PMID- 37579810 OWN - NLM STAT- MEDLINE DCOM- 20231024 LR - 20231024 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 141 DP - 2023 Oct TI - Oral bovine serum albumin administration alleviates inflammatory signals and improves antioxidant capacity and immune response under thioacetamide stress in blunt snout bream fed a high-calorie diet. PG - 108996 LID - S1050-4648(23)00482-5 [pii] LID - 10.1016/j.fsi.2023.108996 [doi] AB - This investigation looks at the impact of oral bovine serum albumin (BSA) on antioxidants, immune responses, and inflammation signals in blunt snout bream fed a high-calorie diet. 480 fish (average weight: 45.84 +/- 0.07 g) were randomly fed a control diet, a high-fat diet (HFD), a high carbohydrate diet (HCD), and a high-energy diet (HED) in six replicates for 12 weeks. After the feeding trial, fish were orally administered with 10% BSA for 10 h, then blood and liver samples from five fish were randomly obtained after 10 h to determine plasma inflammatory markers and inorganic components. Also, the leftover fish were injected with thioacetamide, blood and liver samples were simultaneously obtained at 12, 48, and 96 h, respectively, to determine antioxidant, immune, and inflammatory signals, with survival rates recorded at the same time interval. After 10 h, plasma inflammatory markers such as tumour necrosis factors (TNF-alpha), interleukin 6 (IL6), nitric oxide (NO), Monocyte chemoattractant protein-1(MCP-1), and cortisol were significantly improved in fish fed HCD and HED as compared to the control. After thioacetamide stress, plasma lysozyme (LYM), complement 3, myeloperoxidase (MPO), and alkaline phosphatase activities, as well as immunoglobulin M, levels all increased significantly (P < 0.05) with increasing time with maximum value attained at 96 h, but shows no difference among dietary treatment. Similar results were observed in liver superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and malondialdehyde (MDA) content, but tended to reduce at 96 h. nf-kb, tnf-alpha, and mcp-1 tend to decrease with the minimum value attained at 48 h and gradually decrease with increasing time at 96 h. After 96 h of the thioacetamide (TAA) challenge, the survival rate of blunt snout bream fed with an HFD and HCD was significantly lower (P < 0.05) at 48, and 96 h before the administration of BSA. However, no differences were observed among dietary treatments after the BSA administration. Overall, this study indicated that oral dietary administration of BSA might greatly enhance the antioxidant capability and innate immunity and mitigates inflammation signals after TAA stress in blunt snout bream fed high energy diet. CI - Copyright (c) 2023 Elsevier Ltd. All rights reserved. FAU - Abasubong, Kenneth Prudence AU - Abasubong KP AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Jiang, Guang-Zhen AU - Jiang GZ AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Guo, Hui-Xing AU - Guo HX AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Wang, Xi AU - Wang X AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Huang, Yang-Yang AU - Huang YY AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Dai, Yong-Jun AU - Dai YJ AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Li, Xiang-Fei AU - Li XF AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Dong, Yan-Zou AU - Dong YZ AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. FAU - Gabriel, Ndakalimwe Naftal AU - Gabriel NN AD - Department of Fisheries and Ocean Sciences, Sam Nujoma Campus, University of Namibia, Private Bag 462, Henties Bay, 9000, Namibia. FAU - Liu, Wen-Bin AU - Liu WB AD - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China; National Laboratory of Animal Science, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China. Electronic address: wbliu@njau.edu.cn. LA - eng PT - Journal Article PT - Randomized Controlled Trial, Veterinary DEP - 20230812 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Antioxidants) RN - 27432CM55Q (Serum Albumin, Bovine) RN - 075T165X8M (Thioacetamide) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Animals MH - Animal Feed/analysis MH - Antioxidants MH - *Cypriniformes MH - Diet MH - Diet, High-Fat MH - Immunity, Innate MH - Inflammation/chemically induced/veterinary MH - *Serum Albumin, Bovine MH - Thioacetamide MH - Tumor Necrosis Factor-alpha OTO - NOTNLM OT - Blunt snout bream: calories diet: immunity: inflammation: oral administration OT - Sampling time: stress COIS- Declaration of competing interest The authors declare that there is no conflict of interest. EDAT- 2023/08/15 00:42 MHDA- 2023/10/23 12:45 CRDT- 2023/08/14 19:21 PHST- 2023/04/18 00:00 [received] PHST- 2023/08/07 00:00 [revised] PHST- 2023/08/11 00:00 [accepted] PHST- 2023/10/23 12:45 [medline] PHST- 2023/08/15 00:42 [pubmed] PHST- 2023/08/14 19:21 [entrez] AID - S1050-4648(23)00482-5 [pii] AID - 10.1016/j.fsi.2023.108996 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2023 Oct;141:108996. doi: 10.1016/j.fsi.2023.108996. Epub 2023 Aug 12.