PMID- 37597213
OWN - NLM
STAT- MEDLINE
DCOM- 20240112
LR  - 20240202
IS  - 2831-090X (Electronic)
IS  - 2831-0896 (Print)
IS  - 2831-0896 (Linking)
VI  - 24
IP  - 1
DP  - 2024 Jan 3
TI  - Screening and validation of differentially expressed genes in adipose tissue of 
      patients with obesity and type 2 diabetes mellitus.
PG  - 40-50
LID - 10.17305/bb.2023.9498 [doi]
AB  - White adipose tissue (WAT) plays a pivotal role in the onset of type 2 diabetes 
      mellitus (T2DM) and obesity. Despite its significance the underlying pathogenesis 
      and key genes associated with it remain elusive. In our study, we screened the 
      differentially expressed genes (DEGs) in intra-abdominal WAT of T2DM patients 
      with obesity, as well as those with simple obesity, aiming to lay a foundational 
      theory for an in-depth investigation of T2DM pathogenesis and the identification 
      of novel therapeutic targets. Gene expression datasets (GSE16415 and GSE71416) 
      were retrieved from the Gene Expression Omnibus (GEO) database. We employed R for 
      screening DEGs and conducted a functional enrichment analysis using the Metascape 
      database. Combined Lasso regression and Boruta feature selection algorithms were 
      used to identify key DEGs. Subsequently, these were cross-verified using the 
      GSE29231 dataset. Samples and medical records were collected from clinical study 
      participants. The mRNA and protein expressions of the key DEGs were verified 
      using qRT-PCR and western blotting, respectively. We discerned a total of 130 
      DEGs, with 40 being upregulated and 90 downregulated. Functional and pathway 
      enrichment analyses illuminated that these genes are instrumental in mediating 
      metabolite and energy production, neutrophil-mediated immunity, and other 
      associated biological processes. This includes their involvement in the 
      tricarboxylic acid cycle, glycolysis/gluconeogenesis, peroxisome 
      proliferator-activated receptors, and other signalling pathways. Two genes, CIDEA 
      and FSCN1 emerged as key DEGs. The low expression of CIDEA and high expression of 
      FSCN1 in the T2DM and obesity group were verified in clinical samples (P < 0.05). 
      We established that CIDEA and FSCN1 manifest significant differential expression 
      in T2DM patients who are obese. This suggests their potential as risk assessment 
      markers and therapeutic targets for T2DM.
FAU - Tong, Xuewei
AU  - Tong X
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China; Clinical Laboratory Center, People's Hospital of 
      Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.
FAU - Liu, Chunyan
AU  - Liu C
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China.
FAU - Liang, Mengjie
AU  - Liang M
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China.
FAU - Ye, Xueyan
AU  - Ye X
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China; Prenatal Diagnosis Center, Urumqi Maternal and 
      Child Health Hospital, Urumqi, Xinjiang, China.
FAU - Deng, Zhaohui
AU  - Deng Z
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Shihezi 
      University School of Medicine/Hospital of Xinjiang Production and Construction 
      Corps, Urumqi, Xinjiang, China.
LA  - eng
PT  - Journal Article
DEP - 20240103
PL  - Bosnia and Herzegovina
TA  - Biomol Biomed
JT  - Biomolecules & biomedicine
JID - 9918522188506676
RN  - 0 (FSCN1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Microfilament Proteins)
SB  - IM
MH  - Humans
MH  - *Diabetes Mellitus, Type 2/genetics
MH  - Adipose Tissue/metabolism
MH  - Obesity/genetics
MH  - Signal Transduction
MH  - Carrier Proteins/metabolism
MH  - Microfilament Proteins/metabolism
PMC - PMC10787618
COIS- Conflicts of interest: Authors declare no conflicts of interest.
EDAT- 2023/08/19 20:42
MHDA- 2024/01/12 06:42
PMCR- 2024/02/01
CRDT- 2023/08/19 12:52
PHST- 2023/07/11 00:00 [received]
PHST- 2023/08/01 00:00 [accepted]
PHST- 2024/01/12 06:42 [medline]
PHST- 2023/08/19 20:42 [pubmed]
PHST- 2023/08/19 12:52 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - BB-24-40 [pii]
AID - 10.17305/bb.2023.9498 [doi]
PST - epublish
SO  - Biomol Biomed. 2024 Jan 3;24(1):40-50. doi: 10.17305/bb.2023.9498.