PMID- 37626897
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230829
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 16
DP  - 2023 Aug 17
TI  - Alzheimer's Disease-like Pathological Features in the Dorsal Hippocampus of 
      Wild-Type Rats Subjected to Methionine-Diet-Evoked Mild Hyperhomocysteinaemia.
LID - 10.3390/cells12162087 [doi]
LID - 2087
AB  - Multifactorial interactions, including nutritional state, likely participate in 
      neurodegeneration's pathogenesis and evolution. Dysregulation in methionine (Met) 
      metabolism could lead to the development of hyperhomocysteinaemia (hHcy), playing 
      an important role in neuronal dysfunction, which could potentially lead to the 
      development of Alzheimer's disease (AD)-like pathological features. This study 
      combines proton magnetic resonance spectroscopy ((1)H MRS) with 
      immunohistochemical analysis to examine changes in the metabolic ratio and 
      histomorphological alterations in the dorsal rat hippocampus (dentate gyrus-DG) 
      subjected to a high Met diet. Male Wistar rats (420-480 g) underwent hHcy evoked 
      by a Met-enriched diet (2 g/kg of weight/day) lasting four weeks. Changes in the 
      metabolic ratio profile and significant histomorphological alterations have been 
      found in the DG of hHcy rats. We have detected increased morphologically changed 
      neurons and glial cells with increased neurogenic markers and apolipoprotein E 
      positivity parallel with a diminished immunosignal for the N-Methyl-D-Aspartate 
      receptor 1 in hHcy animals. A Met diet induced hHcy, likely via direct Hcy 
      neurotoxicity, an interference with one carbon unit metabolism, and/or epigenetic 
      regulation. These conditions lead to the progression of neurodegeneration and the 
      promotion of AD-like pathological features in the less vulnerable hippocampal DG, 
      which presents a plausible therapeutic target.
FAU - Kovalska, Maria
AU  - Kovalska M
AUID- ORCID: 0000-0001-6802-5309
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Hnilicova, Petra
AU  - Hnilicova P
AUID- ORCID: 0000-0003-1529-1675
AD  - Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Department of Anatomy, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
FAU - Adamkov, Marian
AU  - Adamkov M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Kovalska, Libusa
AU  - Kovalska L
AD  - Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, 
      Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AD  - Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230817
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - AE28F7PNPL (Methionine)
RN  - 73JWT2K6T3 (Racemethionine)
SB  - IM
MH  - Male
MH  - Rats
MH  - Animals
MH  - Methionine
MH  - *Alzheimer Disease
MH  - Epigenesis, Genetic
MH  - Rats, Wistar
MH  - Racemethionine
MH  - Diet
MH  - Hippocampus
MH  - *Hyperhomocysteinemia
PMC - PMC10453870
OTO - NOTNLM
OT  - 1H MRS
OT  - Alzheimer's disease-like pathology
OT  - dorsal hippocampus
OT  - methionine diet
OT  - neurodegeneration
OT  - rat
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:45
MHDA- 2023/08/28 07:16
PMCR- 2023/08/17
CRDT- 2023/08/26 01:02
PHST- 2023/06/16 00:00 [received]
PHST- 2023/07/27 00:00 [revised]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/08/28 07:16 [medline]
PHST- 2023/08/26 10:45 [pubmed]
PHST- 2023/08/26 01:02 [entrez]
PHST- 2023/08/17 00:00 [pmc-release]
AID - cells12162087 [pii]
AID - cells-12-02087 [pii]
AID - 10.3390/cells12162087 [doi]
PST - epublish
SO  - Cells. 2023 Aug 17;12(16):2087. doi: 10.3390/cells12162087.