PMID- 37580372
OWN - NLM
STAT- MEDLINE
DCOM- 20231121
LR  - 20231123
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Aug 14
TI  - Eschar dissolution and the immunoregulator effect of keratinase on burn wounds.
PG  - 13238
LID - 10.1038/s41598-023-39765-4 [doi]
LID - 13238
AB  - At present, enzyme debridement preparation has shown a good curative effect on 
      eschar removal of burn wounds. Keratinase has shown great potential in enzymatic 
      debridement because of its good fibrin-degrading ability. In this study, the 
      debridement of keratinase was examined by using a third degree burn wound model 
      in rats. We observed the wound, and keratinase shortened the time of eschar 
      dissolution after debridement. Histopathology and immunofluorescence staining 
      showed that the eschar in the keratinase group became thinner, inflammatory cell 
      infiltration in the wound increased, the fluorescence intensity of the macrophage 
      surface marker CD68 increased, and the CD163/CD86 ratio increased. In bone 
      marrow-derived macrophages (BMDMs), there was no significant difference in the 
      activity of CCK-8 in cells in the keratinase group compared with the control 
      group. The fluorescence intensity of the keratinase group was higher than that of 
      the control group. At 12 h, the cell scratches were obviously closed. The number 
      of migrated Transwell cells increased. Flow cytometry and immunofluorescence 
      analysis showed increased expression of CD206 and Arg-1 and decreased expression 
      of CD86 and iNOS. The gene expression of the Arg-1, iNOS and IL-10 was increased, 
      as shown by qPCR. The secretion of IL-10 was increased and TNF-alpha was decreased, 
      as shown by ELISA. We concluded that keratinase dissolution of eschar not only 
      has a hydrolytic effect on eschar but may also affect immune regulation to 
      enhance the migration and phagocytosis of macrophages, promote the polarization 
      of macrophages, and further enhance the effect of eschar dissolution. Therefore, 
      keratinase may have good prospects for the debridement of burn wounds.
CI  - (c) 2023. Springer Nature Limited.
FAU - Xu, Yan
AU  - Xu Y
AD  - Wuxi Clinical Medical College of Nanjing University of Traditional Chinese 
      Medicine, Wuxi, 214041, China.
AD  - Affiliated Hospital of Jiangnan University, Wuxi, 214041, China.
FAU - Hu, Kai
AU  - Hu K
AD  - Nanjing University of Chinese Medicine, Nanjing, 210023, China.
FAU - Liu, Chenyang
AU  - Liu C
AD  - Affiliated Hospital of Jiangnan University, Wuxi, 214041, China.
FAU - Du, Pan
AU  - Du P
AD  - Jiangnan University, Wuxi, 214122, China.
FAU - Zhou, Feifan
AU  - Zhou F
AD  - Medical College of Nantong University, Nantong, 226000, China.
FAU - Lu, Yichi
AU  - Lu Y
AD  - Jiangnan University, Wuxi, 214122, China.
FAU - Fu, Qiuyan
AU  - Fu Q
AD  - Jiangnan University, Wuxi, 214122, China.
FAU - Xu, Jianmin
AU  - Xu J
AD  - Affiliated Hospital of Jiangnan University, Wuxi, 214041, China. 
      592220975@qq.com.
FAU - Lyu, Guozhong
AU  - Lyu G
AD  - Affiliated Hospital of Jiangnan University, Wuxi, 214041, China. 
      luguozhong@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230814
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 3.4.- (keratinase)
SB  - IM
MH  - Male
MH  - Animals
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Solubility
MH  - *Burns/enzymology/immunology
MH  - Macrophages/immunology
PMC - PMC10425458
COIS- The authors declare no competing interests.
EDAT- 2023/08/15 00:42
MHDA- 2023/08/16 06:43
PMCR- 2023/08/14
CRDT- 2023/08/14 23:19
PHST- 2023/02/01 00:00 [received]
PHST- 2023/07/30 00:00 [accepted]
PHST- 2023/08/16 06:43 [medline]
PHST- 2023/08/15 00:42 [pubmed]
PHST- 2023/08/14 23:19 [entrez]
PHST- 2023/08/14 00:00 [pmc-release]
AID - 10.1038/s41598-023-39765-4 [pii]
AID - 39765 [pii]
AID - 10.1038/s41598-023-39765-4 [doi]
PST - epublish
SO  - Sci Rep. 2023 Aug 14;13(1):13238. doi: 10.1038/s41598-023-39765-4.