PMID- 37622701
OWN - NLM
STAT- Publisher
LR  - 20230825
IS  - 1873-4316 (Electronic)
IS  - 1389-2010 (Linking)
DP  - 2023 Aug 24
TI  - 5-Methylthiopentyl Isothiocyanate, a Sulforaphane Analogue, Inhibits 
      Pro-inflammatory Cytokines by Regulating LPS/ATP-mediated NLRP3 Inflammasome 
      Activation.
LID - 10.2174/1389201024666230824093927 [doi]
AB  - BACKGROUND: Pro-inflammatory cytokines secreted from activated macrophages and 
      astrocytes are crucial mediators of inflammation for host defense. Among them, 
      the secretion of IL-1beta, a major pro-inflammatory cytokine, is especially mediated 
      by the activation of NLRP3 inflammasome. Pro-IL-1beta, which is produced in response 
      to the invaded pathogens, such as LPS, is cleaved and matured in the NLRP3 
      inflammasome by the recognition of ATP. Excessively activated IL-1beta induces other 
      immune cells, resulting in the up-regulation of inflammation. Therefore, 
      regulation of NLRP3 inflammasome can be a good strategy for alleviating 
      inflammation. OBJECTIVE: Our study aimed to examine whether 5-methylthiopentyl 
      isothiocyanate, a sulforaphane analogue (berteroin), has an anti-inflammatory 
      effect on the NLRP3 inflammasome activation induced by LPS and ATP. METHODS: 
      Primary bone marrow-derived macrophages (BMDMs) and astrocytes were stimulated by 
      LPS and ATP with the treatment of 5-methylthiopentyl isothiocyanate, a 
      sulforaphane analogue. The secretion of pro-inflammatory cytokines was measured 
      by ELISA, and the expression level of NLRP3 inflammasome-associated proteins was 
      detected by western blot. The association of NLRP3 inflammasome was assessed by 
      co-immunoprecipitation, and the formation of ASC specks was evaluated by 
      fluorescent microscope. RESULTS: 5-Methylthiopentyl isothiocyanate, a 
      sulforaphane analogue (berteroin), decreased the release of pro-inflammatory 
      cytokines, IL-1beta, and IL-6 in the BMDMs. Berteroin notably prevented the 
      formation of both NLRP3 inflammasome and ASC specks, which reduced the secretion 
      of IL-1beta. Additionally, berteroin reduced the IL-1beta secretion and cleaved IL-1beta 
      expression in the primary astrocytes. DISCUSSION AND CONCLUSION: These results 
      indicated the anti-inflammatory effects of 5-methylthiopentyl isothiocyanate 
      (berteroin) by regulating NLRP3 inflammasome activation, suggesting that 
      berteroin could be the potential natural drug candidate for the regulation of 
      inflammation.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Choi, Su-Bin
AU  - Choi SB
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
FAU - Kim, Ji-Hye
AU  - Kim JH
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
FAU - Kwon, Sehee
AU  - Kwon S
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
FAU - Ahn, Na-Hyun
AU  - Ahn NH
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
FAU - Lee, Joo-Hee
AU  - Lee JH
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
FAU - Yang, Woong-Suk
AU  - Yang WS
AD  - National Institute for Nanomaterials Technology (NINT), POSTECH, 77, Pohang 
      37673, Republic of Korea.
FAU - Kim, Cheorl-Ho
AU  - Kim CH
AD  - Department of Biological Sciences, Sungkyunkwan University and Samsung Advanced 
      Institute of Health Science and Technology, Suwon 1649, Republic of Korea.
FAU - Yang, Seung-Hoon
AU  - Yang SH
AUID- ORCID: 0000-0002-6880-8861
AD  - Department of Biomedical Engineering, College of Life Science and Biotechnology, 
      Dongguk University, Seoul 04620, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20230824
PL  - Netherlands
TA  - Curr Pharm Biotechnol
JT  - Current pharmaceutical biotechnology
JID - 100960530
SB  - IM
OTO - NOTNLM
OT  - 5-Methylthiopentyl isothiocyanate
OT  - ASC spec
OT  - NLRP3 inflammasome
OT  - astrocyte
OT  - primary bone marrow-derived macropahges (BMDMs)
OT  - pro-inflammatory cytokine
OT  - sulforaphane analogue (berteroin)
EDAT- 2023/08/25 12:43
MHDA- 2023/08/25 12:43
CRDT- 2023/08/25 08:13
PHST- 2023/01/06 00:00 [received]
PHST- 2023/04/25 00:00 [revised]
PHST- 2023/05/26 00:00 [accepted]
PHST- 2023/08/25 12:43 [medline]
PHST- 2023/08/25 12:43 [pubmed]
PHST- 2023/08/25 08:13 [entrez]
AID - CPB-EPUB-134007 [pii]
AID - 10.2174/1389201024666230824093927 [doi]
PST - aheadofprint
SO  - Curr Pharm Biotechnol. 2023 Aug 24. doi: 10.2174/1389201024666230824093927.