PMID- 37585522
OWN - NLM
STAT- MEDLINE
DCOM- 20230818
LR  - 20230921
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 9
IP  - 33
DP  - 2023 Aug 18
TI  - Profiling single cancer cell metabolism via high-content SRS imaging with 
      chemical sparsity.
PG  - eadg6061
LID - 10.1126/sciadv.adg6061 [doi]
LID - eadg6061
AB  - Metabolic reprogramming in a subpopulation of cancer cells is a hallmark of tumor 
      chemoresistance. However, single-cell metabolic profiling is difficult because of 
      the lack of a method that can simultaneously detect multiple metabolites at the 
      single-cell level. In this study, through hyperspectral stimulated Raman 
      scattering (hSRS) imaging in the carbon-hydrogen (C-H) window and sparsity-driven 
      hyperspectral image decomposition, we demonstrate a high-content hSRS (h(2)SRS) 
      imaging approach that enables the simultaneous mapping of five major 
      biomolecules, including proteins, carbohydrates, fatty acids, cholesterol, and 
      nucleic acids at the single-cell level. h(2)SRS imaging of brain and pancreatic 
      cancer cells under chemotherapy revealed acute and adapted chemotherapy-induced 
      metabolic reprogramming and the unique metabolic features of chemoresistance. Our 
      approach is expected to facilitate the discovery of therapeutic targets to combat 
      chemoresistance. This study illustrates a high-content, label-free chemical 
      imaging approach that measures metabolic profiles at the single-cell level and 
      warrants further research on cellular metabolism.
FAU - Tan, Yuying
AU  - Tan Y
AUID- ORCID: 0000-0003-2536-4778
AD  - Biomedical Engineering, Boston University, Boston, MA 02155, USA.
FAU - Lin, Haonan
AU  - Lin H
AUID- ORCID: 0000-0003-0437-5902
AD  - Biomedical Engineering, Boston University, Boston, MA 02155, USA.
FAU - Cheng, Ji-Xin
AU  - Cheng JX
AUID- ORCID: 0000-0002-5607-6683
AD  - Biomedical Engineering, Boston University, Boston, MA 02155, USA.
AD  - Electrical and Computer Engineering, Boston University, Boston, MA 02155, USA.
AD  - Photonics Center, Boston University, Boston, MA 02155, USA.
LA  - eng
PT  - Journal Article
DEP - 20230816
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Fatty Acids)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - 0 (Proteins)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Humans
MH  - *Neoplasms/drug therapy
MH  - Fatty Acids
MH  - Hydrogen
MH  - Proteins
MH  - Carbon
MH  - Spectrum Analysis, Raman/methods
PMC - PMC10431717
EDAT- 2023/08/16 18:42
MHDA- 2023/08/18 06:42
PMCR- 2023/08/16
CRDT- 2023/08/16 14:04
PHST- 2023/08/18 06:42 [medline]
PHST- 2023/08/16 18:42 [pubmed]
PHST- 2023/08/16 14:04 [entrez]
PHST- 2023/08/16 00:00 [pmc-release]
AID - adg6061 [pii]
AID - 10.1126/sciadv.adg6061 [doi]
PST - ppublish
SO  - Sci Adv. 2023 Aug 18;9(33):eadg6061. doi: 10.1126/sciadv.adg6061. Epub 2023 Aug 
      16.