PMID- 37614212
OWN - NLM
STAT- MEDLINE
DCOM- 20231120
LR  - 20240508
IS  - 2042-7670 (Electronic)
IS  - 0042-4900 (Linking)
VI  - 193
IP  - 10
DP  - 2023 Nov 18
TI  - Cross-reactive carbohydrate determinants in atopic and healthy dogs and their 
      influence on allergy test specificity.
PG  - e3308
LID - 10.1002/vetr.3308 [doi]
AB  - BACKGROUND: The selection of allergens for immunotherapy in atopic dogs is often 
      based on serum allergy testing. Cross-reactive carbohydrate determinants (CCDs) 
      are common structures in plant and insect allergens that reportedly induce 
      polysensitisation, reduce agreement between intradermal and serum tests and 
      complicate allergen selection. METHODS: Thirty-four dogs with diagnosed atopic 
      dermatitis and 10 healthy dogs were included in the study. An intradermal test 
      was conducted in atopic dogs, and serum samples from allergic and healthy dogs 
      were analysed for allergen-specific immunoglobulin E (IgE) before and after 
      inhibition of detectable anti-CCD-IgE antibodies. RESULTS: Anti-CCD-IgE 
      antibodies were not found in any of the healthy dogs and no polysensitisation to 
      plant and insect allergens was detected. The agreement between intradermal and 
      serum allergy test results in the atopic dogs with anti-CCD-IgE antibodies 
      improved from slight to fair after blocking the anti-CCD-IgE antibodies. In 
      addition, blocking clearly reduced polysensitisation to plant allergens but not 
      to acarid allergens. LIMITATIONS: Only a limited number of healthy dogs were 
      tested in this study. A gold standard for determining the clinical relevance of 
      IgE sensitisation does not exist. CONCLUSION: Inhibition of anti-CCD-IgE 
      antibodies seems to be of importance to improve serum test specificity for 
      allergen-specific IgE in atopic dogs in relation to intradermal allergy testing.
CI  - (c) 2023 The Authors. Veterinary Record published by John Wiley & Sons Ltd on 
      behalf of British Veterinary Association.
FAU - Kasper, Bettina
AU  - Kasper B
AUID- ORCID: 0000-0002-2876-5529
AD  - Center of Clinical Veterinary Medicine, LMU Munich, Munich, Germany.
FAU - Boehm, Teresa
AU  - Boehm T
AD  - Center of Clinical Veterinary Medicine, LMU Munich, Munich, Germany.
FAU - Wittenstein, Nadine
AU  - Wittenstein N
AD  - Tierklinik Germering, Germering, Germany.
FAU - Mueller, Ralf S
AU  - Mueller RS
AUID- ORCID: 0000-0001-5835-5910
AD  - Center of Clinical Veterinary Medicine, LMU Munich, Munich, Germany.
LA  - eng
PT  - Journal Article
DEP - 20230824
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (Allergens)
RN  - 0 (Carbohydrates)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Dogs
MH  - Animals
MH  - Allergens
MH  - *Hypersensitivity/diagnosis/veterinary
MH  - *Dermatitis, Atopic/diagnosis/veterinary
MH  - Carbohydrates
MH  - Immunoglobulin E
MH  - *Dog Diseases/diagnosis
EDAT- 2023/08/24 06:42
MHDA- 2023/11/20 06:55
CRDT- 2023/08/24 03:53
PHST- 2023/07/01 00:00 [revised]
PHST- 2023/03/09 00:00 [received]
PHST- 2023/07/07 00:00 [accepted]
PHST- 2023/11/20 06:55 [medline]
PHST- 2023/08/24 06:42 [pubmed]
PHST- 2023/08/24 03:53 [entrez]
AID - 10.1002/vetr.3308 [doi]
PST - ppublish
SO  - Vet Rec. 2023 Nov 18;193(10):e3308. doi: 10.1002/vetr.3308. Epub 2023 Aug 24.