PMID- 3760564
OWN - NLM
STAT- MEDLINE
DCOM- 19861107
LR  - 20041117
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 137
IP  - 8
DP  - 1986 Oct 15
TI  - Implantation of cultured thymic fragments in congenitally athymic nude rats: 
      ignorance of thymic epithelial haplotype in generation of alloreactivity.
PG  - 2440-7
AB  - We studied the development of thymus-dependent immunity in congenitally athymic 
      nude rats after implantation of cultured thymic fragments (CTF), particularly the 
      development of in vitro alloreactivity in allogeneic combinations. CTF of DA 
      (RT1a), PVG (RT1c), and RP (RT1p(u,1] origin were implanted in nude rats of WAG 
      (RT1u) origin. In analysis 14 to 18 wk later, all recipients exhibited 
      thymus-dependent immunocompetence assessed by (immuno)-histology of lymphoid 
      organs and responsiveness to in vitro concanavalin A stimulation and in vivo 
      ovalbumin immunization. Control nude animals were unresponsive. Also, in vitro 
      alloreactivity was observed, measured by mixed lymphocyte reaction and 
      cell-mediated lympholysis. The alloresponse to the allogeneic CTF donor haplotype 
      was as to a third party, but that to the recipient was negative. The CTF before 
      implantation were devoid of lymphoid elements and revealed epithelial-like cells 
      as the major component. Cells in CTF showed expression of RT1 class I and class 
      II antigens. CTF at autopsy had the architecture of a normal thymus. In 
      immunohistochemistry using haplotype-specific antibodies, lymphocytes showed RT1u 
      class I expression as in the normal WAG thymus. In the cortex-like area of CTF, 
      stromal cells revealed class I and class II haplotype expression of the donor 
      thymus, but in the medulla-like area, class II haplotype expression was that of 
      the recipient WAG rat. These data indicate that after implantation in nude rats, 
      CTF become populated not only with lymphoid elements, but also with stromal 
      components from the recipient. In induction of thymus-dependent immunity, these 
      acceptor-derived stromal (dendritic) cells may be involved in generation of 
      allospecificity; class I and class II haplotype expression by the donor cortex 
      (epithelial) compartment is ignored in this process.
FAU - Schuurman, H J
AU  - Schuurman HJ
FAU - Vaessen, L M
AU  - Vaessen LM
FAU - Vos, J G
AU  - Vos JG
FAU - Hertogh, A
AU  - Hertogh A
FAU - Geertzema, J G
AU  - Geertzema JG
FAU - Brandt, C J
AU  - Brandt CJ
FAU - Rozing, J
AU  - Rozing J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Epithelium/immunology
MH  - Haplotypes
MH  - Male
MH  - Organ Culture Techniques
MH  - Rats
MH  - Rats, Mutant Strains/*immunology
MH  - Rats, Nude/*immunology
MH  - Thymus Gland/cytology/immunology/*transplantation
MH  - Transplantation, Homologous
EDAT- 1986/10/15 00:00
MHDA- 1986/10/15 00:01
CRDT- 1986/10/15 00:00
PHST- 1986/10/15 00:00 [pubmed]
PHST- 1986/10/15 00:01 [medline]
PHST- 1986/10/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1986 Oct 15;137(8):2440-7.