PMID- 37623736
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230829
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 21
IP  - 8
DP  - 2023 Aug 18
TI  - Echinochrome Ameliorates Physiological, Immunological, and Histopathological 
      Alterations Induced by Ovalbumin in Asthmatic Mice by Modulating the Keap1/Nrf2 
      Signaling Pathway.
LID - 10.3390/md21080455 [doi]
LID - 455
AB  - Asthma is a persistent inflammatory disease of the bronchi characterized by 
      oxidative stress, airway remodeling, and inflammation. Echinochrome (Ech) is a 
      dark-red pigment with antioxidant and anti-inflammatory activities. In this 
      research, we aimed to investigate the effects of Ech against asthma-induced 
      inflammation, oxidative stress, and histopathological alterations in the spleen, 
      liver, and kidney in mice. Mice were divided into four groups (n = 8 for each): 
      control, asthmatic, and asthmatic mice treated intraperitoneally with 0.1 and 1 
      mg/kg of Ech. In vitro, findings confirmed the antioxidant and anti-inflammatory 
      activities of Ech. Ech showed antiasthmatic effects by lowering the serum levels 
      of immunoglobulin E (IgE), interleukin 4 (IL-4), and interleukin 1beta (IL-1beta). It 
      attenuated oxidative stress by lowering malondialdehyde (MDA) and nitric oxide 
      (NO) contents and increasing reduced glutathione (GSH), superoxide dismutase 
      (SOD), glutathione-s-transferase (GST), and catalase (CAT) in the liver, spleen, 
      and kidney. Moreover, it protected asthma-induced kidney and liver functions by 
      increasing total protein and albumin and decreasing aspartate aminotransferase 
      (AST), alanine aminotransferase (ALT), creatinine, urea, and uric acid levels. 
      Additionally, it ameliorated histopathological abnormalities in the lung, liver, 
      spleen, and kidney. Additionally, molecular docking studies were used to examine 
      the interactions between Ech and Kelch-like ECH-associated protein 1 (Keap1). PCR 
      and Western blot analyses confirmed the association of Ech with Keap1 and, 
      consequently, the regulatory role of Ech in the Keap1-(nuclear factor erythroid 
      2-related factor 2) Nrf2 signaling pathway in the liver, spleen, and kidney. 
      According to our findings, Ech prevented asthma and its complications in the 
      spleen, liver, and kidney. Inhibition of inflammation and oxidative stress are 
      two of echinochrome's therapeutic actions in managing asthma by modulating the 
      Keap1/Nrf2 signaling pathway.
FAU - Abdelmawgood, Islam Ahmed
AU  - Abdelmawgood IA
AD  - Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
FAU - Mahana, Noha Ahmed
AU  - Mahana NA
AUID- ORCID: 0000-0001-7905-7787
AD  - Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
FAU - Badr, Abeer Mahmoud
AU  - Badr AM
AD  - Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
FAU - Mohamed, Ayman Saber
AU  - Mohamed AS
AD  - Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
FAU - Al Shawoush, Abdeljalil Mohamed
AU  - Al Shawoush AM
AD  - Zoology Department, Faculty of Science, Sirte University, Sirte 128123, Libya.
FAU - Atia, Tarek
AU  - Atia T
AD  - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, 
      Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
FAU - Abdelrazak, Amir Elhadi
AU  - Abdelrazak AE
AD  - Department of Medical Physiology, Medicine Program, Batterjee Medical College, 
      Jeddah 21442, Saudi Arabia.
FAU - Sakr, Hader I
AU  - Sakr HI
AUID- ORCID: 0000-0003-2917-2423
AD  - Department of Medical Physiology, Medicine Program, Batterjee Medical College, 
      Jeddah 21442, Saudi Arabia.
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo 
      11562, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20230818
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 9006-59-1 (Ovalbumin)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Antioxidants)
RN  - 0 (Keap1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Ovalbumin
MH  - Kelch-Like ECH-Associated Protein 1
MH  - *NF-E2-Related Factor 2
MH  - Antioxidants/pharmacology
MH  - Molecular Docking Simulation
MH  - *Asthma/drug therapy
MH  - Signal Transduction
MH  - Inflammation
PMC - PMC10455754
OTO - NOTNLM
OT  - Keap1
OT  - Nrf2
OT  - asthma
OT  - echinochrome
OT  - inflammation
OT  - ovalbumin
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/25 12:42
MHDA- 2023/08/28 06:42
PMCR- 2023/08/18
CRDT- 2023/08/25 09:15
PHST- 2023/07/22 00:00 [received]
PHST- 2023/08/15 00:00 [revised]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/08/28 06:42 [medline]
PHST- 2023/08/25 12:42 [pubmed]
PHST- 2023/08/25 09:15 [entrez]
PHST- 2023/08/18 00:00 [pmc-release]
AID - md21080455 [pii]
AID - marinedrugs-21-00455 [pii]
AID - 10.3390/md21080455 [doi]
PST - epublish
SO  - Mar Drugs. 2023 Aug 18;21(8):455. doi: 10.3390/md21080455.