PMID- 37628099
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230828
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 12
IP  - 16
DP  - 2023 Aug 18
TI  - A High-Performance Liquid Chromatography with Electrochemical Detection Method 
      Developed for the Sensitive Determination of Ascorbic Acid: Validation, 
      Application, and Comparison with Titration, Spectrophotometric, and 
      High-Performance Liquid Chromatography with Diode-Array Detection Methods.
LID - 10.3390/foods12163100 [doi]
LID - 3100
AB  - L-ascorbic acid (vitamin C, VC), an essential nutrient obtained from the diet to 
      maintain various vital signs for the human body, is a crucial indicator of food 
      quality and nutritional value. Herein, high-performance liquid chromatography 
      with electrochemical detection (HPLC-ECD) was developed and validated with the 
      advantages of higher sensitivity, simpler operation processes, and more rapid 
      detection in measuring VC levels in honey samples when compared with the common 
      methods (titration, spectrophotometric, and HPLC-DAD methods). The results of the 
      HPLC-ECD methodological validation showed that the limit of detection (LOD) was 
      0.0043 microg mL(-1); the relative standard deviations (RSDs) of the intra- and 
      inter-day values were between 2.51% and 5.15%, and the regression coefficient was 
      >0.999 in the linear range of 0.1 to 20 microg mL(-1). The validated HPLC-ECD method 
      was also successfully utilized to evaluate the VC levels in different varieties 
      of honey samples with various storage durations as well as in fruit and 
      biological samples. This study provided a perspective for the further accurate 
      determination of VC content in food and biological samples.
FAU - Wu, Fanhua
AU  - Wu F
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
FAU - Xu, Fangrui
AU  - Xu F
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
FAU - Liu, Wen
AU  - Liu W
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
FAU - Chen, Sinan
AU  - Chen S
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
FAU - Luo, Haojie
AU  - Luo H
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
FAU - Cheng, Ni
AU  - Cheng N
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
AD  - Bee Product Research Center of Shaanxi Province, Xi'an 710065, China.
FAU - Zhao, Haoan
AU  - Zhao H
AUID- ORCID: 0000-0001-7071-9385
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
AD  - Bee Product Research Center of Shaanxi Province, Xi'an 710065, China.
FAU - Cao, Wei
AU  - Cao W
AD  - College of Food Science and Technology, Northwest University, Xi'an 710069, 
      China.
AD  - Bee Product Research Center of Shaanxi Province, Xi'an 710065, China.
LA  - eng
GR  - 2022YFD1600204/National Key R&D Project of China/
GR  - No. 22NYYF047/Xi'an Science and Technology Planning Project/
GR  - TZ0389, QCYRCXM-2022-214/Shaanxi High-Level Talent Special Support Plan/
PT  - Journal Article
DEP - 20230818
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC10453043
OTO - NOTNLM
OT  - ascorbic acid
OT  - biosamples
OT  - electrochemical detection
OT  - fruit
OT  - honey
OT  - method validation
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:45
MHDA- 2023/08/26 10:46
PMCR- 2023/08/18
CRDT- 2023/08/26 01:10
PHST- 2023/07/27 00:00 [received]
PHST- 2023/08/11 00:00 [revised]
PHST- 2023/08/15 00:00 [accepted]
PHST- 2023/08/26 10:46 [medline]
PHST- 2023/08/26 10:45 [pubmed]
PHST- 2023/08/26 01:10 [entrez]
PHST- 2023/08/18 00:00 [pmc-release]
AID - foods12163100 [pii]
AID - foods-12-03100 [pii]
AID - 10.3390/foods12163100 [doi]
PST - epublish
SO  - Foods. 2023 Aug 18;12(16):3100. doi: 10.3390/foods12163100.