PMID- 37606789 OWN - NLM STAT- MEDLINE DCOM- 20231009 LR - 20231018 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 107 IP - 20 DP - 2023 Oct TI - Exploring a high-urease activity Bacillus cereus for self-healing concrete via induced CaCO(3) precipitation. PG - 6351-6362 LID - 10.1007/s00253-023-12725-8 [doi] AB - The structural integrity and esthetic appeal of concrete can be compromised by concrete cracks. Promise has been shown by microbe-induced calcium carbonate precipitation (MICP) as a solution for concrete cracking, with a focus on urease-producing microorganisms in research. Bacillus cereus was isolated from soil and employed for this purpose in this study due to its high urease activity. The strain exhibited strong tolerance for alkaline media and high salt levels, which grew at a pH of 13 and 4% salt concentration. The repair of concrete cracks with this strain was evaluated by assessing the effects of four different thickeners at varying concentrations. The most effective results were achieved with 10 g/L of sodium carboxymethyl cellulose (CMC-Na). The data showed that over 90% repair of cracks was achieved by this system with an initial water penetration time of 30 s. The study also assessed the quantity and sizes of crystals generated during the bacterial mineralization process over time to improve our understanding of the process. KEY POINTS: * MICP using Bacillus cereus shows potential for repairing concrete cracks. * Strain tolerates alkaline media and high salt levels, growing at pH 13 and 4% salt concentration. * Sodium carboxymethyl cellulose (CMC-Na) at 10 g/L achieved over 90% repair of cracks. CI - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Xu, Jian-Miao AU - Xu JM AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Wang, Wei-Jie AU - Wang WJ AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Chen, Zhuo-Ting AU - Chen ZT AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Zhou, Yuan-Yuan AU - Zhou YY AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Pan, Jia-Jia AU - Pan JJ AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Cheng, Feng AU - Cheng F AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. FAU - Liu, Zhi-Qiang AU - Liu ZQ AUID- ORCID: 0000-0003-3259-6796 AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. microliu@zjut.edu.cn. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. microliu@zjut.edu.cn. FAU - Zheng, Yu-Guo AU - Zheng YG AD - Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, The National and Local, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. AD - Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China. LA - eng PT - Journal Article DEP - 20230822 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - EC 3.5.1.5 (Urease) RN - K679OBS311 (Carboxymethylcellulose Sodium) RN - H0G9379FGK (Calcium Carbonate) RN - 451W47IQ8X (Sodium Chloride) RN - 9NEZ333N27 (Sodium) SB - IM MH - *Bacillus cereus MH - *Bacillus MH - Urease MH - Carboxymethylcellulose Sodium MH - Calcium Carbonate/chemistry MH - Sodium Chloride MH - Sodium MH - Chemical Precipitation MH - Construction Materials/microbiology OTO - NOTNLM OT - Bacillus cereus OT - Particle Track G400 OT - Self-healing concrete OT - Thickener EDAT- 2023/08/22 13:42 MHDA- 2023/10/09 06:42 CRDT- 2023/08/22 11:19 PHST- 2023/05/12 00:00 [received] PHST- 2023/08/06 00:00 [accepted] PHST- 2023/07/29 00:00 [revised] PHST- 2023/10/09 06:42 [medline] PHST- 2023/08/22 13:42 [pubmed] PHST- 2023/08/22 11:19 [entrez] AID - 10.1007/s00253-023-12725-8 [pii] AID - 10.1007/s00253-023-12725-8 [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2023 Oct;107(20):6351-6362. doi: 10.1007/s00253-023-12725-8. Epub 2023 Aug 22.