PMID- 37590952
OWN - NLM
STAT- MEDLINE
DCOM- 20240104
LR  - 20240104
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 194
IP  - 1
DP  - 2023 Dec 30
TI  - Berberine bridge enzyme-like oxidases of cellodextrins and mixed-linked beta-glucans 
      control seed coat formation.
PG  - 296-313
LID - 10.1093/plphys/kiad457 [doi]
AB  - Plants have evolved various resistance mechanisms to cope with biotic stresses 
      that threaten their survival. The BBE23 member (At5g44360/BBE23) of the 
      Arabidopsis berberine bridge enzyme-like (BBE-l) protein family (Arabidopsis 
      thaliana) has been characterized in this paper in parallel with the closely 
      related and previously described CELLOX (At4g20860/BBE22). In addition to 
      cellodextrins, both enzymes, renamed here as CELLODEXTRIN OXIDASE 2 and 1 
      (CELLOX2 and CELLOX1), respectively, oxidize the mixed-linked beta-1-->3/beta-1-->4-glucans 
      (MLGs), recently described as capable of activating plant immunity, reinforcing 
      the view that the BBE-l family includes members that are devoted to the control 
      of the homeostasis of potential cell wall-derived damage-associated molecular 
      patterns (DAMPs). The 2 putatively paralogous genes display different expression 
      profiles. Unlike CELLOX1, CELLOX2 is not expressed in seedlings or adult plants 
      and is not involved in immunity against Botrytis cinerea. Both are instead 
      expressed in a concerted manner in the seed coat during development. Whereas 
      CELLOX2 is expressed mainly during the heart stage, CELLOX1 is expressed at the 
      immediately later stage, when the expression of CELLOX2 decreases. Analysis of 
      seeds of cellox1 and cellox2 knockout mutants shows alterations in the coat 
      structure: the columella area is smaller in cellox1, radial cell walls are 
      thicker in both cellox1 and cellox2, and the mucilage halo is reduced in cellox2. 
      However, the coat monosaccharide composition is not significantly altered, 
      suggesting an alteration of the organization of the cell wall, thus reinforcing 
      the notion that the architecture of the cell wall in specific organs is 
      determined not only by the dynamics of the synthesis/degradation of the main 
      polysaccharides but also by its enzymatic oxidation.
CI  - (c) American Society of Plant Biologists 2023. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Costantini, Sara
AU  - Costantini S
AUID- ORCID: 0000-0002-6977-9601
AD  - Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 
      00185 Rome, Italy.
FAU - Benedetti, Manuel
AU  - Benedetti M
AUID- ORCID: 0000-0003-0124-4464
AD  - Department of Life, Health and Environmental Sciences, University of L'Aquila, 
      67100 L'Aquila, Italy.
FAU - Pontiggia, Daniela
AU  - Pontiggia D
AUID- ORCID: 0000-0002-0980-107X
AD  - Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 
      00185 Rome, Italy.
AD  - Research Center for Applied Sciences to the Safeguard of Environment and Cultural 
      Heritage (CIABC), Sapienza University of Rome, 00185 Rome, Italy.
FAU - Giovannoni, Moira
AU  - Giovannoni M
AUID- ORCID: 0000-0002-2086-7950
AD  - Department of Life, Health and Environmental Sciences, University of L'Aquila, 
      67100 L'Aquila, Italy.
FAU - Cervone, Felice
AU  - Cervone F
AUID- ORCID: 0000-0001-8141-5352
AD  - Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 
      00185 Rome, Italy.
FAU - Mattei, Benedetta
AU  - Mattei B
AUID- ORCID: 0000-0003-2927-6329
AD  - Department of Life, Health and Environmental Sciences, University of L'Aquila, 
      67100 L'Aquila, Italy.
FAU - De Lorenzo, Giulia
AU  - De Lorenzo G
AUID- ORCID: 0000-0002-1707-5418
AD  - Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 
      00185 Rome, Italy.
LA  - eng
GR  - 2017ZBBYNC/Italian Ministry of University and Research/
GR  - GRANDI_PROGETTI_2021/Sapienza University of Rome/
GR  - AR11816427DBCAD7/Progetti per Avvio alla Ricerca/
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 9061-30-7 (cellodextrin)
RN  - 0 (Arabidopsis Proteins)
RN  - EC 1.21.3.3 (reticuline oxidase)
RN  - EC 1.- (Oxidoreductases)
RN  - 0 (beta-Glucans)
RN  - 0 (Polysaccharides)
RN  - 0 (Plant Mucilage)
SB  - IM
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - Oxidoreductases/metabolism
MH  - *beta-Glucans/metabolism
MH  - *Arabidopsis/metabolism
MH  - Polysaccharides/metabolism
MH  - Seeds/metabolism
MH  - Cell Wall/metabolism
MH  - *Plant Mucilage/metabolism
COIS- Conflict of interest statement. The authors declare no conflict of interest.
EDAT- 2023/08/17 18:42
MHDA- 2024/01/04 11:43
CRDT- 2023/08/17 17:25
PHST- 2023/06/29 00:00 [received]
PHST- 2023/07/27 00:00 [accepted]
PHST- 2024/01/04 11:43 [medline]
PHST- 2023/08/17 18:42 [pubmed]
PHST- 2023/08/17 17:25 [entrez]
AID - 7244651 [pii]
AID - 10.1093/plphys/kiad457 [doi]
PST - ppublish
SO  - Plant Physiol. 2023 Dec 30;194(1):296-313. doi: 10.1093/plphys/kiad457.