PMID- 37585718
OWN - NLM
STAT- MEDLINE
DCOM- 20240304
LR  - 20240304
IS  - 1532-2297 (Electronic)
IS  - 1082-6068 (Linking)
VI  - 54
IP  - 3
DP  - 2024
TI  - Effects of astaxanthin on expression of apoptosis and oxidative stress related 
      genes in H(2)O(2) induced oxidative stress BE(2)-C human neuroblastoma cell line.
PG  - 374-381
LID - 10.1080/10826068.2023.2243506 [doi]
AB  - Antioxidants may affect the apoptosis induced by oxidative stress experimental 
      models. The present study was conducted to investigate the effects of astaxanthin 
      on expression of apoptosis and oxidative stress-related genes in H(2)O(2) induced 
      oxidative stress BE(2)-C human neuroblastoma cell line. This experimental study 
      consisted of six groups including control, H(2)O(2) induced oxidative stress 
      control, 100 mM vitamin C intervention, 25 muM astaxanthin intervention (Ax1), 
      50 muM astaxanthin intervention (Ax2) and 100 muM astaxanthin intervention (Ax3). 
      Real-time PCR was used to study the expression of BAX, BCL2, Caspase3 (CAS3), 
      P53, peroxisome proliferator-activated receptor gamma (PPARgamma), superoxide dismutase 
      (SOD), glutathione peroxidase 1 (GPX), catalase (CAT) and nuclear factor 
      erythroid 2-related factor 2 (NRF2). According to the results, among the 
      apoptosis-related genes, CAS3 was down-regulated in groups vitamin C, Ax1 and Ax2 
      compared with H(2)O(2) group, while P53 was down-regulated only in group vitamin 
      C (P < 0.05). Among the oxidative stress-related genes, GPX was up-regulated in 
      groups Ax1, Ax2 and Ax3 compared with H(2)O(2) group, while all the experimental 
      groups showed up-regulation for CAT and NRF2 (P < 0.05). In conclusion, 
      astaxanthin as a powerful antioxidant could inhibit apoptosis via amelioration of 
      CAS3 gene which might be through amelioration of some antioxidant-related genes.
FAU - Hormozi, Maryam
AU  - Hormozi M
AD  - Razi Herbal Medicines Research Center, School of Medicine, Lorestan University of 
      Medical Sciences, Khorramabad, Iran.
FAU - Beigi Boroujeni, Nasim
AU  - Beigi Boroujeni N
AD  - Razi Herbal Medicines Research Center, School of Medicine, Lorestan University of 
      Medical Sciences, Khorramabad, Iran.
FAU - Beigi Boroujeni, Mandana
AU  - Beigi Boroujeni M
AD  - Razi Herbal Medicines Research Center, School of Medicine, Lorestan University of 
      Medical Sciences, Khorramabad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20230810
PL  - England
TA  - Prep Biochem Biotechnol
JT  - Preparative biochemistry & biotechnology
JID - 9607037
RN  - 0 (Antioxidants)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - 8XPW32PR7I (astaxanthine)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - 0 (CRISPR-Associated Proteins)
RN  - 0 (Xanthophylls)
SB  - IM
MH  - Humans
MH  - Antioxidants/pharmacology
MH  - Hydrogen Peroxide/pharmacology
MH  - NF-E2-Related Factor 2/genetics
MH  - Tumor Suppressor Protein p53
MH  - Oxidative Stress
MH  - Ascorbic Acid
MH  - *Neuroblastoma/drug therapy/genetics
MH  - Apoptosis
MH  - Cell Line
MH  - *CRISPR-Associated Proteins
MH  - Xanthophylls
OTO - NOTNLM
OT  - Antioxidants
OT  - apoptosis
OT  - oxidative stress
OT  - systems biology
EDAT- 2023/08/16 18:42
MHDA- 2024/03/04 06:44
CRDT- 2023/08/16 15:33
PHST- 2024/03/04 06:44 [medline]
PHST- 2023/08/16 18:42 [pubmed]
PHST- 2023/08/16 15:33 [entrez]
AID - 10.1080/10826068.2023.2243506 [doi]
PST - ppublish
SO  - Prep Biochem Biotechnol. 2024;54(3):374-381. doi: 10.1080/10826068.2023.2243506. 
      Epub 2023 Aug 10.