PMID- 37607465
OWN - NLM
STAT- Publisher
LR  - 20231017
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 124
IP  - Pt A
DP  - 2023 Nov
TI  - Chitinase 3-like 1 plays a pivotal role in airway response of RSV infection via 
      regulating DC functional transition.
PG  - 110819
LID - S1567-5769(23)01144-X [pii]
LID - 10.1016/j.intimp.2023.110819 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) contribute to immune imbalance and airway 
      hyperresponsiveness (AHR) induced by respiratory syncytial virus (RSV). The aim 
      of present study was to explore the mechanism of RSV regulating naive T cell 
      differentiation through DCs. METHODS: We generated a Lentivirus shRNA expression 
      vector to knock down CHI3L1 in mouse lungs and bone marrow-derived dendritic 
      cells (BMDCs). Then we investigated the effect of CHI3L1 knockdown on MAPK/ERK 
      pathway, PI3K/AKT pathway, mature DCs represented by molecular markers, naive T 
      cell differentiation and related cytokine expression in vitro and in vivo models 
      of RSV. RESULTS: RSV elevated CHI3L1 expression in lung DCs and BMDCs. Knockdown 
      of CHI3L1 impeded RSV-induced activation of MAPK/ERK and PI3K/AKT signaling 
      pathways, attenuated CD86 and OX40L expression in mature DCs, reduced the 
      proportion of Th2 and Th17 cells, and increased the proportion of Treg cells. In 
      addition, by blocking CHI3L1, RSV-infected mice shown relief of airway 
      resistance, the downregulation of Th2/Th17 like cytokines IL-4, IL-13 and IL-17 
      levels, and the upregulation of IL-10. CONCLUSION: Our data show that CHI3L1 
      promotes RSV induced immune imbalance and airway hyperresponsiveness by 
      regulating the functional transformation of DCs.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Ge, Lingli
AU  - Ge L
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 
      China; Department of Pediatrics, Xiangyang Central Hospital, Affiliated Hospital 
      of Hubei University of Arts and Science, Xiangyang, Hubei, China. Electronic 
      address: gelingli0505@163.com.
FAU - Wang, Yuxin
AU  - Wang Y
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 
      China. Electronic address: 751026394@qq.com.
FAU - Liu, Zhi
AU  - Liu Z
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 
      China. Electronic address: 83972576@qq.com.
FAU - Du, Hui
AU  - Du H
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 
      China. Electronic address: 13407180763@163.com.
FAU - Zhao, Dongchi
AU  - Zhao D
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 
      China; Children's digital health and data Center of Wuhan University, Wuhan, 
      Hubei, China. Electronic address: zhao_wh2004@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20230820
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
SB  - IM
OTO - NOTNLM
OT  - Chitinase 3-like 1
OT  - Immune response
OT  - Immunogenic maturation
OT  - Lung dendritic cells
OT  - Respiratory syncytial virus
OT  - T lymphocytes
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/23 00:41
MHDA- 2023/08/23 00:41
CRDT- 2023/08/22 18:06
PHST- 2023/06/11 00:00 [received]
PHST- 2023/08/11 00:00 [revised]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/08/23 00:41 [pubmed]
PHST- 2023/08/23 00:41 [medline]
PHST- 2023/08/22 18:06 [entrez]
AID - S1567-5769(23)01144-X [pii]
AID - 10.1016/j.intimp.2023.110819 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2023 Nov;124(Pt A):110819. doi: 
      10.1016/j.intimp.2023.110819. Epub 2023 Aug 20.