - Genomics
PMC - PMC10423281
COIS- The authors declare no competing interests.
EDAT- 2023/08/13 00:43
MHDA- 2023/08/16 06:43
PMCR- 2023/08/12
CRDT- 2023/08/12 23:29
PHST- 2023/05/13 00:00 [received]
PHST- 2023/08/09 00:00 [accepted]
PHST- 2023/08/16 06:43 [medline]
PHST- 2023/08/13 00:43 [pubmed]
PHST- 2023/08/12 23:29 [entrez]
PHST- 2023/08/12 00:00 [pmc-release]
AID - 10.1038/s41598-023-40380-6 [pii]
AID - 40380 [pii]
AID - 10.1038/s41598-023-40380-6 [doi]
PST - epublish
SO  - Sci Rep. 2023 Aug 12;13(1):13117. doi: 10.1038/s41598-023-40380-6.