PMID- 37611433
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231026
IS  - 1879-0593 (Electronic)
IS  - 1368-8375 (Linking)
VI  - 146
DP  - 2023 Nov
TI  - Mycobiota profile of oral fungal infections in head and neck cancer patients 
      receiving radiotherapy: A 6-year retrospective MALDI-TOF mass spectrometry study.
PG  - 106556
LID - S1368-8375(23)00252-X [pii]
LID - 10.1016/j.oraloncology.2023.106556 [doi]
AB  - OBJECTIVES: Head and neck cancer (HNC) impairs patient immunity and increases 
      susceptibility to oral fungal infections (OFIs). Effectively treating such 
      infections requires accurate identification of the causative pathogens. This 
      study aimed to characterize the mycobiota profile of OFIs in HNC patients 
      undergoing radiation treatment (RT). MATERIALS AND METHODS: A 6-year 
      retrospective analysis of oral mucosal samples from HNC patients with a history 
      of RT and OFIs between 2014 and 2019 was conducted using Matrix-Assisted Laser 
      Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling. 
      Samples from the Clinical Microbiology Laboratory at Karolinska University 
      Hospital were evaluated for mycobiota diversity and species co-occurrence 
      patterns in the ongoing-RT and post-RT groups. RESULTS: A total of 190 oral fungi 
      (88% Candida, 5% Pichia) were isolated from 162 HNC patients receiving RT. In the 
      ongoing-RT group, the emergent non-albicans Candida (NAC) species; F. solani and 
      C. jadinii, were detected for the first time. The dominant pathogens in both 
      ongoing and post-RT groups were C. albicans, C. glabrata, P. kudriavzevii, C. 
      parapsilosis, and C. tropicalis, as shown by Venn analysis. Network analysis 
      revealed greater fungi diversity and multi-species co-occurrence in the 
      ongoing-RT group. C. albicans commonly co-occurred with C. glabrata in both 
      ongoing-RT (21%) and post-RT groups (30%). CONCLUSION: MALDI-TOF MS identified a 
      wide range of oral fungal species in HNC patients receiving RT. While C. albicans 
      remains the most prevalent OFIs pathogen, multi-species co-occurrence and novel 
      NACs were noted. Understanding the ecological interactions among these causative 
      pathogens could significantly advance the development of effective therapeutics 
      for treating OFIs in HNC patients.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Al-Manei, Khaled
AU  - Al-Manei K
AD  - Unit of Oral Diagnostics and Surgery, Department of Dental Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden; Division of Endodontics, Department of 
      Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 
      11545, Saudi Arabia.
FAU - Sobkowiak, Michal Jacek
AU  - Sobkowiak MJ
AD  - Unit of Oral Diagnostics and Surgery, Department of Dental Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden.
FAU - Nagadia, Rahul Harshad
AU  - Nagadia RH
AD  - Unit of Oral Diagnostics and Surgery, Department of Dental Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden; Department of Head and Neck Surgery, National 
      Cancer Centre Singapore, and Singapore General Hospital, Singapore; Department of 
      Oral and Maxillofacial Surgery, National Dental Centre Singapore, Singapore.
FAU - Heymann, Robert
AU  - Heymann R
AD  - Unit of Oral Diagnostics and Surgery, Department of Dental Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden; Medical Unit for Reconstructive Plastic- and 
      Craniofacial Surgery, Karolinska University Hospital, Stockholm, Sweden.
FAU - Sallberg Chen, Margaret
AU  - Sallberg Chen M
AD  - Unit of Oral Diagnostics and Surgery, Department of Dental Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden. Electronic address: Margaret.Chen@ki.se.
FAU - Ozenci, Volkan
AU  - Ozenci V
AD  - Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska 
      Institutet, Huddinge 14104, Sweden; Department of Clinical Microbiology F72, 
      Karolinska University Hospital, Huddinge 14104, Sweden. Electronic address: 
      Volkan.Ozenci@ki.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230821
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
RN  - Pichia kudriavzevii
SB  - IM
MH  - Humans
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Retrospective Studies
MH  - Candida/chemistry
MH  - *Mycoses
MH  - *Head and Neck Neoplasms/radiotherapy
OTO - NOTNLM
OT  - Cancer
OT  - Candida albicans
OT  - Fungal infection
OT  - Human
OT  - Matrix-assisted laser desorption-ionization
OT  - Mycobiota
OT  - Radiation
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/24 00:42
MHDA- 2023/10/23 00:43
CRDT- 2023/08/23 18:03
PHST- 2023/04/04 00:00 [received]
PHST- 2023/07/14 00:00 [revised]
PHST- 2023/08/13 00:00 [accepted]
PHST- 2023/10/23 00:43 [medline]
PHST- 2023/08/24 00:42 [pubmed]
PHST- 2023/08/23 18:03 [entrez]
AID - S1368-8375(23)00252-X [pii]
AID - 10.1016/j.oraloncology.2023.106556 [doi]
PST - ppublish
SO  - Oral Oncol. 2023 Nov;146:106556. doi: 10.1016/j.oraloncology.2023.106556. Epub 
      2023 Aug 21.