PMID- 37623667
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230829
IS  - 2079-4983 (Print)
IS  - 2079-4983 (Electronic)
IS  - 2079-4983 (Linking)
VI  - 14
IP  - 8
DP  - 2023 Aug 11
TI  - Composite Hydrogels of Ultrasound-Assisted-Digested Formic Acid-Decellularized 
      Extracellular Matrix and Sacchachitin Nanofibers Incorporated with Platelet-Rich 
      Plasma for Diabetic Wound Treatment.
LID - 10.3390/jfb14080423 [doi]
LID - 423
AB  - In this study, an ultrasound-assisted digestion method of a formic 
      acid-decellularized extracellular matrix (dECM) of porcine skin was developed and 
      optimized to form UdECM hydrogels for diabetic wound healing. Results 
      demonstrated that ultrasonication improved the extraction rate of collagen from 
      dECM samples, preserved the collagen content of dECM, reduced residual cells, and 
      extracted greater DNA contents. Scanning electron microscope (SEM) analyses were 
      performed, which demonstrated the optimal porosity on the surface and density of 
      the cross-section in the hydrogel structure, which could control the release of 
      growth factors embedded in UdECM hydrogels at desirable rates to boost wound 
      healing. A wound-healing study was conducted with six different composite 
      hydrogels, both empty materials and materials enriched with rat platelet-rich 
      plasma (R-PRP), sacchachitin nanofibers (SCNFs), and TEMPO-oxidized sacchachitin 
      in diabetic rats. The assessment based on scars stained with hematoxylin and 
      eosin (H&E), Masson's trichrome (MT), and a cluster of differentiation 31 (CD31) 
      staining showed that the UdECM/SC/R-PRP treatment group had the most significant 
      efficacy of promoting healing and even recovery of diabetic wounds to normal 
      tissues. UdECM/R-PRP and UdECM/SCNFs demonstrated better healing rates than UdECM 
      hydrogel scaffolds, which had only recovered 50% resemblance to normal skin. 
      Treatment with both UdECM/TEMPO 050 and UdECM/TEMPO 050/R-PRP hydrogel scaffolds 
      was ranked last, with even poorer efficacy than UdECM hydrogels. In summary, 
      formulated UdECM and SCNF hydrogels loaded with PRP showed synergistic effects of 
      accelerating wound healing and ultimately stimulating the wound to recover as 
      functional tissues. This newly UdECM/SCNF composite hydrogel has promising 
      potential for healing and regenerating diabetic wounds.
FAU - Lin, Chien-Ju
AU  - Lin CJ
AD  - School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 
      80708, Taiwan.
FAU - Lin, Hong-Liang
AU  - Lin HL
AD  - School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 
      80708, Taiwan.
FAU - You, Wen-Chen
AU  - You WC
AD  - School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Ho, Hsiu-O
AU  - Ho HO
AD  - School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Sheu, Ming-Thau
AU  - Sheu MT
AUID- ORCID: 0000-0003-1995-8369
AD  - School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Chen, Ling-Chun
AU  - Chen LC
AD  - Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of 
      Medical Technology, Hsinchu 30015, Taiwan.
FAU - Cheng, Wei-Jie
AU  - Cheng WJ
AUID- ORCID: 0009-0006-2118-1252
AD  - School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, 
      Taiwan.
LA  - eng
GR  - MOST 111-2221-E-264 -002 -MY2/National Science and Technology Council/
PT  - Journal Article
DEP - 20230811
PL  - Switzerland
TA  - J Funct Biomater
JT  - Journal of functional biomaterials
JID - 101570734
PMC - PMC10455550
OTO - NOTNLM
OT  - decellularized extracellular matrix
OT  - hydrogel
OT  - platelet-rich plasma
OT  - sacchachitin
OT  - ultrasound
OT  - wound dressing
COIS- The funders had no role in the design of the study; in the collection, analyses, 
      or interpretation of data; in the writing of the manuscript; or in the decision 
      to publish the results.
EDAT- 2023/08/25 12:42
MHDA- 2023/08/25 12:43
PMCR- 2023/08/11
CRDT- 2023/08/25 09:14
PHST- 2023/06/26 00:00 [received]
PHST- 2023/08/08 00:00 [revised]
PHST- 2023/08/10 00:00 [accepted]
PHST- 2023/08/25 12:43 [medline]
PHST- 2023/08/25 12:42 [pubmed]
PHST- 2023/08/25 09:14 [entrez]
PHST- 2023/08/11 00:00 [pmc-release]
AID - jfb14080423 [pii]
AID - jfb-14-00423 [pii]
AID - 10.3390/jfb14080423 [doi]
PST - epublish
SO  - J Funct Biomater. 2023 Aug 11;14(8):423. doi: 10.3390/jfb14080423.