PMID- 37627050
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230828
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 15
IP  - 16
DP  - 2023 Aug 8
TI  - Genetic Profiling of Cell-Free DNA in Liquid Biopsies: A Complementary Tool for 
      the Diagnosis of B-Cell Lymphomas and the Surveillance of Measurable Residual 
      Disease.
LID - 10.3390/cancers15164022 [doi]
LID - 4022
AB  - PURPOSE: To assess the potential value of LiqBio as a complementary tool for 
      diagnosis and surveillance of BCL. METHODS: This prospective multi-center study 
      included 78 patients (25 follicular lymphomas (FL) and 53 large B-cell lymphomas 
      (LBCL)). We performed next-generation sequencing (NGS) of cfDNA LiqBio and paired 
      gDNA tissue biopsies at diagnosis and compared the mutational statuses. Also, 
      through NGS of LiqBio, we identified MRD biomarkers and compared this novel 
      LiqBio-MRD method with PET/CT in detecting MRD at follow-up. RESULTS: We 
      identified mutations in 71% of LiqBio and 95% of tissue biopsies, and found a 
      correlation between variant allele frequency of somatic mutations. Additionally, 
      we identified mutations in 73% of LiqBio from patients with no available tissue 
      samples or no mutations in them. Regarding the utility of LiqBio-MRD as a dynamic 
      monitoring tool, when compared with the PET/CT method, a lower sensitivity was 
      observed for LiqBio-MRD at 92.3% (vs. 100% for PET/CT), but a higher specificity 
      of 91.3% (vs. 86.9% for PET/CT). CONCLUSION: Genetic profiling of tumor cfDNA in 
      plasma LiqBio is a complementary tool for BCL diagnosis and MRD surveillance.
FAU - Figaredo, Gloria
AU  - Figaredo G
AUID- ORCID: 0000-0002-4031-9601
AD  - Department of Haematology, Hospital Universitario de Toledo, Av. del Rio 
      Guadiana, 45007 Toledo, Spain.
FAU - Martin-Munoz, Alejandro
AU  - Martin-Munoz A
AUID- ORCID: 0000-0002-5180-5760
AD  - Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain.
FAU - Barrio, Santiago
AU  - Barrio S
AD  - Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain.
AD  - Computational Science Department, Carlos III University, Ronda de Toledo, 1, 
      28005 Madrid, Spain.
FAU - Parrilla, Laura
AU  - Parrilla L
AUID- ORCID: 0000-0003-4740-8057
AD  - Department of Haematology, Hospital Universitario de Toledo, Av. del Rio 
      Guadiana, 45007 Toledo, Spain.
FAU - Campos-Martin, Yolanda
AU  - Campos-Martin Y
AD  - Biobank Department, Hospital Universitario de Toledo, Av. del Rio Guadiana, 45007 
      Toledo, Spain.
FAU - Poza, Maria
AU  - Poza M
AD  - Haematology Department, Hospital Universitario 12 de Octubre, Avda. de Cordoba, 
      s/n, 28041 Madrid, Spain.
FAU - Rufian, Laura
AU  - Rufian L
AD  - Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain.
AD  - Haematology Department, Hospital Universitario 12 de Octubre, Avda. de Cordoba, 
      s/n, 28041 Madrid, Spain.
FAU - Algara, Patrocinio
AU  - Algara P
AD  - Genetics Department, Hospital Universitario de Toledo, Av. del Rio Guadiana, 
      45007 Toledo, Spain.
FAU - De La Torre, Marina
AU  - De La Torre M
AD  - Department of Haematology, Hospital Universitario de Toledo, Av. del Rio 
      Guadiana, 45007 Toledo, Spain.
FAU - Jimenez Ubieto, Ana
AU  - Jimenez Ubieto A
AD  - Haematology Department, Hospital Universitario 12 de Octubre, Avda. de Cordoba, 
      s/n, 28041 Madrid, Spain.
FAU - Martinez-Lopez, Joaquin
AU  - Martinez-Lopez J
AUID- ORCID: 0000-0001-7908-0063
AD  - Haematology Department, Hospital Universitario 12 de Octubre, Avda. de Cordoba, 
      s/n, 28041 Madrid, Spain.
FAU - Casado, Luis-Felipe
AU  - Casado LF
AD  - Department of Haematology, Hospital Universitario de Toledo, Av. del Rio 
      Guadiana, 45007 Toledo, Spain.
FAU - Mollejo, Manuela
AU  - Mollejo M
AD  - Anatomopathology Department, Hospital Universitario de Toledo, Av. del Rio 
      Guadiana, 45007 Toledo, Spain.
LA  - eng
PT  - Journal Article
DEP - 20230808
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC10452485
OTO - NOTNLM
OT  - B-cell lymphoma
OT  - NGS
OT  - PET/CT
OT  - cfDNA
OT  - liquid biopsies
OT  - minimal residual disease
COIS- A.M.-M., S.B. and L.R. are employees of Altum Sequencing Co. S.B. and J.M.-L. are 
      equity shareholders of Altum Sequencing Co. J.M.-L. Roche advisory and L.-F.C. 
      Roche research founding. The other authors declare no conflict of interest.
EDAT- 2023/08/26 10:41
MHDA- 2023/08/26 10:42
PMCR- 2023/08/08
CRDT- 2023/08/26 01:03
PHST- 2023/07/07 00:00 [received]
PHST- 2023/07/26 00:00 [revised]
PHST- 2023/08/06 00:00 [accepted]
PHST- 2023/08/26 10:42 [medline]
PHST- 2023/08/26 10:41 [pubmed]
PHST- 2023/08/26 01:03 [entrez]
PHST- 2023/08/08 00:00 [pmc-release]
AID - cancers15164022 [pii]
AID - cancers-15-04022 [pii]
AID - 10.3390/cancers15164022 [doi]
PST - epublish
SO  - Cancers (Basel). 2023 Aug 8;15(16):4022. doi: 10.3390/cancers15164022.