PMID- 37626799
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230828
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 8
DP  - 2023 Aug 18
TI  - SLC22A3 rs2048327 Polymorphism Is Associated with Diabetic Retinopathy in 
      Caucasians with Type 2 Diabetes Mellitus.
LID - 10.3390/biomedicines11082303 [doi]
LID - 2303
AB  - The Solute Carrier Family 22 Member 3 (SLC22A3) is a high-capacity, low-affinity 
      transporter for the neurotransmitters norepinephrine, epinephrine, dopamine, 
      serotonin, and histamine. SLC22A3 plays important roles in interorgan and 
      interorganism small-molecule communication, and also regulates local and overall 
      homeostasis in the body. Our aim was to investigate the association between the 
      rs2048327 gene polymorphism and diabetic retinopathy (DR) in Slovenian patients 
      with type 2 diabetes mellitus (T2DM). We also investigated SLC22A3 expression in 
      the fibrovascular membranes (FVMs) of patients with proliferative DR (PDR). Our 
      study involved 1555 unrelated Caucasians with T2DM with a defined ophthalmologic 
      status: 577 of them with DR as the study group, and 978 without DR as the control 
      group. The investigated polymorphisms were genotyped using the KASPar genotyping 
      assay. The expression of SLC22A3 (organic cation transporter 3-OCT3) was examined 
      via immunohistochemistry in human FVM from 16 patients with PDR. The C allele and 
      CC genotype frequencies of the rs2048327 polymorphism were significantly higher 
      in the study group compared to the controls. The logistic regression analysis 
      showed that the carriers of the CC genotype in the recessive genetic models of 
      this polymorphism have a 1.531-fold increase (95% CI 1.083-2.161) in the risk of 
      developing DR. Patients with the C allele of rs2048327 compared to the 
      homozygotes for the wild type T allele exhibited a higher density of SLC22A3 
      (OCT3)-positive cells (10.5 +/- 4.5/mm(2) vs. 6.1 +/- 2.7/mm(2), respectively; p < 
      0.001). We showed the association of the rs2048327 SLC22A3 gene polymorphism with 
      DR in a Slovenian cohort with type 2 diabetes mellitus, indicating its possible 
      role as a genetic risk factor for the development of this diabetic complication.
FAU - Grbic, Emin
AU  - Grbic E
AD  - Department of Physiology, Faculty of Medicine, University of Tuzla, 75000 Tuzla, 
      Bosnia and Herzegovina.
FAU - Globocnik Petrovic, Mojca
AU  - Globocnik Petrovic M
AD  - Clinic of Ophthalmology, Ljubljana University Medical Centre, 1000 Ljubljana, 
      Slovenia.
FAU - Cilensek, Ines
AU  - Cilensek I
AD  - Institute of Histology and Embryology, Faculty of Medicine, University of 
      Ljubljana, 1000 Ljubljana, Slovenia.
FAU - Petrovic, Danijel
AU  - Petrovic D
AUID- ORCID: 0000-0001-6637-2542
AD  - Institute of Histology and Embryology, Faculty of Medicine, University of 
      Ljubljana, 1000 Ljubljana, Slovenia.
LA  - eng
PT  - Journal Article
DEP - 20230818
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10452275
OTO - NOTNLM
OT  - SLC22A3
OT  - diabetic retinopathy
OT  - immunohistochemistry
OT  - rs2048327
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:46
MHDA- 2023/08/26 10:47
PMCR- 2023/08/18
CRDT- 2023/08/26 01:02
PHST- 2023/07/19 00:00 [received]
PHST- 2023/08/08 00:00 [revised]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/08/26 10:47 [medline]
PHST- 2023/08/26 10:46 [pubmed]
PHST- 2023/08/26 01:02 [entrez]
PHST- 2023/08/18 00:00 [pmc-release]
AID - biomedicines11082303 [pii]
AID - biomedicines-11-02303 [pii]
AID - 10.3390/biomedicines11082303 [doi]
PST - epublish
SO  - Biomedicines. 2023 Aug 18;11(8):2303. doi: 10.3390/biomedicines11082303.