PMID- 3761198
OWN - NLM
STAT- MEDLINE
DCOM- 19861117
LR  - 20071114
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 239
IP  - 1
DP  - 1986 Oct
TI  - Monoiodinated angiotensin II is a potent, full agonist analog of angiotensin II.
PG  - 71-7
AB  - Mono 125I-angiotensin II (Ang II) has been used extensively as a radioligand to 
      identify Ang II receptors whereas its receptor binding properties are well 
      characterized, its biological activity has been less well studied. To examine 
      this issue, nonradioisotopic monoiodo-Ang II was prepared and compared to Ang II. 
      Monoiodo-Ang II was found to be a potent, full agonist in in vivo bioassays and a 
      more potent (2.5-fold) pressor agent than the native hormone Ang II in the pithed 
      rat. In eliciting dipsogenic responses monoiodo-Ang II was equipotent to Ang II, 
      but was less potent (2.7-fold) than Ang II in contracting rat aortic strips. 
      These results suggest that the well characterized binding affinity of 
      monoiodo-Ang II is representative of its biological activity (40-250% of the 
      activity of Ang II). The variation in relative peptide potency is consistent with 
      the hypothesis of a heterogeneity of Ang II receptors. Most importantly, the 
      similar efficacies between Ang II and monoiodo-Ang II indicate that the 
      monoiodinated Ang II is a suitable ligand for the study of Ang II receptors.
FAU - Husain, A
AU  - Husain A
FAU - Pajka, S F
AU  - Pajka SF
FAU - Taylor, S M
AU  - Taylor SM
FAU - Speth, R C
AU  - Speth RC
LA  - eng
GR  - HL-27568/HL/NHLBI NIH HHS/United States
GR  - HL-6835/HL/NHLBI NIH HHS/United States
GR  - SO7 RR05465/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Receptors, Angiotensin)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/*analogs & derivatives
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Thin Layer
MH  - Iodine Radioisotopes
MH  - Isotope Labeling
MH  - Mathematics
MH  - Rats
MH  - Receptors, Angiotensin/metabolism
EDAT- 1986/10/01 00:00
MHDA- 1986/10/01 00:01
CRDT- 1986/10/01 00:00
PHST- 1986/10/01 00:00 [pubmed]
PHST- 1986/10/01 00:01 [medline]
PHST- 1986/10/01 00:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 1986 Oct;239(1):71-7.