PMID- 37582232
OWN - NLM
STAT- MEDLINE
DCOM- 20230912
LR  - 20230912
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 9
IP  - 9
DP  - 2023 Sep 11
TI  - Effect of Structural Elements of Heparin-Mimicking Polymers on Vascular Cell 
      Distribution and Functions: Chemically Homogeneous or Heterogeneous?
PG  - 5304-5311
LID - 10.1021/acsbiomaterials.3c00860 [doi]
AB  - Heparin-mimicking polymers (HMPs) are artificially synthesized alternatives to 
      heparin with comparable regulatory effects on protein adsorption and cell 
      behavior. By introducing two major structural elements of HMPs (sulfonate- and 
      glyco-containing units) to different areas of material surfaces, heterogeneous 
      surfaces patterned with different HMPs and homogeneous surfaces patterned with 
      the same HMPs can be obtained. In this work, heterogeneous HMP-patterned 
      poly(dimethylsiloxane) (PDMS) surfaces with sulfonate-containing polySS (pS) and 
      glyco-containing polyMAG (pM) distributed in circular patterns (with a diameter 
      of 300 mum) were prepared (S-M and M-S). Specifically, pS and pM were distributed 
      inside and outside the circles on S-M, respectively, and exchanged their 
      distribution on M-S. Homogeneous HMP-patterned silicone surfaces (SM-SM) where 
      sulfonate- and glyco-containing poly(SS-co-MAG) (pSM) were distributed uniformly 
      were prepared. Vascular cells showed interestingly different behaviors between 
      chemically homogeneous and heterogeneous surfaces. They tended to grow in the 
      sulfonate-modified area on S-M and M-S and were distributed uniformly on SM-SM. 
      Compared with M-S, S-M showed a better promoting effect on the growth of vascular 
      cells. Among all the samples, SM-SM exhibited the highest proliferation density 
      and an optimum spreading state of vascular cells, as well as the highest human 
      umbilical vein endothelial cell (HUVEC) viability ( approximately 99%) and relatively low human 
      umbilical vein smooth muscle cell (HUVSMC) viability ( approximately 72%). By heterogeneous or 
      homogeneous patterning with different structural elements of HMPs, the modified 
      silicone surfaces spatially guided vascular cell distribution and functions. This 
      strategy provides a new surface engineering approach to the study of cell-HMP 
      interactions.
FAU - Lei, Jiao
AU  - Lei J
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
FAU - Sun, Wei
AU  - Sun W
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
FAU - Sheng, Denghai
AU  - Sheng D
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
FAU - Wang, Sujian
AU  - Wang S
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
FAU - Liu, Xiaoli
AU  - Liu X
AUID- ORCID: 0000-0003-1686-3292
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
FAU - Zhao, Tingting
AU  - Zhao T
AD  - Jiangsu Biosurf Biotech Company Ltd., Building 26, Dongjing Industrial Square, 
      No. 1, Jintian Road, Suzhou Industrial Park, Suzhou 215123, P.R. China.
FAU - Chen, Hong
AU  - Chen H
AUID- ORCID: 0000-0001-7799-4961
AD  - State and Local Joint Engineering Laboratory for Novel Functional Polymeric 
      Materials, College of Chemistry, Chemical Engineering and Materials Science, 
      Soochow University, 199 Ren-Ai Road, Suzhou 215123, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230815
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
RN  - 9005-49-6 (Heparin)
RN  - 0 (Polymers)
RN  - 0 (Silicones)
SB  - IM
MH  - Humans
MH  - *Heparin/pharmacology/chemistry
MH  - *Polymers/pharmacology/chemistry
MH  - Human Umbilical Vein Endothelial Cells
MH  - Silicones/pharmacology
OTO - NOTNLM
OT  - cell-surface interactions
OT  - heparin-mimicking polymers
OT  - heterogeneous patterns
OT  - homogeneous patterns
OT  - vascular cell behavior
EDAT- 2023/08/15 18:42
MHDA- 2023/09/12 06:41
CRDT- 2023/08/15 15:53
PHST- 2023/09/12 06:41 [medline]
PHST- 2023/08/15 18:42 [pubmed]
PHST- 2023/08/15 15:53 [entrez]
AID - 10.1021/acsbiomaterials.3c00860 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2023 Sep 11;9(9):5304-5311. doi: 
      10.1021/acsbiomaterials.3c00860. Epub 2023 Aug 15.