PMID- 37616194
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230904
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 8
DP  - 2023
TI  - All-synchronized picosecond pulses and time-gated detection improve the spatial 
      resolution of two-photon STED microscopy in brain tissue imaging.
PG  - e0290550
LID - 10.1371/journal.pone.0290550 [doi]
LID - e0290550
AB  - Super-resolution in two-photon excitation (2PE) microscopy offers new approaches 
      for visualizing the deep inside the brain functions at the nanoscale. In this 
      study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope 
      with all-synchronized picosecond pulse light sources and time-gated fluorescence 
      detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of 
      time-gating is critical to excluding undesirable signals derived from brain 
      tissues. Even in a case using subnanosecond pulses for STED, the impact of 
      time-gating was not negligible; the spatial resolution in the image of the brain 
      tissue was improved by approximately 1.4 times compared with non time-gated 
      image. This finding demonstrates that time-gating is more useful than previously 
      thought for improving spatial resolution in brain tissue imaging. This microscopy 
      will facilitate deeper super-resolution observation of the fine structure of 
      neuronal dendritic spines and the intracellular dynamics in brain tissue.
CI  - Copyright: (c) 2023 Ishii et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Ishii, Hirokazu
AU  - Ishii H
AUID- ORCID: 0000-0001-8391-194X
AD  - Exploratory Research Center on Life and Living Systems (ExCELLS), National 
      Institutes of Natural Sciences, Okazaki, Japan.
AD  - National Institute for Physiological Sciences (NIPS), National Institutes of 
      Natural Sciences, Okazaki, Japan.
AD  - School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 
      Okazaki, Japan.
FAU - Otomo, Kohei
AU  - Otomo K
AD  - Exploratory Research Center on Life and Living Systems (ExCELLS), National 
      Institutes of Natural Sciences, Okazaki, Japan.
AD  - National Institute for Physiological Sciences (NIPS), National Institutes of 
      Natural Sciences, Okazaki, Japan.
AD  - Graduate School of Medicine, Juntendo University, Tokyo, Japan.
FAU - Chang, Ching-Pu
AU  - Chang CP
AD  - Exploratory Research Center on Life and Living Systems (ExCELLS), National 
      Institutes of Natural Sciences, Okazaki, Japan.
AD  - National Institute for Physiological Sciences (NIPS), National Institutes of 
      Natural Sciences, Okazaki, Japan.
AD  - Faculty of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Yamasaki, Miwako
AU  - Yamasaki M
AD  - Faculty of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Watanabe, Masahiko
AU  - Watanabe M
AD  - Faculty of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Yokoyama, Hiroyuki
AU  - Yokoyama H
AD  - New Industry Creation Hatchery Center (NICHe), Tohoku University, Sendai, Japan.
FAU - Nemoto, Tomomi
AU  - Nemoto T
AUID- ORCID: 0000-0001-6102-1495
AD  - Exploratory Research Center on Life and Living Systems (ExCELLS), National 
      Institutes of Natural Sciences, Okazaki, Japan.
AD  - National Institute for Physiological Sciences (NIPS), National Institutes of 
      Natural Sciences, Okazaki, Japan.
AD  - School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 
      Okazaki, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230824
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - *Microscopy
MH  - Brain/diagnostic imaging
MH  - Heart Rate
MH  - *Household Articles
MH  - Photons
PMC - PMC10449175
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/08/24 18:41
MHDA- 2023/08/28 06:42
PMCR- 2023/08/24
CRDT- 2023/08/24 13:32
PHST- 2023/04/19 00:00 [received]
PHST- 2023/08/09 00:00 [accepted]
PHST- 2023/08/28 06:42 [medline]
PHST- 2023/08/24 18:41 [pubmed]
PHST- 2023/08/24 13:32 [entrez]
PHST- 2023/08/24 00:00 [pmc-release]
AID - PONE-D-23-09651 [pii]
AID - 10.1371/journal.pone.0290550 [doi]
PST - epublish
SO  - PLoS One. 2023 Aug 24;18(8):e0290550. doi: 10.1371/journal.pone.0290550. 
      eCollection 2023.