PMID- 37574561
OWN - NLM
STAT- MEDLINE
DCOM- 20230815
LR  - 20231121
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 56
IP  - 1
DP  - 2023 Aug 13
TI  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate 
      paclitaxel-induced spermatogenesis defects and maintain male fertility.
PG  - 47
LID - 10.1186/s40659-023-00459-w [doi]
LID - 47
AB  - Chemotherapeutic drugs can cause reproductive damage by affecting sperm quality 
      and other aspects of male fertility. Stem cells are thought to alleviate the 
      damage caused by chemotherapy drugs and to play roles in reproductive protection 
      and treatment. This study aimed to explore the effects of human umbilical cord 
      mesenchymal stem cells (hUC-MSCs) on alleviating paclitaxel (PTX)-induced 
      spermatogenesis and male fertility defects. An in vivo PTX-induced mice model was 
      constructed to evaluate the reproductive toxicity and protective roles of 
      hUC-MSCs in male fertility improvement. A 14 day PTX treatment regimen 
      significantly attenuated mice spermatogenesis and sperm quality, including 
      affecting spermatogenesis, reducing sperm counts, and decreasing sperm motility. 
      hUC-MSCs treatment could significantly improve sperm functional indicators. 
      Mating experiments with normal female mice and examination of embryo development 
      at 7.5 days post-coitum (dpc) showed that hUC-MSCs restored male mouse fertility 
      that was reduced by PTX. In IVF experiments, PTX impaired sperm fertility and 
      blastocyst development, but hUC-MSCs treatment rescued these indicators. 
      hUC-MSCs' protective role was also displayed through the increased expression of 
      the fertility-related proteins HSPA2 and HSPA4L in testes with decreased 
      expression in the PTX-treated group. These changes might be related to the 
      PTX-induced decreases in expression of the germ cell proliferation protein PCNA 
      and the meiosis proteins SYCP3, MLH1, and STRA8, which were restored after 
      hUC-MSCs treatment. In the PTX-treated group, the expression of testicular 
      antioxidant proteins SIRT1, NRF2, CAT, SOD1, and PRDX6 was significantly 
      decreased, but hUC-MSCs could maintain these expressions and reverse PTX-related 
      increases in BAX/BCL2 ratios. hUC-MSCs may be a promising agent with antioxidant 
      and anti-apoptosis characteristics that can maintain sperm quality following 
      chemotherapy treatment.
CI  - (c) 2023. Sociedad de Biologia de Chile.
FAU - Zhang, YuSheng
AU  - Zhang Y
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, China.
FAU - Liu, YaNan
AU  - Liu Y
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, China.
FAU - Teng, Zi
AU  - Teng Z
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China.
FAU - Wang, ZeLin
AU  - Wang Z
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China.
FAU - Zhu, Peng
AU  - Zhu P
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China.
FAU - Wang, ZhiXin
AU  - Wang Z
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China.
FAU - Liu, FuJun
AU  - Liu F
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, China. 
      lfjyt@126.com.
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China. lfjyt@126.com.
FAU - Liu, XueXia
AU  - Liu X
AD  - Shandong Stem Cell Engineering Technology Research Center, Affiliated Yantai 
      Yuhuangding Hospital of Qingdao University, Yantai, China. xue6er6@163.com.
LA  - eng
GR  - 81971438/National Natural Science Foundation of China/
GR  - ZR2019MH035/Natural Science Foundation of Shandong Province/
GR  - ZR2020MH075/Natural Science Foundation of Shandong Province/
PT  - Journal Article
DEP - 20230813
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - P88XT4IS4D (Paclitaxel)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Humans
MH  - Male
MH  - Mice
MH  - Female
MH  - Animals
MH  - Paclitaxel/adverse effects/metabolism
MH  - Antioxidants/metabolism
MH  - Umbilical Cord
MH  - Sperm Motility
MH  - Semen
MH  - Spermatogenesis
MH  - *Mesenchymal Stem Cells
MH  - Fertility
MH  - *Mesenchymal Stem Cell Transplantation
PMC - PMC10424423
OTO - NOTNLM
OT  - Male fertility
OT  - Paclitaxel
OT  - Sperm quality
OT  - Spermatogenesis
OT  - Stem cell
COIS- The authors declare no competing financial interests.
EDAT- 2023/08/14 00:43
MHDA- 2023/08/15 06:42
PMCR- 2023/08/13
CRDT- 2023/08/13 23:14
PHST- 2023/03/30 00:00 [received]
PHST- 2023/07/28 00:00 [accepted]
PHST- 2023/08/15 06:42 [medline]
PHST- 2023/08/14 00:43 [pubmed]
PHST- 2023/08/13 23:14 [entrez]
PHST- 2023/08/13 00:00 [pmc-release]
AID - 10.1186/s40659-023-00459-w [pii]
AID - 459 [pii]
AID - 10.1186/s40659-023-00459-w [doi]
PST - epublish
SO  - Biol Res. 2023 Aug 13;56(1):47. doi: 10.1186/s40659-023-00459-w.