PMID- 37628595 OWN - NLM STAT- MEDLINE DCOM- 20230828 LR - 20230829 IS - 2073-4425 (Electronic) IS - 2073-4425 (Linking) VI - 14 IP - 8 DP - 2023 Jul 27 TI - miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study. LID - 10.3390/genes14081543 [doi] LID - 1543 AB - Type 2 diabetes mellitus (T2DM) is a common metabolic disorder that results from complex interactions of both environmental and genetic factors. Many single nucleotide polymorphisms (SNPs), including noncoding RNA genes, have been investigated for their association with susceptibility to T2DM and its complications, with little evidence available regarding Caucasians. The aim of the present study was to establish whether four miRNA SNPs (miR-27a rs895819 T>C, miR-146a rs2910164 G>C, miR-196a2 rs11614913 C>T, and miR-499a rs3746444 A>G) are correlated with susceptibility to T2DM and/or diabetic polyneuropathy (DPN) in a Romanian population. A total of 167 adult T2DM patients and 324 age- and sex-matched healthy controls were included in our study. miRNA SNPs were detected by real-time PCR using a TaqMan genotyping assay. A significant association with T2DM was observed only for the miR-499a rs3746444 A>G SNP in all the tested models, and the frequencies of both the miR-499a rs3746444 AG and the GG genotypes were higher in the T2DM patients compared to the controls. No correlation was observed for the miR-27a rs895819 T>C, miR-146a rs2910164 G>C, or miR-196a2 rs11614913 C>T SNPs in any genetic model. When we assessed the association of these SNPs with DPN separately, we found a positive association for the miR-499a rs3746444 SNP in both codominant and dominant models (OR 6.47, 95% CI: 1.71-24.47; OR 2.30, 95% CI: 1.23-4.29, respectively). In conclusion, this study shows that miR-499a rs3746444 A>G may influence both T2DM and DPN susceptibility, with carriers of the GG genotype and the G allele being at an increased risk in the Romanian population. FAU - Burada, Emilia AU - Burada E AD - Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Department of Neurology, Clinical Hospital of Neuropsychiatry Craiova, 200473 Craiova, Romania. FAU - Rosu, Maria-Magdalena AU - Rosu MM AD - Department of Diabetes, Nutrition and Metabolic Diseases, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania. FAU - Sandu, Raluca Elena AU - Sandu RE AUID- ORCID: 0009-0007-8707-9768 AD - Department of Neurology, Clinical Hospital of Neuropsychiatry Craiova, 200473 Craiova, Romania. AD - Department of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Burada, Florin AU - Burada F AD - Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania. AD - Regional Centre of Medical Genetics Dolj, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania. FAU - Cucu, Mihai Gabriel AU - Cucu MG AD - Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania. AD - Regional Centre of Medical Genetics Dolj, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania. FAU - Streata, Ioana AU - Streata I AD - Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania. AD - Regional Centre of Medical Genetics Dolj, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania. FAU - Petre-Mandache, Bianca AU - Petre-Mandache B AD - Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania. AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Popescu-Hobeanu, Gabriela AU - Popescu-Hobeanu G AD - Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania. AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Cara, Monica-Laura AU - Cara ML AUID- ORCID: 0000-0002-5471-4717 AD - Department of Public Health, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Tuca, Anca-Maria AU - Tuca AM AD - Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Pinosanu, Elena AU - Pinosanu E AUID- ORCID: 0009-0001-6611-1083 AD - Department of Neurology, Clinical Hospital of Neuropsychiatry Craiova, 200473 Craiova, Romania. AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Albu, Carmen Valeria AU - Albu CV AUID- ORCID: 0009-0008-9603-5860 AD - Department of Neurology, Clinical Hospital of Neuropsychiatry Craiova, 200473 Craiova, Romania. AD - Department of Neurology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230727 PL - Switzerland TA - Genes (Basel) JT - Genes JID - 101551097 RN - 0 (MicroRNAs) SB - IM MH - Adult MH - Humans MH - *Diabetes Mellitus, Type 2/complications/genetics MH - *Diabetic Neuropathies/genetics MH - Romania MH - Polymorphism, Single Nucleotide MH - *MicroRNAs/genetics PMC - PMC10454730 OTO - NOTNLM OT - diabetic polyneuropathy OT - genotype OT - microRNAs OT - single nucleotide polymorphism OT - type 2 diabetes mellitus COIS- The authors declare no conflict of interest. EDAT- 2023/08/26 10:47 MHDA- 2023/08/28 07:16 PMCR- 2023/07/27 CRDT- 2023/08/26 01:13 PHST- 2023/07/06 00:00 [received] PHST- 2023/07/20 00:00 [revised] PHST- 2023/07/25 00:00 [accepted] PHST- 2023/08/28 07:16 [medline] PHST- 2023/08/26 10:47 [pubmed] PHST- 2023/08/26 01:13 [entrez] PHST- 2023/07/27 00:00 [pmc-release] AID - genes14081543 [pii] AID - genes-14-01543 [pii] AID - 10.3390/genes14081543 [doi] PST - epublish SO - Genes (Basel). 2023 Jul 27;14(8):1543. doi: 10.3390/genes14081543.