PMID- 37580436 OWN - NLM STAT- MEDLINE DCOM- 20230816 LR - 20231121 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 13 IP - 1 DP - 2023 Aug 14 TI - Identification of new cold tolerant Zoysia grass species using high-resolution RGB and multi-spectral imaging. PG - 13209 LID - 10.1038/s41598-023-40128-2 [doi] LID - 13209 AB - Zoysia grass (Zoysia spp.) is the most widely used warm-season turf grass in Korea due to its durability and resistance to environmental stresses. To develop new longer-period greenness cultivars, it is essential to screen germplasm which maintains the greenness at a lower temperature. Conventional methods are time-consuming, laborious, and subjective. Therefore, in this study, we demonstrate an objective and efficient method to screen maintaining longer greenness germplasm using RGB and multispectral images. From August to December, time-series data were acquired and we calculated green cover percentage (GCP), Normalized Difference Vegetation Index (NDVI), Normalized Difference Red Edge Index (NDRE), Soil-adjusted Vegetation Index (SAVI), and Enhanced Vegetation Index (EVI) values of germplasm from RGB and multispectral images by applying vegetation indexs. The result showed significant differences in GCP, NDVI, NDRE, SAVI, and EVI among germplasm (p < 0.05). The GCP, which evaluated the quantity of greenness by counting pixels of the green area from RGB images, exhibited maintenance of greenness over 90% for August and September but, sharply decrease from October. The study found significant differences in GCP and NDVI among germplasm. san208 exhibiting over 90% GCP and high NDVI values during 153 days. In addition, we also conducted assessments using various vegetation indexes, namely NDRE, SAVI, and EVI. san208 exhibited NDRE levels exceeding 3% throughout this period. As for SAVI, it initially started at approximately 38% and gradually decreased to around 4% over the course of these days. Furthermore, for the month of August, it recorded approximately 6%, but experienced a decline from about 9% to 1% between September and October. The complementary use of both indicators could be an efficient method for objectively assessing the greenness of turf both quantitatively and qualitatively. CI - (c) 2023. Springer Nature Limited. FAU - Ku, Ki-Bon AU - Ku KB AD - Department of Plant Resources and Environment, Jeju National University, Jeju, 63243, Republic of Korea. FAU - Mansoor, Sheikh AU - Mansoor S AD - Department of Plant Resources and Environment, Jeju National University, Jeju, 63243, Republic of Korea. FAU - Han, Gyung Deok AU - Han GD AD - Department of Practical Arts Education, Cheongju National University of Education, Cheongju, 28708, Republic of Korea. FAU - Chung, Yong Suk AU - Chung YS AD - Department of Plant Resources and Environment, Jeju National University, Jeju, 63243, Republic of Korea. yschung@jejunu.ac.kr. FAU - Tuan, Thai Thanh AU - Tuan TT AD - Department of Plant Resources and Environment, Jeju National University, Jeju, 63243, Republic of Korea. thaithanhtuan1987@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230814 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Soil) SB - IM MH - *Poaceae MH - Seasons MH - *Soil MH - Temperature MH - Cold Temperature PMC - PMC10425389 COIS- The authors declare no competing interests. EDAT- 2023/08/15 00:42 MHDA- 2023/08/16 06:42 PMCR- 2023/08/14 CRDT- 2023/08/14 23:23 PHST- 2023/05/18 00:00 [received] PHST- 2023/08/04 00:00 [accepted] PHST- 2023/08/16 06:42 [medline] PHST- 2023/08/15 00:42 [pubmed] PHST- 2023/08/14 23:23 [entrez] PHST- 2023/08/14 00:00 [pmc-release] AID - 10.1038/s41598-023-40128-2 [pii] AID - 40128 [pii] AID - 10.1038/s41598-023-40128-2 [doi] PST - epublish SO - Sci Rep. 2023 Aug 14;13(1):13209. doi: 10.1038/s41598-023-40128-2.