PMID- 37619316
OWN - NLM
STAT- MEDLINE
DCOM- 20230918
LR  - 20230921
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 678
DP  - 2023 Oct 20
TI  - Matrix stiffness-mediated tenogenesis of tendon stem/progenitor cells via 
      integrin-alpham for tendon regeneration.
PG  - 90-96
LID - S0006-291X(23)00937-3 [pii]
LID - 10.1016/j.bbrc.2023.08.007 [doi]
AB  - Tendon injuries, commonly associated with sports activities, pose significant 
      challenges in terms of treatment and recovery due to limited tendon regeneration 
      and the formation of proliferative scars. Stem cell-based therapy has shown 
      promising application, but there are still challenges. Physical and biological 
      cues are instrumental in guiding stem cell differentiation and maturation. This 
      study focuses on exploring the effects of matrix biomechanics on tendon 
      stem/progenitor cells (TSPCs) differentiation. We fabricated polydimethylsiloxane 
      (PDMS) substrates with different elastic modulus to mimic the mechanical 
      characteristics of healthy tendons. A tissue-engineered culture system was 
      developed for tenogenesis, and pre-differentiated tissue-engineered tendons were 
      transplanted in vivo to assess their efficacy in regenerating patella tendon 
      injuries. Furthermore, we demonstrated that the biomechanical stimuli activated 
      the integrin-alpham to enhance the tenogenesis capacity of TSPCs. Our findings 
      highlight the importance of biomechanics in tendon tissue engineering and provide 
      a novel perspective for enhancing tendon regeneration.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Zhang, Yuxiang
AU  - Zhang Y
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Department 
      of Plastic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of 
      Medicine, 3 East Qingchun Road, Hangzhou, 310016, Zhejiang Province, PR China.
FAU - Zhang, Erchen
AU  - Zhang E
AD  - The Institute of Reproduction and Stem Cell Engineering, Central South 
      University, Hunan Province, PR China.
FAU - Qin, Tian
AU  - Qin T
AD  - Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang 
      Province, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR 
      China.
FAU - Liu, Mengyun
AU  - Liu M
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Key 
      Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China.
FAU - Zhou, Sicheng
AU  - Zhou S
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Key 
      Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China.
FAU - Lin, Ruifu
AU  - Lin R
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Key 
      Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China.
FAU - Shen, Weiliang
AU  - Shen W
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Key 
      Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China. 
      Electronic address: wlshen@zju.edu.cn.
FAU - Chen, Xiao
AU  - Chen X
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang 
      University School of Medicine, Hangzhou, Zhejiang Province, PR China; Key 
      Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China. 
      Electronic address: chenxiao-610@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230817
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CD11b Antigen)
SB  - IM
MH  - Humans
MH  - CD11b Antigen
MH  - *Tendons
MH  - *Tendon Injuries/therapy
MH  - Biomechanical Phenomena
MH  - Stem Cells
OTO - NOTNLM
OT  - Stiffness
OT  - Tendon regeneration
OT  - Tendon stem/progenitor cells (TSPCs)
OT  - Tissue engineering
COIS- Declaration of competing interest The authors declare that there are no conflicts 
      of interests that may have impacted the research presented in this manuscript.
EDAT- 2023/08/25 00:42
MHDA- 2023/09/18 12:44
CRDT- 2023/08/24 18:01
PHST- 2023/07/14 00:00 [received]
PHST- 2023/07/25 00:00 [revised]
PHST- 2023/08/02 00:00 [accepted]
PHST- 2023/09/18 12:44 [medline]
PHST- 2023/08/25 00:42 [pubmed]
PHST- 2023/08/24 18:01 [entrez]
AID - S0006-291X(23)00937-3 [pii]
AID - 10.1016/j.bbrc.2023.08.007 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2023 Oct 20;678:90-96. doi: 
      10.1016/j.bbrc.2023.08.007. Epub 2023 Aug 17.