PMID- 37603218
OWN - NLM
STAT- Publisher
LR  - 20231022
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Print)
IS  - 0914-7470 (Linking)
VI  - 36
IP  - 6
DP  - 2023 Nov
TI  - Comprehensive cell surface protein profiling of human mesenchymal stromal cells 
      from peritoneal dialysis effluent and comparison with those from human bone 
      marrow and adipose tissue.
PG  - 2259-2269
LID - 10.1007/s13577-023-00971-x [doi]
AB  - Peritoneal mesenchymal stromal cells (pMSCs) are isolated from peritoneal 
      dialysis (PD) effluent, and treatment with the pMSCs reduces peritoneal membrane 
      injury in rat model of PD. This study was designed to verify the identity of the 
      pMSCs. pMSCs were grown in plastic dishes for 4-7 passages, and their cell 
      surface phenotype was examined by staining with a panel of 242 antibodies. The 
      positive stain of each target protein was determined by an increase in 
      fluorescence intensity as compared with isotype controls in flow cytometrical 
      analysis. Here, we showed that pMSCs predominantly expressed CD9, CD26, CD29, 
      CD42a, CD44, CD46, CD47, CD49b, CD49c, CD49e, CD54, CD55, CD57, CD59, CD63, CD71, 
      CD73, CD81, CD90, CD98, CD147, CD151, CD200, CD201, beta2-micoglobulin, epithelial 
      growth factor receptor, human leukocyte antigen (HLA) class 1, and, to a lesser 
      extent, CD31, CD45RO, CD49a, CD49f, CD50, CD58, CD61, CD105, CD164, and CD166. 
      These cells lacked expression of most hematopoietic markers such as CD11b, CD14, 
      CD19, CD34, CD40, CD80, CD79, CD86, and HLA-DR. There was 38.55% difference in 
      the expression of 83 surface proteins between bone marrow (BM)-derived MSCs and 
      pMSCs, and 14.1% in the expression of 242 proteins between adipose tissue 
      (AT)-derived MSCs and pMSCs. The BM-MSCs but not both AT-MSCs and pMSCs express 
      cytokine receptors (IFNgammaR, TNFI/IIR, IL-1R, IL-4R, IL-6R, and IL-7R). In 
      conclusion, pMSCs exhibited a typical cell surface phenotype of MSCs, which was 
      not the same as on BM-MSCs or AT-MSCs, suggesting that the pMSCs may represent a 
      different MSC lineage from peritoneal cavity.
CI  - (c) 2023. The Author(s).
FAU - Shi, Ganggang
AU  - Shi G
AD  - Department of Colorectal Surgery, The Second Hospital of Tianjin Medical 
      University, Tianjin, People's Republic of China.
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada.
FAU - Yang, Chong
AU  - Yang C
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada.
AD  - Organ Transplantation Center, School of Medicine, Sichuan Academy of Medical 
      Sciences, Sichuan Provincial People's Hospital, University of Electronic Science 
      and Technology of China, Chengdu, Sichuan, People's Republic of China.
FAU - Zhou, Lan
AU  - Zhou L
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada.
AD  - Department of Urology, Shanghai United Family Hospital, Shanghai, People's 
      Republic of China.
FAU - Zong, Ming
AU  - Zong M
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada.
AD  - Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People's 
      Republic of China.
FAU - Guan, Qiunong
AU  - Guan Q
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada.
FAU - da Roza, Gerald
AU  - da Roza G
AD  - Division of Nephrology, Department of Medicine, The University of British 
      Columbia, Vancouver, BC, Canada.
FAU - Wang, Hao
AU  - Wang H
AD  - Department of General Surgery, Tianjin Medical University General Hospital, 
      Tianjin, People's Republic of China.
FAU - Qi, Hualin
AU  - Qi H
AD  - Department of Nephrology, Shanghai Pudong New Area People's Hospital, 490 
      Chuanhuan Nan Lu, Pudong New Area, Shanghai, 201299, People's Republic of China. 
      qihualin@126.com.
FAU - Du, Caigan
AU  - Du C
AUID- ORCID: 0000-0001-8605-5501
AD  - Department of Urologic Sciences, The University of British Columbia, Vancouver, 
      BC, Canada. caigan@mail.ubc.ca.
AD  - Jack Bell Research Centre, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada. 
      caigan@mail.ubc.ca.
LA  - eng
PT  - Journal Article
DEP - 20230821
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
SB  - IM
PMC - PMC10587256
OTO - NOTNLM
OT  - Adipose MSCs
OT  - Bone marrow MSCs
OT  - Cell surface protein phenotype
OT  - MSC lineage
OT  - Peritoneal mesenchymal stromal cells
COIS- The authors declare that there are no conflict of interest.
EDAT- 2023/08/21 12:42
MHDA- 2023/08/21 12:42
PMCR- 2023/08/21
CRDT- 2023/08/21 11:11
PHST- 2023/06/20 00:00 [received]
PHST- 2023/08/07 00:00 [accepted]
PHST- 2023/08/21 12:42 [pubmed]
PHST- 2023/08/21 12:42 [medline]
PHST- 2023/08/21 11:11 [entrez]
PHST- 2023/08/21 00:00 [pmc-release]
AID - 10.1007/s13577-023-00971-x [pii]
AID - 971 [pii]
AID - 10.1007/s13577-023-00971-x [doi]
PST - ppublish
SO  - Hum Cell. 2023 Nov;36(6):2259-2269. doi: 10.1007/s13577-023-00971-x. Epub 2023 
      Aug 21.