PMID- 37611690
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231122
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 252
DP  - 2023 Dec 1
TI  - Actinidia eriantha polysaccharide exerts adjuvant activity by targeting linc-AAM.
PG  - 126440
LID - S0141-8130(23)03336-6 [pii]
LID - 10.1016/j.ijbiomac.2023.126440 [doi]
AB  - Actinidia eriantha polysaccharide (AEPS) is a potent adjuvant with dual Th1 and 
      Th2 potentiating activity. linc-AAM has been previously proved to facilitate the 
      expression of immune response genes (IRGs) in AEPS-activated RAW264.7 
      macrophages. However, its role in mediating adjuvant activity of AEPS remains to 
      be elucidated. In this study, bone marrow-derived macrophages (BMDMs) from 
      wide-type (WT) and linc-AAM knockout C57BL/6J mice treated with AEPS were 
      subjected to transcriptome sequencing and bioinformatic analysis. linc-AAM 
      deficiency inhibited M1 and M2 immune responses in BMDMs induced by AEPS. In 
      mechanisms, AEPS facilitated the expression of IRGs and activated BMDMs through 
      NF-kappaB-linc-AAM-JAK/STAT axis. Furthermore, linc-AAM knockout inhibited cytokine 
      and chemokine production, immune cell recruitment as well as immune cell 
      migration to draining lymph nodes at peritoneal cavity in mice induced by AEPS. 
      More importantly, linc-AAM deletion reduced the adjuvant activity of APES on 
      antigen-specific cellular and humoral immune responses to ovalbumin in mice. This 
      study has for the first time demonstrated the role of lncRNAs in regulating the 
      adjuvant activity of polysaccharides and its mechanisms. These findings expanded 
      current knowledge on the mechanism of action of adjuvant and provide a new target 
      for the design and development of vaccine adjuvants.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Xiong, Beibei
AU  - Xiong B
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China.
FAU - Chen, Xiangfeng
AU  - Chen X
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China.
FAU - Tu, Jue
AU  - Tu J
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China; Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, 
      Hangzhou 310058, Zhejiang, China.
FAU - Han, Ziyi
AU  - Han Z
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China.
FAU - Meng, Xiang
AU  - Meng X
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China.
FAU - Sun, Hongxiang
AU  - Sun H
AD  - College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, 
      China. Electronic address: sunhx@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230821
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Adjuvants, Pharmaceutic)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Actinidia/genetics
MH  - Mice, Inbred C57BL
MH  - Adjuvants, Immunologic/pharmacology/metabolism
MH  - Macrophages
MH  - Adjuvants, Pharmaceutic
MH  - Polysaccharides/pharmacology/metabolism
OTO - NOTNLM
OT  - Actinidia eriantha polysaccharide
OT  - Adjuvant
OT  - Large intergenic noncoding RNA (lincRNA)
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/24 00:42
MHDA- 2023/11/15 06:43
CRDT- 2023/08/23 19:23
PHST- 2023/04/25 00:00 [received]
PHST- 2023/07/17 00:00 [revised]
PHST- 2023/08/18 00:00 [accepted]
PHST- 2023/11/15 06:43 [medline]
PHST- 2023/08/24 00:42 [pubmed]
PHST- 2023/08/23 19:23 [entrez]
AID - S0141-8130(23)03336-6 [pii]
AID - 10.1016/j.ijbiomac.2023.126440 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Dec 1;252:126440. doi: 10.1016/j.ijbiomac.2023.126440. 
      Epub 2023 Aug 21.