PMID- 37602504 OWN - NLM STAT- MEDLINE DCOM- 20240116 LR - 20240116 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) VI - 13 IP - 2 DP - 2024 Jan TI - Fn-HMGB1 Adsorption Behavior Initiates Early Immune Recognition and Subsequent Osteoinduction of Biomaterials. PG - e2301808 LID - 10.1002/adhm.202301808 [doi] AB - Implantable biomaterials are widely used in bone tissue engineering, but little is still known about how they initiate early immune recognition and the initial dynamics. Herein, the early immune recognition and subsequent osteoinduction of biphasic calcium phosphate (BCP) after implantation to the protein adsorption behavior is attributed. By liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis, the biomaterial-related molecular patterns (BAMPs) formed after BCP implantation are mapped, dominated by the highly expressed extracellular matrix protein fibronectin (Fn) and the high mobility group box 1 (HMGB1). Molecular dynamics simulations show that Fn has the ability to bind more readily to the BCP surface than HMGB1. The preferential binding of Fn provides a higher adsorption energy for HMGB1. Furthermore, multiple hydrogen bonding sites between HMGB1 and Fn are demonstrated using a molecular docking approach. Ultimately, the formation of BAMPs through HMGB1 antagonist glycyrrhizic acid (GA), resulting in impaired immune recognition of myeloid differentiation factor 88 (MYD88) mediated dendritic cells (DCs) and macrophages (Mphis), as well as failed osteoinduction processes is obstructed. This study introduces a mechanism for early immune recognition of implant materials based on protein adsorption, providing perspectives for future design and application of tissue engineering materials. CI - (c) 2023 Wiley-VCH GmbH. FAU - Zhao, Qin AU - Zhao Q AUID- ORCID: 0000-0003-4223-9522 AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Zhao, Zifan AU - Zhao Z AUID- ORCID: 0000-0002-1738-6056 AD - Center of Digital Dentistry, Faculty of Prosthodontics, Peking University School and Hospital of Stomatology; National Center of Stomatology; National Engineeroeing Research Center of Oral Biomaterials and Digital Medical Devices; Beijing Key Laboratory of Digital Stomatology; Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, National Clinical Research Center for Oral Diseases, Beijing, 100081, China. FAU - Zhang, Jing AU - Zhang J AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Ni, Yueqi AU - Ni Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Ouyang, Simin AU - Ouyang S AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Qi, Haoning AU - Qi H AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Yu, Yiqian AU - Yu Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Miron, Richard J AU - Miron RJ AD - Department of Periodontology, University of Bern, Bern, 300392, Switzerland. FAU - Tang, Hua AU - Tang H AD - Department of Rheumatology and Autoimmunology, Shandong Provincial Key Laboratory for Rheumatic Disease and Translational Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250000, China. AD - Institute of Infection and Immunity, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250000, China. FAU - Zhang, Yufeng AU - Zhang Y AUID- ORCID: 0000-0001-8702-5291 AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. LA - eng GR - 82025011/National Natural Science Foundation of China/ GR - 82270981/National Natural Science Foundation of China/ GR - 82100975/National Natural Science Foundation of China/ PT - Journal Article DEP - 20230831 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 RN - 0 (Biocompatible Materials) RN - 0 (Fibronectins) RN - 0 (HMGB1 Protein) RN - 0 (hydroxyapatite-beta tricalcium phosphate) RN - 0 (Hydroxyapatites) SB - IM MH - *Biocompatible Materials/chemistry MH - Fibronectins/chemistry MH - *HMGB1 Protein MH - Adsorption MH - Chromatography, Liquid MH - Molecular Docking Simulation MH - Tandem Mass Spectrometry MH - *Hydroxyapatites OTO - NOTNLM OT - biomaterial-associated molecular patterns OT - fibronectin OT - immune recognition OT - osteoinduction OT - protein adsorptions EDAT- 2023/08/21 12:42 MHDA- 2024/01/16 06:41 CRDT- 2023/08/21 07:29 PHST- 2023/08/11 00:00 [revised] PHST- 2023/06/07 00:00 [received] PHST- 2024/01/16 06:41 [medline] PHST- 2023/08/21 12:42 [pubmed] PHST- 2023/08/21 07:29 [entrez] AID - 10.1002/adhm.202301808 [doi] PST - ppublish SO - Adv Healthc Mater. 2024 Jan;13(2):e2301808. doi: 10.1002/adhm.202301808. Epub 2023 Aug 31.