PMID- 37603176
OWN - NLM
STAT- MEDLINE
DCOM- 20230822
LR  - 20230903
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2700
DP  - 2023
TI  - Endotoxin-Tolerance Mimicking to Study TLR in Promotion of Tolerogenic DCs and 
      Tr1 Cells.
PG  - 93-116
LID - 10.1007/978-1-0716-3366-3_5 [doi]
AB  - Dendritic cells (DCs) are key regulators of immunogenic and tolerogenic immune 
      responses. Both these immune responses require DCs respectively to activate 
      effector T cells or to induce their anergy and T regulatory activity. 
      Modifications of DCs in the laboratory and several pharmacological agents can 
      enhance and stabilize their tolerogenic properties. Recent evidences demonstrate 
      that activation of specific toll-like receptors (TLRs) can be involved in 
      induction of DCs with tolerogenic properties able to initiate T regulatory cell 
      responses.In the present chapter, we show a detail protocol to obtain in vitro 
      regulatory conventional DCs (cDCs) in response to repeated exposure to 
      lipopolysaccharide (LPS), a ligand of TLR4, by mimicking the mechanism of 
      endotoxin tolerance. Subsequently, the protective effect of cDCs' conditionate 
      with LPS will be describe in in vivo inflammatory model of endotoxemia. Finally, 
      we illustrate the method to study the ability of LPS-conditionate cDCs to promote 
      T regulatory cells in ex vivo system.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Scalisi, Giulia
AU  - Scalisi G
AD  - Department of Medicine and Surgery, University of Perugia, Perugia, Italy.
FAU - Ricciuti, Doriana
AU  - Ricciuti D
AD  - Department of Medicine and Surgery, University of Perugia, Perugia, Italy.
FAU - Manni, Giorgia
AU  - Manni G
AD  - Department of Medicine and Surgery, University of Perugia, Perugia, Italy. 
      giorgia.manni@unipg.it.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Lipopolysaccharides)
RN  - Chanarin-Dorfman Syndrome
SB  - IM
MH  - *T-Lymphocytes, Regulatory
MH  - *Endotoxin Tolerance
MH  - Lipopolysaccharides
MH  - Dendritic Cells
OTO - NOTNLM
OT  - Bone marrow dendritic cells
OT  - Endotoxin tolerance
OT  - Immune tolerance
OT  - LPS
OT  - Toll-like receptors
OT  - Treg polarization
EDAT- 2023/08/21 12:43
MHDA- 2023/08/22 06:42
CRDT- 2023/08/21 11:09
PHST- 2023/08/22 06:42 [medline]
PHST- 2023/08/21 12:43 [pubmed]
PHST- 2023/08/21 11:09 [entrez]
AID - 10.1007/978-1-0716-3366-3_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2700:93-116. doi: 10.1007/978-1-0716-3366-3_5.