PMID- 37628565
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230828
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 8
DP  - 2023 Jul 25
TI  - Selection and Evaluation of Reference Genes for RT-qPCR Analysis in 
      Amorphophallus Konjac Based on Transcriptome Data.
LID - 10.3390/genes14081513 [doi]
LID - 1513
AB  - Combined with the Konjac transcriptome database of our laboratory and internal 
      reference genes commonly used in plants, the eight candidate internal reference 
      genes were screened and detected. They are the 25S ribosomal RNA gene (25S rRNA), 
      18S ribosomal RNA gene (18S rRNA), actin gene (ACT), glyceraldehyde-3-phosphate 
      dehydrogenase gene (GAPDH), ubiquitin gene (UBQ), beta-tubulin gene (beta-TUB), 
      eukaryotic elongation factor 1-alphagene(eEF-1alpha), and eukaryotic translation 
      initiation factor 4alpha-1 gene (eIF-4alpha). The results of GeNorm, Normfinder, and 
      BestKeeper were analyzed comprehensively. The data showed that the expression 
      levels of 25S rRNA, 18S rRNA, and ACT at the reproductive periods, eEF-1alpha and 
      eIF-4alpha at the nutritional periods, and eEF-1alpha, UBQ, and ACT at different leaf 
      developmental periods were stable. These identified and stable internal reference 
      genes will provide the basis for the subsequent molecular biology-related studies 
      of Konjac.
FAU - Liu, Yanli
AU  - Liu Y
AD  - College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 
      430023, China.
FAU - Zhang, Chengcheng
AU  - Zhang C
AD  - Lotus Engineering Research Center of Hubei Province, College of Life Science, 
      Wuhan University, Wuhan 430072, China.
FAU - Harijati, Nunung
AU  - Harijati N
AD  - Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya 
      University, Jl. Veteran, Malang 65145, Indonesia.
FAU - Diao, Ying
AU  - Diao Y
AUID- ORCID: 0000-0002-8598-4082
AD  - College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 
      430023, China.
FAU - Liu, Erxi
AU  - Liu E
AD  - Institute of Konjac, Enshi Academy of Agricultural Sciences, Enshi 445000, China.
FAU - Hu, Zhongli
AU  - Hu Z
AD  - College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 
      430023, China.
AD  - Lotus Engineering Research Center of Hubei Province, College of Life Science, 
      Wuhan University, Wuhan 430072, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230725
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (Actins)
SB  - IM
MH  - RNA, Ribosomal, 18S
MH  - *Amorphophallus
MH  - Transcriptome/genetics
MH  - Actins/genetics
MH  - Databases, Factual
PMC - PMC10454643
OTO - NOTNLM
OT  - Amorphophallus
OT  - RT-qPCR
OT  - gene expression
OT  - reference genes
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:43
MHDA- 2023/08/28 06:42
PMCR- 2023/07/25
CRDT- 2023/08/26 01:12
PHST- 2023/06/24 00:00 [received]
PHST- 2023/07/18 00:00 [revised]
PHST- 2023/07/21 00:00 [accepted]
PHST- 2023/08/28 06:42 [medline]
PHST- 2023/08/26 10:43 [pubmed]
PHST- 2023/08/26 01:12 [entrez]
PHST- 2023/07/25 00:00 [pmc-release]
AID - genes14081513 [pii]
AID - genes-14-01513 [pii]
AID - 10.3390/genes14081513 [doi]
PST - epublish
SO  - Genes (Basel). 2023 Jul 25;14(8):1513. doi: 10.3390/genes14081513.