PMID- 37584525
OWN - NLM
STAT- MEDLINE
DCOM- 20230913
LR  - 20231014
IS  - 2146-3131 (Electronic)
IS  - 2146-3123 (Print)
IS  - 2146-3123 (Linking)
VI  - 40
IP  - 5
DP  - 2023 Sep 11
TI  - HLA Alleles, Genotype and Haplotype Analyzes from Central Anatolia Region of 
      Turkey.
PG  - 358-366
LID - 10.4274/balkanmedj.galenos.2023.2023-4-55 [doi]
AB  - BACKGROUND: Although human leukocyte antigen (HLA) data for the Turkish 
      population has been reported, there are no statistics on the HLA-DPB1 locus, 
      which has recently received significant attention, particularly in hematopoietic 
      stem cell transplantation. In addition, there is no study that has reported the 
      2-6 loci HLA haplotype distribution, 8-digit HLA allele frequency, and genotype 
      frequency in the Turkish population. AIMS: To evaluate the low and high 
      resolution (2-4-8 digits) HLA-A, -B, -C, -DRB1, -DQB1, -DPB1 allele data using 
      the data of 6100 healthy individuals from the Central Anatolian region of Turkey. 
      STUDY DESIGN: Retrospective cross-sectional study. METHODS: All tests were 
      performed using molecular HLA techniques: low-resolution DNA-based 
      sequence-specific oligonucleotides, low/high-resolution DNA-based 
      sequence-specific primer, and high-resolution next generation sequencing. A total 
      of 6100 healthy donors with a minimum of 3 loci (HLA-A, -B, -DRB1) were analyzed 
      for their HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1 data. Pypop and HLA-net 
      GENE[RATE] were used to analyze the data. RESULTS: Among the HLA class I alleles, 
      the following were the most frequently observed alleles: for HLA-A, A*02, A*24, 
      A*03, and A*01; for HLA-B, B*35, B*51, and B*44; and for HLA-C, C*07, C*04, and 
      C*12. Among the HLA class II alleles, the following alelles were the most 
      frequently observed: for HLA-DRB1, DRB1*11, DRB1*04, and DRB1*13; for HLA-DQB1, 
      DQB1*03, DQB1*05, and DQB1*06; and for HLA- DPB1, DPB1*04, DPB1*02, and DPB1*03. 
      The most common alleles among HLA-DPB1 in the 4-digit evaluation were DPB1*04:01, 
      DPB1*02:01, and DPB1*04:02. Among the HLA classes I and II, the following were 
      the most frequently observed 8-digit alleles in HLA-A, -B, -C, -DRB1, -DQB1 and 
      -DPB1 were A*02:01:01:01, B*49:01:01:01, C*04:01:01:06, DRB1*07:01:01:01, 
      DQB1*03:01:01:02, and DPB1*02:01:02:05, respectively. The most common 6 loci 
      haplotype was A*02~B*35~C*04~DRB1*11~DQB1*03~DPB1*04 (2.71%). CONCLUSION: In this 
      study, low and high resolution HLA-DPB1 allele frequency, 6 locus haplotype 
      frequency and genotype frequency were reported for the first time in Turkish 
      population. These new data can be used to map HLA in our country and may provide 
      ideas for potential future studies.
FAU - Yantir, Emel
AU  - Yantir E
AUID- ORCID: 0000-0002-4965-8730
AD  - Department of Immunology, Eskisehir Osmangazi University Faculty of Medicine, 
      Eskisehir, Turkey
FAU - Gunduz, Eren
AU  - Gunduz E
AUID- ORCID: 0000-0001-7455-2949
AD  - Department of Internal Medicine, Division of Hematology, Eskisehir Osmangazi 
      University Faculty of Medicine, Eskisehir, Turkey
FAU - Colak, Ertugrul
AU  - Colak E
AUID- ORCID: 0000-0003-3251-1043
AD  - Department of Biostatistics, Eskisehir Osmangazi University Faculty of Medicine, 
      Eskisehir, Turkey
LA  - eng
PT  - Journal Article
DEP - 20230816
PL  - Turkey
TA  - Balkan Med J
JT  - Balkan medical journal
JID - 101571817
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-A Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Humans
MH  - Haplotypes
MH  - Turkey
MH  - Alleles
MH  - Gene Frequency/genetics
MH  - Retrospective Studies
MH  - Cross-Sectional Studies
MH  - Genotype
MH  - *Histocompatibility Antigens Class I/genetics
MH  - HLA-DRB1 Chains/genetics
MH  - *HLA-A Antigens/genetics
MH  - DNA
PMC - PMC10500136
COIS- Conflict of Interest: No conflict of interest was declared by the authors. 
      Funding: The authors declared that this study received no financial support.
EDAT- 2023/08/16 12:43
MHDA- 2023/09/13 06:42
PMCR- 2023/09/11
CRDT- 2023/08/16 09:34
PHST- 2023/09/13 06:42 [medline]
PHST- 2023/08/16 12:43 [pubmed]
PHST- 2023/08/16 09:34 [entrez]
PHST- 2023/09/11 00:00 [pmc-release]
AID - 62064 [pii]
AID - 10.4274/balkanmedj.galenos.2023.2023-4-55 [doi]
PST - ppublish
SO  - Balkan Med J. 2023 Sep 11;40(5):358-366. doi: 
      10.4274/balkanmedj.galenos.2023.2023-4-55. Epub 2023 Aug 16.